Sains Malaysiana 37(4):  429-433(2008)

 

Peningkatan Produktiviti CGtase dengan Menggunakan

Kultur Selanjar

(Increased productivity of CGTase using continuous culture)

 

 

Khosiya Sali, Aidil Abdul Hamid & Wan Mohtar Wan Yusoff 

Program Mikrobiologi, Pusat pengajian Biosains dan Bioteknologi

Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia

43600 Bangi, Selangor, Malaysia

 

 

Diserahkan:  12 April 2007 / Diterima: 14 Disember 2008

 

 

ABSTRAK

Penghasilan CGTase daripada Bacillus sp. G1 berjaya ditingkatkan dengan menggunakan sistem kultur selanjar mengatasi penghasilan daripada kultur kelompok.  Aktiviti CGTase tertinggi yang didapati dalam kultur kelompok ialah 28.1 U/ml.  Kajian kultur selanjar difokuskan kepada masa kemasukan medium segar yang berbeza (12, 24 dan 48 jam ), kadar pencairan ditetapkan pada 0.03 per jam.  Hasil menunjukkan masa memulakan pam medium segar tidak memberi perubahan yang signifikan terhadap aktiviti CGTase (25.7, 26.3 dan 26.1 U/ml masing-masing) dan produktiviti CGTase (0.77, 0.79 dan 0.78 U/ml/j masing-masing) pada keadaan mantap tetapi produktiviti CGTase (0.77 U/ml/j) akan lebih tinggi berbanding produktiviti kultur kelompok apabila masa di antara larian kultur kelompok diambil kira. Malah peningkatan berpotensi ditingkatkan lagi dengan memulakan pam medium segar lebih awal daripada 12 jam dan juga dengan meningkatkan kadar pencairan.

 

Kata kunci: Bacillus sp. G1; CGTase; kultur selanjar

 

 

ABSTRACT

 

The production of CGTase from Bacillus sp. G1 was successfully increased using continuous culture.  The highest CGTase activity in batch culture was 28.1 U/ml.  The study in continuous culture was focused on the effect of different time (12, 24 and 48 hours) to start pumping fresh medium at fixed dilution rate of 0.03 per hour.  Results showed that the pumping time did not give significant changes in CGTase activity (25.7, 26.3 and 26.1 U/ml respectively) and in CGTase productivity (0.77, 0.78 and 0.78 U/ml/h) respectively at steady state operation.  Nevertheless, the productivity (0.77 U/ml/h) of CGTase was found to be higher than that obtained in batch culture when the time interval between batch run was taken into account.  The productivity could be further increased by pumping the media earlier than 12 hours and also by increasing the dilution rate.

 

Keyword:  Bacillus sp. G1; CGTase; continuous culture

 

 

REFERENCES/RUJUKAN

 

Abdel-Naby, M.A. Rayad, R.M. & Abdel-Fattah, A.F. 2000. Biosynthesis of cyclodextrin

glucanotransferase by immobilized Bacillus amyloliquefaciens in batch and continuous culture. Biochimical Engineering Journal 5: 1-9.

Dobois, M. Gilles, L.A. Hamilton, J.K. Robers, P.A. & Smith F. 1956. Colormetric method for determanation of sugar and related substances. Analytical Chemistry 28: 350-356.

Gawande, B.N. Singh, R. K. Chunhan, A.K. Goel, A. & Patkar, A.Y. 1998. Optimization

of cyclodextrin glucanotransferase production from Bacillus firmus. Enzyme and Microbial Technology 22: 288-291.

Hanan M. I., Wan M.W.Y., Aidil A.H., Rosli M.I., Othman H. & Othman O. 2005. Optimization of medium for the Production β-Cyclodextrin glucanotransferase using Central Composite Design (CCD). Process Biochemistry 40: 753-758.

Horikoshi, K. 1979. Production and industrial application of cyclodextrin. Process Biochemistry: 26-30.

Jamuna R. & Saswathi N., Sheela R. & Ramakrishna S.V. 1993. Synthesis of Cyclodextrin glucanotransferase by Bacillus cereus for the Production of Cyclodextrin. Applied Biochem. Biotechnol 43: 163-176.

Kaneko, T. Kato, T. Nakamura, N. & Horikoshi, K. 1987. Spectrophotometric determination of cyclization activity of cyclodextrin-forming cyclomaltodextrin glucanotransferase. Journal of Japanese Society of Starch Science 34(1): 45-48.

Kunamneni, A. Prabhakar, T. Jyothi, B. & Ellaiah, P. 2007. Investigation of continuous culture cyclodextrin glucanotransferase producton by the algenate-immobilized cells of alkalofilik Bacillus sp. in an airlift reactor. Enzyme Microbial Technology 40: 1538-1542.

Pirt, J.S. 1975. Principles of microbe and cell cultivation. New York: Black Well Scientific Publication.

Rosso, A.M. Ferrarotti, S.A. Krymkiewicz, N. & Nudel, C. 2002. Optimisation of batch culture conditions for cyclodextrin glucanotransfease production from Bacillus circulans DF 9R. Microbial Cell Factories 1: 3-12.

Szejtli J. 1997. Utilization of cyclodexstrin in industrial products and processes. Journal  of Material Chemistry 7: 575-587.

Tonkova A. 1998. Bacterial cycclodextrin glucotrasferase. Enzyme and Microbial Technology 22: 678-686.

Vassileva, A. Burhan, N. Beschkov, V. Spasova, D. Radoevska, S. Ivanova, V. & Tonkova, A. 2003. Cyclodextrin glucanotransferase production by free and agar gel immobilized cell of Bacillus circulans ATCC 21783 in bioreactors. Process Biochemistry 38: 1585-1591.

Vassileva, A. Beschkov, V. Ivanova, V. & Tonkova, A. 2005. Continuous cyclodextrin glucanotransferase production by free and immobilized cell of Bacillus circulans ATCC 21783 in bioreactors. Process Biochemistry 40: 3290-3295.

Wan Mohtar W.Y., Hanan M.I., Aidil A.H., Rosli M.I. & Othman O. 2002. A potential New Isolate for β-Cyclodextrin Glucanotransferase Production. Malaysian Journal of Biochemistry and Molecular Biology 7: 13-17.