Sains
Malaysiana 38(2): 143-147(2009)
DNA Polymorphism of D1S80 Locus in Modern Malay
Sample Population of Sarawak
(DNA Polimorfisma di Lokus DIS80 dalam Sampel Populasi
Melayu Moden Sarawak)
Hairul Azman Roslan, Nur Hafizah Azizan &
Rosmawati Saat
Department of Molecular Biology, Faculty of Resource
Science and Technology,
Universiti Malaysia Sarawak, 94300 Kota Samarahan,
Sarawak, Malaysia
Received: 11 July 2007 / Accepted: 25 June 2008
ABSTRACT
The molecular genetic marker, minisatellite locus
D1S80 (1p35-p36), is a highly polymorphic variable number of tandem repeats
(VNTR). Its polymorphic nature allows for phylogenetic studies, forensic
analysis, genetic maps construction and paternity testing to be performed. A
study of the hypervariable locus D1S80 was conducted to determine the allele
frequency and distribution of this locus in modern Malay in
Sarawak
population. The polymerase chain reaction technique was employed and results were
analysed on polyacrylamide gel. A total of seventy-six DNA samples of unrelated
Malay individuals in UNIMAS were collected and examined. The VNTR analysis of
the D1S80 locus demonstrated the presence of 17 alleles in the Malay
population. Allele with the size of 577 bp (27 repeats) was determined to be
the most common in the sample population with the frequency of 0.1641, followed
by allele with the size of 561 bp (26 repeats) and 529 bp (24 repeats) whose
frequency is 0.1172 and 0.1094, respectively. The smallest allele is allele with
the size of 465 bp (20 repeats) whereas the largest is allele with the size of
753 bp (38 repeats). The sample population exhibited 57.8% heterozygosity.
Keywords: D1S80 locus; modern Malay population; polymorphism;
population genetic; Variable number of tandem repeats(VNTR)
ABSTRAK
Penanda genetik molekul, lokus minisatelit D1S80
(1p35-p36), adalah lokus “VNTR” yang tinggi kepelbagaian rupa bentuknya. Sifat
kepelbagaian bentuk ini membolehkan kajian filogenetik, analisis forensik, peta
genetik dan kajian penentuan ibu bapa dijalankan. Satu kajian dalam lokus D1S80
telah dijalankan untuk menentukan frekuensi dan distribusi lokus ini populasi
Melayu moden di Sarawak. Kajian telah menggunakan teknik amplifikasi rantai
polimeras (PCR) dan elektroporesis gel menggunakan media poliakrilamid. Sejumlah
76 sampel DNA dari individu Melayu dalam populasi UNIMAS yang tiada hubungan
saudara telah di ambil dan dikaji. Keputusan menunjukkan 17 alel diperoleh dari
populasi ini. Alel bersaiz 577 bp (27 ulangan) ialah alel yang biasa dijumpai
dengan frekuensi 0.1641 dan diikuti dengan alel bersaiz 561 bp (26 ulangan) and
529 bp (24 ulangan) dengan frekuensi masing-masing 0.1172 dan 0.1094. Alel
terkecil yang dijumpai ialah alel bersaiz 465 bp (20 ulangan) manakala alel
yang terbesar ialah alel bersaiz 753 bp (38 ulangan). Populasi ini menunjukkan
56.9% individu heterozigot.
Kata kunci: Genetik populasi; kepelbagaian rupa
bentuk; Lokus D1S80; populasi Melayu moden; VNTR
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