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Sains Malaysiana 38(3): 413-418(2009)
Pembangunan
Sensor Optik pH Berasaskan Reagen Kurkumin Terpegun
Dalam Matriks
Hibrid Sol-Gel/Kitosan Melamin
(Development of optical pH sensor based on curcumin
reagent immobilized
in sol-gel/chitosan melamine hybrid matrices)
Rosmawani Mohammad,
Musa Ahmad* & Jamaluddin Mohd Daud
Pusat Pengajian
Sains Kimia & Teknologi Makanan
Fakulti Sains dan
Teknologi, Universiti Kebangsaan Malaysia
43600 Bangi,
Selangor DE., Malaysia
ABSTRAK
Sensor optik pH berasaskan reagen kurkumin terpegun dalam matriks hibrid
sol-gel/kitosan melamin telah dibangunkan dalam kajian ini. Sensor optik pH ini
memberikan rangsangan linear pada julat
pH 9-13 (R2 = 0.9846). Seterusnya, nilai
RSD kebolehulangan adalah 7.38% - 9.65%, manakala nilai RSD kebolehasilan pula adalah
2.18% - 4.72%. Kestabilanfoto yang baik bagi reagen kurkumin terpegun
diperolehi apabila filem disimpan dalam persekitaran gelap dengan nilai RSD
3.69% untuk tempoh kajian 3 minggu. Tiada kesan histerisis diperhatikan apabila
sensor digunakan untuk mengukur pH dalam kitaran pH 2-12-2. Masa rangsangan
sensor adalah 10-20 saat. Penentuan nilai pH bagi beberapa sampel sebenar
menggunakan sensor pH yang dihasilkan dalam kajian ini menunjukkan tiada
perbezaan secara signifikan pada nilai pH yang diperolehi apabila dibandingkan
dengan keputusan yang diperolehi menggunakan meter pH.
Kata kunci: Hibrid sol-gel/kitosan melamin; kurkumin; sensor optik pH
ABSTRAK
An optical pH sensor has been
developed using curcumin reagent immobilized in sol-gel/chitosan-melamine
hybrid matrices. The optical pH sensor gave a linear response at range of pH 9-13 (R2 = 0.9846). Relative standard deviation (RSD) values of reproducibility were 7.38% - 9.65%, while RSD
values of repeatability were 2.18% - 4.72%. Good photostability was obtained
for immobilized curcumin reagent when the films were kept in dark surrounding
with RSD value 3.69% for 3 weeks. No hysteresis effect was
observed when the sensor was used to measure pH in cycle from pH 2-12-2. The
response time of the sensor was within 10-20 s. Determination of pH value in
real samples by using pH sensor developed in this study showed no significance
differences when compared with results obtained by using conventional pH meter.
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