Sains Malaysiana 38(5)(2009): 761–766

Infeksi Plasmodium berghei dan Kesannya ke atas Pengisyaratan MAP Kinase Eritrosit Perumah

(Plasmodium berghei Infection and its Effect on MAP Kinase Signaling in its Erythrocyte Host)

Mohd Fakharul Zaman Raja Yahya

Pusat Pengajian Bioperubatan & Kesihatan

Kolej Universiti Kejururawatan & Kesihatan Masterskill

43200 Cheras, Selangor Darul Ehsan, Malaysia

Hasidah Mohd Sidek*

Pusat Pengajian Biosains & Bioteknologi

Fakulti Sains & Teknologi, Universiti Kebangsaan Malaysia

43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

Received: 24 June 2008 / Accepted: 12 March 2009

ABSTRAK

Kajian ini melibatkan pemantauan perkembangan parasitemia dan taburan morfologi Plasmodium berghei sewaktu infeksi parasit dalam mencit, serta penentuan kesan infeksi P. berghei ke atas pengisyaratan MAP kinase eritrosit perumah. Analisis mikroskop ke atas slaid calitan darah terwarna-Giemsa yang disediakan daripada mencit terinfeksi-P. berghei (strain PZZ1/00) menunjukkan darjah parasitemia mencapai sehingga 70% dalam masa dua minggu selepas penyuntikan parasit. Morfologi cecincin dan trofozoit parasit dicerap dengan jelas sepanjang tempoh infeksi manakala morfologi skizon parasit hanya dicerap dengan ketara selepas hari ketiga selepas penyuntikan parasit. Pemblotan Western [antibodi primer: anti-MAP kinase (ERK-1/2 tak terfosfat) monoklon; antibodi sekunder: anti-IgG, poliklon terkonjugat-HRP] ke atas protein sitosol eritrosit terinfeksi-P. berghei (70% parasitemia) susulan pemisahan SDS-PAGE menunjukkan bahawa keamatan protein imunoreaktif-MAP kinase eritrosit berberat molekul 42 dan 44 kDa didapati meningkat secara signifikan (p<0.05) pada 70% iaitu peningkatan sebanyak 21.5% dan 22.3% masing-masing berbanding sampel kawalan tanpa infeksi. Samada kesan infeksi P. berghei (70% parasitemia) ke atas pengisyaratan MAP kinase perumah ini berkaitan dengan pengaktifan enzim ini perlu dikaji dengan lebih lanjut.

Kata kunci: MAP kinase; malaria; Plasmodium berghei; transduksi isyarat

ABSTRACT

The present investigation involves monitoring the development of parasitemia and the distribution of Plasmodium berghei morphologies during parasite infection in mice as well as the effect P. berghei infection on MAP kinase signaling in its erythrocyte host. Microscopic analyses of Giemsa-stained blood films prepared from P. berghei (strain PZZ1/00)-infected mice showed that the level of parasitemia reached up to 70% two weeks after inoculation of the parasite. Parasite ring and trophozoite forms were clearly detected in the blood films throughout the study period while the schizont form was visibly observed on day 3 post-inoculation. Western blotting [primary antibody: monoclonal anti-MAP kinase (non-phosphorylated ERK-1/2); secondary antibody: HRP-conjugated polyclonal anti-IgG] carried out on SDS-PAGE-separated cytosolic protein samples from P. berghei-infected (70% parasitemia) erythrocytes showed that the levels of both 42 and 44 kDa MAP kinase-immunoreactive proteins increased significantly (p<0.05) at 70% parasitemia, by up to 21.5% and 22.3% respectively as compared to non-infected control samples. Whether the effect of P. berghei infection at 70% parasitemia on host MAP kinase signaling is related to the MAP kinase activation remains to be further investigated.

Keyword: Malaria; MAP kinase; Plasmodium berghei; signal transduction

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*Corresponding author; email: hasidah@ukm.my