Sains Malaysiana 39(1)(2010): 129–134

 

Pengklonan dan Pencirian Klon UKMCSyang Mengekodkan

Kapsaisin sintase (Cs)

(Cloning and Characterisation of CUKMCS Clone Encoding Capsaicin Synthase)

 

Nurulhikma Md. Isa

Institut Biologi Sistem, Universiti Kebangsaan Malaysia

43600, UKM Bangi, Selangor D.E., Malaysia

 

Ismanizan Ismail & Zamri Zainal*

Pusat Pengajian Biosains dan Bioteknologi, Fakulti Sains dan Teknologi

Universiti Kebangsaan Malaysia 43600, UKM Bangi, Selangor D.E.

Malaysia

 

Received: 3 February 2009 / Accepted: 21 April 2009

 

ABSTRAK

 

Kapsaisinoid merupakan alkaloid yang memberikan ciri kepedasan pada cili serta khusus pada genus Capsicum. Sebatian kapsaisinoid terdiri daripada dua komponen utama iaitu kapsaisin dan dihidrokapsaisin. Dalam kajian ini, pengklonan cDNA Kapsaisin sintase (Cs) telah berjaya dilakukan menerusi kaedah transkripsi berbalik PCR (RT-PCR) dan klon cDNA tersebut dinamakan CUKMCS yang bersaiz 981 pb. Pencarian homologi menggunakan program blastx dan blastp yang terdapat pada pangkalan data NCBI mendapati CUKMCS mempunyai persamaan yang sangat tinggi terhadap Cs pada Capsicum frutescens, Capsicum annuum dan Capsicum chacoense. Saiz ramalan protein CUKMCS diangggarkan sekitar 36 kDa. Penentuan pengekspresan transkrip Cs pada 5 tisu yang berbeza mendapati transkrip dikesan pada tisu plasenta, mesokarp dan biji manakala tiada transkrip Cs dikesan pada daun dan akar.

 

Kata kunci: Kapsaisin sintase Cs; capsium frutescens; kapsaisinoid; transkrip mRNA; Capsicum RT-PCR

 

ABSTRACT

 

Capsaicinoid, the main alkaloid compound that gives pungent taste to peppers is restricted to the genus of Capsicum. It consists of two major components which are capsaicin and dihydrocapsaicin. Here we reported the isolation of capsaicin synthase (Cs) cDNA via RT-PCR method and the resulting cloned was named as CUKMCS with the expected size of 981 bp. Homology search via the blastx and blastp program from the NCBI database showed that CUKMCS has a high homology to the Cs in Capsicum frutescens, Capsicum annuum and Capsicum chacoense. The predicted size of CUKMCS protein is around 36 kDa. Transcripts expression in 5 different tissues revealed that Cs transcripts were detected in the placental tissue, mesocarp and seed while no corresponding expression was detected in the leaf and root.

 

Keywords: Capsaicin synthase Cs; capsaicinoid;  mRNA transcript; Capsicum frutescens

 

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*Pengarang untuk surat-menyurat; email: zz@ukm.my