Sains Malaysiana 39(1)(2010): 141–144

 

Kesan Pengudaraan dan Pencairan ke Atas Penghasilan Bioetanol

Secara Selanjar dalam Bioreaktor Padat oleh Saccharomyces cerevisiae

(Effect of Aeration and Dilution on Continuous Bioethanol Production  in a Packed-bed Bioreactor by Saccharomyces cerevisiae)

 

Ng Kee Wee, Aidil Abdul Hamid, Mohd Sahaid Kalil & Wan Mohtar Wan Yusoff*

Pusat Pengajian Biosains dan Bioteknologi

Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia

43600 UKM Bangi, Selangor D.E., Malaysia

 

Received: 12 June 2008 / Accepted: 6 July 2009

 

 

ABSTRAK

 

Pertumbuhan sel secara selanjar pada keadaan mantap telah diperolehi dalam pengkulturan dengan bioreaktor padat. Produktiviti (0.02 g/L/j) bioetanol yang paling tinggi diperolehi adalah pada pengudaraan 0.003 vvm. Produktiviti bioetanol didapati meningkat dengan peningkatan kadar pencairan. Produktiviti tertinggi sebanyak 0.037 g/L/j direkod semasa kadar pencairan (D) 0.05 per jam. Penghasilan bioetanol secara selanjar telah berjaya diselenggarakan pada keadaan tidak 100% anaerobik. Pengudaraan yang terbaik untuk produktiviti bioetanol dalam keadaan seimbang pertumbuhan sel dan penghasilan bioetanol ialah pada 0.003 vvm.

 

Kata kunci: bioetanol; Saccharomyces cerevisiae; sel terperangkap

 

ABSTRACT

 

Stability of cell growth was achieved continuously at a steady state in a packed-bed bioreactor. The highest productivity of ethanol was achieved (0.02 g/L/h) when 0.003 vvm was employed. The productivity of bioethanol increases when dilution rate increases. The highest production of 0.037 g/L/h was recorded when the dilution rate (D) was at 0.05 per hour. The production of bioethanol was sucessfully maintained in a non 100% anaerobic condition. The best aeration for the continous production of bioethanol in a condition of steady state growth was at an aeration rate of 0.003 vvm.

 

Keywords: Bioethanol; entraped cell; Saccharomyces cerevisiae

 

REFERENCES

 

Andreasen, A.A. & Stier, T.J.B. 1953. Anaerobic nutrition of Saccharomyces cerevisiae. I. Ergosterol requirement for growth in a defined medium. Journal of Cellular and Comparative Physiology 41: 23-36.

Andreasen, A.A. & Stier, T.J.B. 1954. Anaerobic nutrition of Saccharomyces cerevisiae. II. Unsaturated fatty acid requirement for growth in a defined medium. Journal of Cellular and Comparative Physiology 43: 271-281.

Atkinson, B., Black, G., Lewis, P. & Pinches, A. 1979. Biological particles of given size, shape and density for use in biological reactors. Biotechnology and Bioengineering 21: 193-200.

Baptista, C.M.S.G., Coias, J.M.A., Oliveira, A.C.M., Oliveira, N.M.C., Rocha, J.M.S., Dempsey, M.J., Lannigan, K.C. & Benson, P.S. 2006. Natural immobilization of microorganisms for continuous ethanol production. Enzyme and Microbial Technology 78: 321-330.

Dias, S.M.M., Novais, J.M. & Cabral, J.M.S. 1982. Immobilization of yeast on titanium activated inorganic supports. Biotechnology Letter 4: 203-208.

Godin F., Casas C., Castello B. & Sola C. 1987. Immobilized cells: behaviour of carrageenen entrapped yeast during continuous ethanol fermentation. Applied Microbiology Biotechnology 26: 342-346.

Najafpour, G., Younesi, H. & Ku Ismail, K.S. 2004. Etahnol fermentation in an immobilized cell reactor using Saccharomyces cerevisiae. Bioresource Technology 92: 251-260.

Nedovic, V.A., Pesic, R., Leskosek-Cukalovic, L., Laketic, D. & Vunjak-Novakovic, G. 1997. Analysis of liquid axial dispersion in an anternal loop bioreactor for beer fermentation with immobilized yeast cells. Proceedings of European Conference on Fluids 2: 627-634.

Peterson, G.L. 1977. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Analytical Biochemistry 83: 346-356.

Pilkingston, P.H., Margaritis, A., Mensour, N.A. & Russell, I. 1998. Fundamentals of immobilized yeast cells for continuous beer fermentation. A review. Journal of Institute Brewing 104: 19-31.

Sablayrolles, J.M., Salmon, J.M. & Barre, P. 1996. Carences nutritionnelles des mou^ ts. Efficacite« des ajouts combine« s d'oxyge`ne et d'azote ammoniacal. Revue Franc- aise d'Oenologie 159: 25-32.

Shen, H.Y., Moonjai, N., Verstrepen, K.J. & Delvaux, F.R. 2003. Impact of attachment immobilization on yeast physiology and fermentation performance. Journal of American Society Brewing Chemists 61(2): 79-87.

Virkajarvi, I. & Pohjala, N. 2000. Primary fermentation with immobilized yeast: Some effect of carrier materials on the flavor of the beer. Journal of Institute Brewing 106: 311-318.

 

*Corresponding author; email: wantar@ukm.my