Sains Malaysiana 39(2)(2010): 315–320

Hydrogen Peroxide Stimulates Glucose Uptake in Myotube Cells via the Insulin Signaling Pathway

(Hidrogen Peroksida Merangsang Pengambilan Glukosa ke dalam Sel Miotiub Melalui Tapakjalan Pengisyaratan Insulin)

Musalmah Mazlan*, Rehanna Mansor & Wan Zurinah Wan Ngah

Department Of Biochemistry, Medical Faculty, Universiti Kebangsaan Malaysia

Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia

Received: 28 April 2009 / Accepted: 20 August 2009

ABSTRACT

Recent studies suggest that reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) may exert beneficial effects on health. Therefore this study evaluated the effects of H2O2 and gamma-tocotrienol (GTT), an antioxidant, on D-[2-3H]Glucose uptake by myotubes compared to insulin. Results showed that H2O2 and insulin significantly increased D-[2-3H]Glucose uptake. This was associated with an increased in Vmax but Michaelis constant (Km) of the transport system remained unchanged indicating that an increase in amount, rather than affinity, of the glucose transporter was involved in the process. Western blot studies confirmed that H2O2 increased the expressions of insulin sensitive glucose transporter, GLUT4 and also hexokinase (an enzyme which phosphorylates glucose and indirectly stimulates glucose uptake by cells). H2O2 and insulin also stimulated increased in PPAR-γ and IRS-1, which are proteins of the insulin transduction pathway. The present data however showed that GTT did not exert any effect on glucose uptake. Thus, the study showed that H2O2 mimicked insulin action in stimulating glucose uptake in myotube cells by influencing the insulin signaling pathway.

Keywords: Hydrogen peroxide; gamma-tocotrienol; glucose uptake; insulin action; myotube cells

ABSTRAK

Kajian-kajian terkini mencadangkan bahawa spesies oksigen reaktif (ROS) seperti hidrogen peroksida (H2O2) boleh memberi kesan berguna ke atas kesihatan. Oleh itu kajian ini menilai kesan H2O2 and gama-tokotrienol (GTT), suatu antioksidan, ke atas pengambilan D-[2-3H]Glukosa oleh sel miotiub berbanding insulin. Keputusan menunjukkan bahawa H2O2 and insulin meningkatkan pengambilan D-[2-3H]Glukosa secara signifikan. Peningkatan ini berkaitan dengan peningkatan Vmaks tetapi pemalar Michaelis (Km) sistem pengangkutan tidak berubah yang menunjukkan bahawa peningkatan bilangan dan bukannya peningkatan afiniti pengangkut glukosa yang terlibat dalam proses ini. Kajian blot Western mengesahkan yang H2O2 meningkatkan ekspresi pengangkut glukosa peka insulin, GLUT4 dan juga heksokinase (enzim yang memfosforilasi glukosa dan secara taklangsung merangsang pengambilan glukosa oleh sel). H2O2 dan insulin juga merangsang peningkatan PPAR-γ dan IRS-1, iaitu protein-protein dalam tapakjalan transduksi insulin. Data daripada kajian ini walau bagaimanapun, menunjukkan bahawa GTT tidak memberi kesan ke atas pengambilan glukosa oleh sel. Oleh itu, kajian ini menunjukkan bahawa H2O2 bertindak seperti insulin dalam merangsang pengambilan glukosa oleh sel miotiub dengan mempengaruhi tapakjalan pengisyaratan insulin.

Kata kunci: Gama-tokotrienol; hidrogen peroksida; pengambilan glukosa; sel miotiub; tindakan insulin

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*Corresponding Author; email: musalmah@medic.ukm.my