Sains Malaysiana 39(4)(2010): 557–563

Penentuan Aktiviti Antipengoksidaan Herba Tradisi Sarawak

Kacangma Leonurus sibiricus

(Determination of Antioxidation Activities of Sarawak’s Traditional  Herb Kacangma Leonurus sibiricus)

Chua Hun Pin*

Pusat Penyelidikan Teknologi Makanan

Institut Penyelidikan dan Kemajuan Pertanian Malaysia

Stesen MARDI Kuching, Lot 411, Blok 14, Petra Jaya, 93055 Kuching, Sarawak, Malaysia

Aminah Abdullah

Program Sains Makanan, Faculty Sains dan Teknologi

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor D.E., Malaysia

Received: 14 August 2009 / Accepted: 17 November 2009

ABSTRAK

Aktiviti pengoksidaan ekstrak etanol dan air daripada kacangma (Leonurus sibiricus) telah dikaji menggunakan tiga sistem ujian. Berdasarkan turutan aktiviti antipengoksida, ekstrak etanol kacangma kering didapati tinggi dalam sistem pengoksidaan asid linoleik (LP) 71.6%, sederhana dalam sistem penindasan superoksida xantin oksidase (XOD) 69.7% dan rendah dalam sistem penindasan radikal bebas 1,2-difenil-2-pikrilhidrazil (DPPH) 48.8%. Sebaliknya, ekstrak air kacangma kering didapati tinggi dalam ketiga-tiga sistem iaitu masing-masing 72.7, 76.3 dan 78.2%. Pengekstrakan air didapati lebih berkesan dalam mengeluarkan bahan antipengoksida daripada kacangma kering berbanding pengekstrakan alkohol.

Kata kunci: Aktiviti antipengoksidaan; Leonurus sibiricus; pengekstrakan; penindasan radikal; superoksida xantin oksidase

ABSTRACT

Antioxidant activities of ethanolic and aqueous extract from kacangma (Leonurus sibiricus) were determined by using three model systems. Based on the antioxidant activity range, ethanolic extract of dried kacangma was high in oxidation of linoleic acid lipid system (LP) 71.6%, moderate in xanthine oxidase superoxide scavenging activity (XOD) 69.7% and low in 1,2-diphenyl-2-picrylhydrazyl radical scavenging activity (DPPH) 48.8%. On the other hand, aqueous extract of dried kacangma was high in antioxidant activities for all three bioassays with mean percentages of 72.7, 76.3 and 78.2%, respectively. Extraction in water was more efficient than ethanol in extracting the antioxidants present in dried kacangma.

Keywords: Antioxidation activity; extraction; Leonurus sibiricus; radical scavenging; xanthine oxidase superoxide

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*Corresponding author: hpchua@mardi.gov.my