Sains Malaysiana 41(12)(2012): 1587–1593

 

Penghasilan Hidrogen oleh Bakteria Rhodobacter Sphaeroides NCIMB 8253

(Hydrogen Production via Rhodobacter sphaeroides NCIMB 8253)

 

Nor Zawanah Ab. Hamid, Jamaliah Md Jahim*, Nurina Anuar& Sahaid Khalid

Jabatan Kejuruteraan Kimia dan Proses, Fakulti Kejuruteraan & Alam Bina

Universiti Kebangsaan Malaysia,43600 Bangi, Selangor, Malaysia

 

Received: 21 October 2011 / Accepted: 03 August 2012

 

ABSTRAK

Hidrogen merupakan sumber tenaga yang boleh diperbaharui dan efektif. Hidrogen boleh dihasilkan melalui fotofermentasi oleh bakteria ungu tanpa sulfur seperti Rhodobacter sphaeroides disebabkan kebolehan yang tinggi dalam menghasilkan hidrogen dalam keadaan anaerobik. Dalam kajian ini, bakteria dieram di dalam botol serum 100 mL dalam keadaan anaerobik. Kajian ini bertujuan untuk memaksimumkan penghasilan hidrogen oleh Rhodobacter sphaeroides NCIMB 8253 yang melibatkan kajian mengenai inokulum dalam keadaan aerobik dan anaerobik, kesan pH awal dan kesan keamatan cahaya terhadap penghasilan hidrogen. Daripada uji kaji yang dilakukan, didapati bahawa inokulum yang dieram pada keadaan anaerobik menghasilkan hidrogen tertinggi iaitu 220.5 mL/g substrat. pH 7 adalah pH yang paling sesuai digunakan untuk penghasilan hidrogen. Keamatan cahaya 5,000 lux adalah nilai optimum yang dapat memberikan penghasilan hidrogen tertinggi iaitu 80.21 mL hidrogen dengan hasil hidrogen 401.04 mL/g substrat. Penghasilan hidrogen didapati semakin menurun pada keamatan cahaya yang lebih tinggi daripada 5,000 lux.

 

Kata kunci: Bakteria fotosintetik; hidrogen, Rhodobacter sphaeroides

 

ABSTRACT

Hydrogen is a renewable source of energy and is effective. Hydrogen can be produced by photofermentation by purple non sulfur bacteria such as Rhodobacter sphaeroides, due to the high ability to produce hydrogen in anaerobic conditions. In this study, bacteria were incubated in 100 mL serum bottles in anaerobic conditions. The aims of this study were to maximize the hydrogen production of Rhodobacter sphaeroides NCIMB 8253 which involves the study of the inoculums in aerobic and anaerobic conditions and to determine the effect of initial pH and the effect of light intensity on hydrogen production. From the experiments, it was found that inoculums in anaerobic conditions produced the highest hydrogen yield 220.5 mL/g substrate. pH 7 is the most suitable pH for the hydrogen production. Light intensity of 5,000 lux is the optimum value that would give the highest hydrogen production at 80.21 mL and hydrogen yield 401.04 mL/g substrate. Hydrogen production was decline at light intensities higher than 5,000 lux.

 

Keywords: Hydrogen; photosynthetic bacteria; Rhodobacter sphaeroides

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*Corresponding author; email: jamal@eng.ukm.my