Sains Malaysiana 41(3)(2012): 333–337

Chemical Components of Marine Sponge Derived Fungus Fusarium proliferatum

Collected from Pulau Tinggi, Malaysia

(Komponen Kimia Kulat Fusarium proliferatum diperolehi daripada Span Marin

di Perairan Pulau Tinggi, Malaysia)

Z. Mosadeghzad, Z. & G. H. N. Farahani

Department of Chemistry, Faculty of Science, Islamic Azad University-Sharekord Branch

166 Shahrekord-Iran

Zuriati Zakaria

School of Chemical Sciences and Food Technology, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor D.E. Malaysia

A. Asmat, U. Gires & R. Wickneswari

School of Bio Sciences and Biotechnology, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor D.E. Malaysia

P. Pittayakhajonwut

National Centre for Genetic Engineering and Biotechnology (BIOTEC)

National Science and Technology Development Agency (NSTDA)

113, Paholyothin Road, Klong 1, Klong Luang, Pathum Thani 12120 Thailand

Received: 26 April 2011 / Accepted: 13 September 2011

ABSTRACT

Marine fungus Fusarium proliferatum derived from marine sponge collected along Pulau Tinggi, Malaysia was cultivated on Potato Dextrose Broth and incubated for 7 days at 30oC. The liquid cultures were then extracted using ethyl acetate. The crude extract was investigated for its anti-microbial activity and was passed through Sephadex column and the fractions were collected. Reverse phase HPLC was used to monitor the component of crude extract. HPLC guided purification of crude extract resulted in the isolation of linoleic acid, 4-hydroxy phenethyl alcohol, 2,5-furandimethanol and adenosine. Their structures were elucidated by spectroscopic methods.

Keywords: Antimicrobial activity; Fusarium proliferatum; marine fungi

ABSTRAK

Kulat marin Fusarium proliferatum yang diperolehi daripada span marin dikutip di perairan Pulau Tinggi, Malaysia telah dieramkan pada Agar Potato Dextrose (PDA) pada 30°C selama 7 hari. Kultur cecair diekstrak dengan pelarut etil asetat. Ekstrak mentah dikaji untuk aktiviti antimikrob dan dimasukkan ke dalam turus Sephadex dan fraksi dikumpulkan. HPLC fasa terbalik digunakan untuk memantau komponen ekstrak mentah. Penulenan ekstrak mentah berpandukan HPLC telah menghasilkan pemencilan asid linoleik, alcohol 4-hidroksifenetil, 2,5-furan dimetanol dan adenosine. Struktur telah ditentukan dengan kaedah spektroskopi.

Kata kunci: Aktiviti antimikrob; Fusarium proliferatum; kulat marin

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*Corresponding author; email: zmzad79@gmail.com