Sains Malaysiana 41(4)(2012): 403-409

Absence of Toxicity of Strobilanthes crispaJuice in Acute Oral Toxicity Study in Sprague Dawley Rats

(Ketiadaan Ketoksikan Jus Strobilanthes crispaBagi Ketoksikan Akut Secara Oral Pada Tikus Sprague Dawley)

N.A. Norfarizan-Hanoon*

School of Health Sciences, Universiti Sains Malaysia

16150 Kubang Kerian, Kelantan, Malaysia

R. Asmah & M.Y. Rokiah

Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences

Universiti Putra Malaysia, 43400 Serdang, Selangor D.E., Malaysia

O. Fauziah

Department of Human Anatomy, Faculty of Medicine and Health Sciences

Universiti Putra Malaysia, 43400 Serdang, Selangor D. E., Malaysia

H. Faridah

Malaysian Agricultural Research and Development Institute, Universiti Putra Malaysia

43400 UPM, Serdang, Selangor D.E., Malaysia

Received: 26 April 2011 / Accepted: 20 October 2011

ABSTRACT

This study evaluated four different doses of Strobilanthes crispa juice (700, 2100, 3500 and 4900 mg kg-1 of body weight) administered orally to normal female and male Sprague dawley rats on possible changes in various physical, behaviour, morphology and biochemical parameter. The rats were treated with a single dose of juice and observed for 14 days. No significant toxicity was observed with respect to clinical parameters and organ morphology. In addition, no significant changes were observed in the level of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, creatinine and albumin. The S. crispus juice was found to be safe at the maximum dose used in this study (4900 mg kg-1 of body weight).

Keywords: Acute toxicity; clinical parameters; organ morphology; S. crispus juice

ABSTRAK

Kajian ini menilai empat dos yang berbeza bagi jus Strobilanthes crispa (700, 2100, 3500 dan 4900 mg kg-1 berat badan) diberikan secara oral kepada tikus betina dan jantan Sprague Dawley ke atas perubahan dalam pelbagai fizikal, perilaku, morfologi dan parameter biokimia. Tikus diubati dengan dos tunggal jus dan diperhatikan selama 14 hari. Tidak ada ketoksikan signifikan yang didapati bagi parameter klinikal dan morfologi organ. Selain itu, tidak ada perubahan signifikan yang diamati pada tahap aspartat aminotransferase, alanin aminotransferase, alkali fosfatase, kreatinin dan albumin. Jus S. crispus didapati selamat pada dos maksimum yang digunakan dalam kajian ini (4900 mg kg-1 berat badan).

Kata kunci: Jus S. crispus; ketoksikan akut; morfologi organ; parameter klinikal

REFERENCES

Abu-Bakar, M.F., Teh, A.H., Rahmat, A., Hashim, N., Othman, F. & Fakurazi, S. 2004. Antioxidant tea from leaves of Strobilanthes crispus. Journal of Tropical Medicinal Plants 5(2): 199-204.

Asmah, R., Susi, E., Abdah, M.A., Patimah, I., Taufiq, Y.Y.H. & Mohd-Fadzelly, A.B. 2006b. Anticarcinogenic properties of Strobilanthes crispusextracts and its compounds in vitro. International Journal of Cancer Research 2(1): 47-49.

Asmah, R., Susi, E., Patimah, I., Taufiq, Y.Y.H. & Mohd-Fadzelly, A.B. 2006a. Chemical constituents, antioxidant activity and cytotoxic effects of essential oil from Strobilanthes crispus and Lawsonia inermis. Journal of Biological Sciences 6(6): 1005-1010.

Brummit, R.K. & Powell, C.E. 1992. Authors of Plants Names. Kew, Britain: Royal Botanic Gardens. Cameron, J.S. & Greger, R. 1998. Renal function and testing of function. In Oxford Textbook Clinical Nephrology, Darisou A. M. (ed.) pp. 36-39.

Christen, P. & Metzler, D. E. 1985. Aminotransferases. New York: Wiley Interscience Inc.

Coodley, E. 1970. Diagnostic Enzymology. Pennyslvania: Lea and Febiger.

Cotran, R., Kumar, V. & Robins, S. 1989. Robins Pathological Basis of Disease. 4th edition. WB Saunders Co. Harcourt.

Fauziah, O., Hanachi, P., Yogespiriya, S. & Asmah, R. 2005. Evaluation of lesion scoring and aniline hydroxylase activity in hepatocarcinogenesis rats treated with Strobilanthes crispus. Journal of Medical Sciences 5(1): 26-30.

Ismail, M., Manickam, E., Danial, M.A., Rahmat, A. & Yahaya, A. 2000. Chemical composition and antioxidant activity of Strobilanthes crispusleaf extract. Journal of Nutritional Biochemistry 11: 536-542.

Kusumoto, J. T., Shimada, I., Kakiuchi, N., Hattori, M. & Namba, T. 1992. Inhibitory effects of Indonesian plant extracts on reverse transcriptase of an RNA tumour virus (I). Phytotherapy Research 6(5): 241-244.

Mohd-Fadzelly, A.B., Arnida, H.T., Asmah, R., Fauziah, O., Normah, H. & Sharida, F. 2006a. Antiproliferative properties and antioxidant activity of various types of Strobilanthes crispustea. International Journal of Cancer Research 2(2): 152-158.

Mohd-Fadzelly, A.B., Asmah, R. & Fauziah, O. 2006b. Effect of Strobilanthes crispustea aqueous extracts on glucose and lipid profile in normal and streptozotocin-induced hyperglycaemic rats. Plant Foods for Human Nutrition 61(1): 6-11.

Moss, D.W. & Rosalki, S.B. 1996. Enzyme Tests in Diagnosis. London: Edward Arnold.

Ngaha, E.O. 1981. Renal effects of potassium dichromate in the rat: composition of urinary excretion with corresponding tissue pattern. General Pharmacology 12: 291-358.

Norfarizan-Hanoon, N.A., Asmah, R., Rokiah, M.Y., Fauziah, O. & Faridah, H. 2009a. Antihyperglycemic, hypolipidemic juice in normal and streptozotocin-induced diabetic male and female rats. International Journal of Pharmacology 5(3): 200-207.

Norfarizan-Hanoon, N.A., Asmah, R., Rokiah, M.Y., Fauziah, O. & Faridah, H. 2009b. Effects of Strobilanthes crispusjuice on wound healing and antioxidant enzymes in normal and streptozotocin-induced diabetic rats. Journal of Biological Sciences 9(7): 662-668.

Orth, S.R. & Ritz, E. 1998. The nephritic syndrome. New England Journal of Medicine 338: 1202-1211.

Rispin, A., Farrar, D., Margosches, E., Gupta, K., Stitzel, K., Carr, G., Greene, M., Meyer, W. & McCall, D. 2002. Alternative methods for the median lethal dose (LD₅₀) test: The up-and-down procedure for acute oral toxicity. ILAR Journal 43(4): 233-243.

Suherman, J., Asmah, R., Fauziah, O., Patimah, I. & NorHaslinda, A. 2004. Effect of Strobilanthes crispuson tumour marker enzymes and glutathione during chemical hepatocarcinogenesis in the rat. Pakistan Journal of Biological Sciences 7(6): 947-951.

Sunarto, P.A. 1977. Materia medica Indonesia. 1st ed. Jakarta, Indonesia: Penerbitan Directorat Jenderal Pengawasan Obat dan Makanan.

WHO-World Health Organization, 1993. Research guidelines for evaluating the safety and efficacy of herbal medicines. Report of a WHO Consultation Part 2. General Considerations in Herbal Medicine Research. World Health Organization. Regional Office for the Western Pacific. Manila.

Wurochekke, A.U., Anthony, A.E. & Obidah, W. 2008. Biochemical effects on the liver and kidney of rats administered aqueous stem bark extract of Xemenia americana. African Journal of Biotechnology 7(16): 2777-2780.

*Corresponding author; email: nhanoon@kk.usm.my