Sains Malaysiana 41(5)(2012): 539–545

Antioxidant Activity of Ardisia crispa (Mata pelanduk)

(Aktiviti Antioksida daripada Ardisia crispa (Mata pelanduk))

Hassan Mahmood Kzar Jindal & Jamaludin Mohamad*

Institute of Biological Sciences, Faculty of Science

University of Malaya, 50603 Kuala Lumpur, Malaysia

Received: 11 April 2011/Accepted: 11 November 2011

ABSTRACT

The leaf and fruit crude extracts of hexane, chloroform, methanol and water of Ardisia crispa were screened for their antioxidant activity using DPPH radical scavenging, ferric reducing power, and metal chelating antioxidant assay. The methanol crude extract of fruits showed higher antioxidant activity (90.16 ± 0.01%) than the methanol crude extract of leaves (82.24 ± 0.02%) in the DPPH radical scavenging assay. In the ferric reducing power assay methanol fruit extract showed the highest absorbance indicating high antioxidant activities than leaf extract. In the metal chelating antioxidant assay fruit methanol extract gave 40% antioxidant activities than the leaf. Thin Layer Chromatography of the fruit methanol crude extract showed that it contained phenolic compounds when it was detected with folin reagent. HPLC analysis revealed that the fruit methanol extract contained gallic acid. This indicated that the high antioxidant activities of the fruits were due to the presence of gallic acid in the fruits of Ardisia crispa.

Keywords: Antioxidants; DPPH radical scavenging activity; gallic acid; metal chelating activity; reducing power assay

ABSTRAK

Ekstrak mentah heksan, kloroform, metanol, air daripada daun dan buah Ardisia crispa telah disaring untuk menentukan aktiviti antioksidan menggunakan asai antioksidan penyahbebas radikal DPPH, kuasa penurunan ferik, dan logam pengkelat. Ekstrak metanol buah menunjukkan aktiviti antioksidan yang lebih tinggi daripada ekstrak daun dengan peratus perencatan penyahbebas DPPH 90.16 ± 0.01% and 82.24 ± 0.02% masing-masing. Dalam asai kuasa penurunan ferik ekstrak metanol buah menunjukkan serapan yang tertinggi menandakan aktiviti antioksidan yang tinggi daripada ekstrak daun. Dalam asai logam pengkelat ekstrak metanol, buah memberikan 40% aktiviti antioksidan daripada daun. Kromatografi lapisan nipis ekstrak mentah buah menunjukkan, terdapat kandungan sebatioan fenolik. Analisis HPLC menunjukkan akstrak mentah mengandungi asid galik. Ini menunjukan aktiviti antioksidan yang tinggi di dalam buah adalah disebabkan kehadiran asid galik dalam buah Ardisia crispa.

Kata kunci: Aktiviti pengkelat logam; antioksidan; asai kuasa penurun; asid galik; penyahbebas radikal DPPH

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*Corresponding authors; email: jamal@um.edu.my