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Sains Malaysiana 42(11)(2013):
1599–1603
Gene Mutation Effect of Aqueous and Methanol Extracts of Salted Fish from Pulau Pinang, Malaysia towards V79 Lung Fibroblast Cells (Kesan Mutasi Gen Ekstrak Akues dan Metanol Ikan Masin dari Pulau Pinang, Malaysia terhadap
Sel FibroblasParu-paru V79) Ahmad Rohi Ghazali*, Rasyidah Mohamad Halim, Nor Fadilah Rajab, Noorain Ramli, Rozaini Abdullah, Firdaus Kamarulzaman & Zaliha Harun
Biomedical Science Programme,
School of Diagnostic and Applied Health Sciences
Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz
50300 Kuala Lumpur, Malaysia
Received: 2 February 2012/Accepted: 9 April 2013
ABSTRACT
Salted fish is a locally processed raw food which is used in
everyday cooking among Malaysians. Previous studies suggested that salted fish
intake was a risk of nasopharyngeal cancer. Hence, this study was carried out
to evaluate gene mutation effects through the induction of mutagenic effect of
aqueous and methanol extracts of salted fish from Balik Pulau, Pulau Pinang,
Malaysia. Balik Pulau was
chosen for sampling purpose due to its popularity as a commercial centre for local raw fisheries in Malaysia. Evaluation of
mutagenic effect was carried out by hprt Gene Mutation Assay towards V79 lung fibroblast cells. It was
found that the aqueous and methanol extracts of salted fish were not cytotoxic
towards V79 lung fibroblast cells. It was also found that the extracts of
salted fish from Balik Pulau were not mutagenic towards hprt gene of V79
lung fibroblast cells as the mutation frequency of the extracts did not exceed
3 times of the value for negative control mutation frequency. In conclusion,
both aqueous and methanol extracts of salted fish from Balik Pulau did not have gene mutation effect towards hprt gene in vitro. However, other
toxicological profile could be assessed to determine the mechanism of toxicity
of salted fish.
Keywords: Cytotoxic; gene mutation; hprt; Pulau Pinang; salted fish
ABSTRAK
Ikan masin merupakan salah satu makanan tempatan
terproses yang kerap
digunakan dalam masakan harian penduduk Malaysia. Kajian terdahulu menunjukkan
perkaitan antara
pengambilan ikan masin dengan risiko
menghidap penyakit
kanser nasofarinks. Oleh itu, kajian ini
telah dijalankan
untuk menilai kesan
mutasi gen melalui
induksi aktiviti mutagenisiti pada ekstrak akues dan
metanol ikan
masin dari Balik
Pulau, Pulau
Pinang, Malaysia. Balik Pulau telah
dipilih sebagai kawasan persampelan kerana popularitinya sebagai pusat pengeluaran
ikan mentah
tempatan di Malaysia. Penilaian kesan
mutagenik telah
dijalankan dengan menggunakan Asai Mutasi Gen hprt menggunakan sel selanjar V79 iaitu sel fibroblas paru-paru. Kajian ini mendapati
bahawa ekstrak akues dan metanol
ikan masin
tidak sitotoksik terhadap sel fibroblas paru-paru V79. Hasil
kajian ini juga
mendapati bahawa
ekstrak ikan masin
dari Balik
Pulau tidak mutagenik
ke arah
gen hprt sel fibroblast
paru-paru V79 dengan
frekuensi mutasi
ekstrak tidak melebihi
3 kali nilai frekuensi
mutasi berbanding kawalan negatif. Kesimpulannya,
kedua-dua ekstrak akues dan metanol
ikan masin
dari Balik Pulau
tidak menyebabkan
mutasi pada gen hprt secara in vitro.
Kata kunci: hprt; ikan masin; mutasi gen; Pulau Pinang; sitotoksisiti
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