Sains Malaysiana 42(11)(2013): 1613–1618

Single Nucleotide Polymorphism for Certain Genes Involved in Gestational Diabetes

with Risk Factors and Complications Positive

(Polimorfisme Nukleotida Tunggal bagi Gen Tertentu yang Terlibat dalam Faktor Risiko

dan Komplikasi Positif Diabetes Gestasi)

NOR AZLIN MOHAMED ISMAIL¹*, NORKHATIJAH MOHD ARIS¹, ZALEHA ABDULLAH MAHDY¹, SHUHAILA AHMAD¹, NORZILAWATI MOHD NAIM¹, HARLINA HARLIZAH SIRAJ¹, ROHANA JAAFAR², SHAREENA ISHAK², ROSLAN HARUN³, ABD RAHMAN ABD JAMAL³, WAN ZURINAH WAN NGAH³& SYED ZULKIFLI SYED ZAKARIA³

¹Department of Obstetrics & Gynaecology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latiff

56000 Cheras, Kuala Lumpur, Malaysia

²Department of Paediatrics, Faculty of Medicine

Universiti Kebangsaan Malaysia, Jalan Yaacob Latiff, 56000 Cheras, Kuala Lumpur, Malaysia

  ³UKM Medical Molecular Biology Institute, Faculty of Medicine

Universiti Kebangsaan Malaysia, Jalan Yaacob Latiff, 56000 Cheras, Kuala Lumpur, Malaysia

Received: 19 July 2012/Accepted: 9 April 2013

ABSTRACT

Gestational Diabetes Mellitus (GDM) is associated with pregnancy complications, however its mechanism has not been fully understood. The aim of this study was to investigate the single nucleotide polymorphism (SNP) for identifying candidate genes involve in risk factors and complications of GDM. A total of 174 pregnant women with GDM and 114 healthy pregnant women were genotyped with 384 SNPs from 236 genes. The SNPs identified were rs10946398 (CDKAL1) in GDM risk factors; rs328 (LPL) and rs1042778 (OXTR) in complications of caesarean section; rs5404 (SLC2A2), rs5400 (SLC2A2) and rs13306465 (IRS1) for neonatal intensive care admission. Whereby SNPs rs12255372, rs7901695 and rs7903146 from TCF7L2 gene had six times higher risk (OR, 6.40-6.53) for T2DM at postpartum. In conclusion, although the above SNPs were identified with GDM risk factors and complications among pregnant Malaysian women with GDM, a larger study is needed to ascertain this candidate genes actual association.

Keywords: Complications; gestational diabetes mellitus; single nucleotide polymorphisms; risks; Malaysia

ABSTRAK

Diabetes Melitus Gestasi (GDM) mempunyai perkaitan dengan komplikasi kehamilan, namun demikian mekanismenya masih belum dikenal pasti sepenuhnya. Kajian ini adalah untuk mengkaji polimorfisme nukleotida tunggal (SNP) bagi mengenal pasti gen yang terlibat dalam faktor risiko GDM dan komplikasinya. Sejumlah 174 wanita hamil dengan GDM and 114 wanita hamil yang sihat telah digenotip dengan 384 SNPs daripada 236 gen. SNPs yang dikenal pasti adalah rs10946398 (CDKAL1) dalam faktor risiko GDM; rs328 (LPL) dan rs1042778 (OXTR) dalam komplikasi pembedahan caesarean; rs5404 (SLC2A2), rs5400 (SLC2A2) dan rs13306465 (IRS1) untuk kemasukan wad rawatan rapi neonatal. Sementara SNPs rs12255372, rs7901695 dan rs7903146 daripada gen TCF7L2 mempunyai enam kali lebih tinggi risiko (OR, 6.40-6.53) untuk T2DM semasa postpartum. Sebagai kesimpulan, walaupun SNPs di atas telah dikenal pasti dengan faktor risiko GDM dan komplikasinya dalam kalangan wanita Malaysia yang hamil dengan Diabetes Gestasi, satu kajian yang lebih besar adalah perlu bagi memastikan penglibatan sebenar gen tersebut.

Kata kunci: Diabetes melitus gestasi; komplikasi; polimorfisme nukleotida tunggal; risiko; Malaysia

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*Corresponding author; email: azlinm@ppukm.ukm.edu.my