Sains Malaysiana 42(1)(2013): 99–105

Lactate Dehydrogenase Activity During Tooth Movement under 1.0 N

and 1.5 N Continuous Force Applications

(Aktiviti Laktat Dehidrogenase Semasa Pergerakan Gigi dengan Aplikasi

Tekanan 1.0 N dan 1.5 N Secara Berterusan)

Shahrul Hisham Zainal Ariffin & Nurfathiha Abu Kasim

School of Bioscience and Biotechnology, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor D.E. Malaysia

Rohaya Megat Abdul Wahab*

Department of Orthodontics, Faculty of Dentistry, Universiti Kebangsaan Malaysia

50300 Kuala Lumpur, Malaysia

Abdul Aziz Jemain

DELTA, School of Mathematics, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor D.E.Malaysia

Received: 19 December 2011 / Accepted: 10 July 2012

ABSTRACT

The aim of this study was to observe the pattern of lactate dehydrogenase (LDH) activity in GCF and the rate of tooth movement at two different orthodontic forces (1.0 N and 1.5 N). Twelve subjects participated in this study and was chosen based on the inclusion criteria. Each subject received forces of 1.0 N and 1.5 N for tooth movement either on the left or right side of the maxillary canine. GCF sample was collected at mesial and distal sites of the canines before applying the appliance (week 0) and every week for 5 weeks after tooth movement (week 1 to week 5) where baseline activity served as control. LDH activity was assayed spectrophotometically at 340 nm. The tooth movements were measured from casted study models. LDH specific activity at mesial sites in 1.0 N and 1.5 N force groups, respectively increased significantly (p<0.05) only on week four and throughout the treatment when compared with baseline. At distal sites, LDH specific activity with 1.5 N was higher than 1.0 N throughout the five weeks of tooth movement. LDH specific activity with 1.5 N force increased at both mesial (week 2) and distal sites (week 3) with significant different (p<0.05) when compared with 1.0 N force. Tooth movement with 1.5 N showed significantly faster (p<0.05) at the end of week 5 when compared with 1.0 N. LDH has the potential as a biological marker of inflammation during tooth movement.A force of 1 N was more suitable to be used although less tooth movement was produced because less inflammation caused by the force can be useful in orthodontic treatment for patients with stabilised periodontal diseases compared with 1.5 N force.

Keywords: Biological marker; inflammation; lactate dehydrogenase; orthodontic force; tooth movement

ABSTRAK

Kajian ini bertujuan untuk melihat corak aktiviti laktat dehidrogenase (LDH) di dalam GCF dan kadar pergerakan gigi pada dua daya tekanan ortodontik yang berbeza (1.0 N dan 1.5 N). Dua belas orang subjek telah mengambil bahagian dalam kajian ini dan mereka dipilih berdasarkan beberapa kriteria yang telah ditetapkan. Setiap subjek menerima 1.0 N dan 1.5 N daya tekanan untuk pergerakan gigi sama ada pada bahagian kanan atau kiri gigi taring maksila. Sampel GCF dikumpul dari bahagian mesial dan distal gigi taring sebelum dipakaikan pendakap gigi (minggu 0) dan setiap minggu untuk lima minggu selepas gigi digerakkan (minggu 1 hingga minggu 5) dengan aktiviti basal dijadikan sebagai kawalan. Aktiviti LDH diasai menggunakan pendekatan spektrofotometri pada 340 nm. Pergerakan gigi diukur daripada model-model kajian yang telah dibentuk. Aktiviti spesifik LDH pada bahagian mesial dalam kumpulan tekanan 1.0 N dan 1.5 N masing-masing meningkat secara signifikan (p<0.05) hanya pada minggu 4 dan sepanjang rawatan berbanding kawalan. Pada bahagian distal, aktiviti spesifik LDH dengan 1.5 N adalah lebih tinggi berbanding 1.0 N sepanjang lima minggu pergerakan gigi. Aktiviti spesifik LDH dengan tekanan 1.5 N meningkat (p<0.05) pada kedua-dua bahagian mesial (minggu 2) dan distal (minggu 3) berbanding tekanan 1.0 N. Pergerakan gigi dengan 1.5 N lebih pantas (p<0.05) pada akhir minggu 5 berbanding dengan 1.0 N. LDH berpotensi sebagai penanda biologi untuk inflamasi semasa pergerakan gigi. Daya tekanan 1.0 N berbanding 1.5 N lebih sesuai digunakan walaupun ia menghasilkan kurang pergerakan gigi kerana penghasilan inflamasi yang rendah adalah penting dalam rawatan ortodontik kepada pesakit periodontal yang telah stabil.

Kata kunci: Inflamasi; laktat dehidrogenase; penanda biologi; pergerakan gigi; tekanan ortodontik

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*Corresponding author; email: shahroy7@gmail.com