Sains Malaysiana 42(2)(2013): 123–127

 

Total Phenolics and Antioxidant Activities of Pouteria campechianaFruit Parts

(Fenolik Jumlah dan Aktiviti Antioksida Sebatian Bahagian Buah Pouteria campechiana)

 

 

K.W. Kong¹, H.E. Khoo², N.K. Prasad³, L.Y. Chew⁴, I. Amin^2,5*

 

¹Department of Molecular Medicine, Faculty of Medicine, University of Malaya

50603 Kuala Lumpur, Malaysia

 

²Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences

Universiti Putra Malaysia, UPM Serdang, 43400 Selangor, Malaysia

 

³Monash University, Chemical Engineering Discipline, School of Engineering

Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor, Malaysia

 

⁴School of Biosciences, Taylor’s University, Taylor’s Lakeside Campus

No. 1, Jalan Taylor’s, 47500 Subang Jaya, Selangor, Malaysia

 

⁵Laboratory of Halal Science Research, Halal Products Research Institute

Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

 

 

Received: 26 April 2012 / Accepted: 10 October 2012

 

ABSTRACT

This study aimed to evaluate the total phenolics and antioxidant capacities of the seeds, pulp and peel of Pouteria campechiana fruit using three extraction solvents (water, 70% methanol and 70% ethanol). Among them, 70% ethanol exhibited the best solvent for yielding highest total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activities. The result showed that 70% ethanol extract from the peel contained the highest TPC (2304.7 mg gallic acid equivalent/100 g dw) while the pulp has the highest TFC (6414.03 mg rutin equivalent/100 g dw). The antioxidant activities of the pulp and peel ethanolic extracts were high as determined using 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation assay (49.60 and 49.56 mmoL TE/100 g dw) and ferric reducing antioxidant power assay (43.88 and 42.94 Fe2+/100 g dw) but not for seeds. However, their diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities were ~88%. Thus, the pulp and peel of P. campechiana fruit can be utilized as natural source for antioxidant components.

 

Keywords: Antioxidant activity; Pouteria campechiana; solvent extraction; total phenolics

 

ABSTRAK

Kajian ini bertujuan untuk menentukan fenolik jumlah dan keupayaan antioksidan daripada biji, isi dan kulit buahPouteria campechiana dengan menggunakan tiga pelarut pengekstrakan (air, 70% metanol dan 70% etanol). Antara pelarut tersebut, 70% etanol adalah yang terbaik untuk mendapatkan kandungan fenolik jumlah (TPC), kandungan flavonoid jumlah (TFC) dan mempunyai aktiviti antioksidan yang tertinggi. Keputusan kajian menunjukkan bahawa ekstrak 70% etanol daripada kulit buah mengandungi TPC tertinggi (2304.7 mg persamaan asid galik /100 g bk), manakala isinya mengandungi TFC yang tertinggi (6414.03 mg persamaan rutin /100 g bk). Aktiviti antioksidan ekstrak etanol isi dan kulit yang ditentukan oleh asai radikal kation ABTS (49.60 and 49.56 mmoL TE/100 g bk) dan asai kuasa antioksidan penurunan ferik (43.88 and 42.94 Fe2+/100 g bk) adalah tinggi tetapi tidak untuk bijinya. Aktiviti merantas radikal DPPH adalah ~88%. Maka, isi dan kulit buah P. campechiana boleh digunakan sebagai sumber semula jadi untuk mendapatkan sebatian antioksidan.

 

Kata kunci: Aktiviti antioksidan; jumlah fenolik; pelarut pengestrakan; Pouteria campechiana

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*Corresponding author; email: amin@medic.upm.edu.my