Sains Malaysiana 42(9)(2013): 1267–1272

In vitro Antifungal Activities and Phytochemical Analysis of Filamentous

White-rot Fungi, Schizophyllum commune

(Aktiviti Anti-kulat Pereput In vitro dan Analisis Fitokimia bagi Kulat Pereput

Putih Berfilamen, Schizophyllum commune)

Yi Peng Teoh & Mashitah Mat Don*

School of Chemical Engineering, Universiti Sains Malaysia, 14300 Nibong Tebal

Seberang Perai South, Penang, Malaysia

Received: 23 July 2012/ Accepted: 16 December 2012

ABSTRACT

In this study, the in-vitro antifungal activity and phytochemical analysis of Schizophyllum commune extracts have been investigated. The antifungal activity was tested against 11 species of selected wood degrading fungi of rubberwood. The results showed that water, methanol and ethanol extracts significantly inhibited the growth of wood decaying fungi with minimum inhibitory concentration (MIC) ranges 0.16-5.00 μg/μL. P. sanguineus was found as the strongest wood degrading fungus where it required the highest concentration of S. commune crude extracts (≥ 5.00 μg/μL) to inhibit its mycelia growth.  Phytochemicals analysis revealed that the extracts contained flavonoid, phenol and saponin. The methanol extracts of S. commune was then applied on the rubberwood blocks and found that the growth of P. sanguineus was inhibited effectively at 5.00 μg/μL.

Keywords: Antifungal activity; minimum inhibitory concentration (MIC); rubberwood block; Schizophyllum commune; wood decaying fungi

ABSTRAK

Dalam kajian ini, aktiviti anti-kulat in vitro dan analisis fitokimia bagi kulat Schizophlyllum commune telah dikaji. Aktiviti anti-kulat telah diuji ke atas 11 spesies kulat pereput putih kayu yang terpilih. Keputusan menunjukkan ekstrak air, metanol dan etanol boleh merencat pertumbuhan kulat pereput putih kayu dengan kepekatan rencatan minimum (KMM) di dalam julat 0.16-5.00 μg/μL. P. sanguineus telah dikenal pasti sebagai kulat pereput putih kayu yang terkuat kerana ia memerlukan kepekatan ekstrak kulat S. commune yang tinggi (≥5.00 μg/μL) untuk merencat pertumbuhan misilianya. Analisis fitokimia pula menunjukkan yang ekstrak ini mengandungi flavanoid, asid karbolik dan saponin. Ekstrak methanol daripada kulat S. commune ini juga telah diuji keberkesanannya ke atas blok kayu getah dan didapati kulat P. sanguineus telah terencat secara efektif pada 5.00 μg/μL.

Kata kunci: Aktiviti antikulat; blok kayu getah; kepekatan merencat minimum (KMM); kulat pereput kayu; Schizophyllum commune

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*Corresponding author; email: chmashitah@eng.usm.my