Sains Malaysiana 43(12)(2014): 1889–1893

Effects of Salvadora persicaExtract on the Bacterial Population in Single-species Biofilm

(Kesan Ekstrak Salvadora persica terhadap Populasi Bakteria dalam Biofilem Spesies-tunggal)

NORDIN FATIN-MAJDINA¹, HAJI ABD RAHIM ZUBAIDAH¹*, ABD RAZAK MONICA@MUNIRAH² & MOHD BAKRI MARINA¹

¹Department of Oral Biology, Faculty of Dentistry, University of Malaya

Lembah Pantai, 50603 Kuala Lumpur, Malaysia

²Jabatan Al-Quran dan Hadith, Akademi Pengajian Islam, University of Malaya

Lembah Pantai, 50603 Kuala Lumpur, Malaysia

Received: 30 June 2013/Accepted: 25 April 2014

ABSTRACT

The effect of S. persica extract on the population of bacteria in single-species biofilm was investigated. An artificial mouth (NAM) system was used for the development of biofilm. In one experiment, aqueous extract of S. persica at sub-MIC concentration (5 mg/mL) was first pumped into the NAM system to treat the experimental pellicle on glass beads before inoculating it with the bacteria (Strep. mitis, Strep. mutans and Strep. sanguinis). This would resemble using the aqueous extract of S. persica before meals. In another experiment, the aqueous extract of S. persica was pumped into the NAM system after the bacterial inoculation and this would resemble using it after meals. In both experiments, 24 h biofilms were allowed to form. The bacterial population of the biofilms was determined and expressed as colony forming unit per mL (cfu mL^-1). For negative control, sterilized distilled water was used whereas for positive control, commercial Listerine. It was found that the population of Strep. mitis, Strep. mutans and Strep. sanguinis in the respective biofilms for both experiments involving treatment with S. persica extract or Listerine was significantly reduced by more than 70% (p<0.05) when compared with the negative control. Comparing the effect of S. persica with Listerine on the bacterial population of the biofilms when used either before or after meal showed that S. persica is slightly less effective towards S. sanguinis before meal and towards S. mutans after meal (p<0.05). This study suggests that the efficiency of the antibacterial effect of S. persica is species-related and by its way of usage.

Keywords: Bacterial population; oral biofilm; single-species biofilm; S. persica

ABSTRAK

Kesan ekstrakS. persica terhadap populasi bakteria dalam biofilem spesis-tunggal dikaji. Model mulut tiruan (NAM) digunakan dalam proses perkembangan biofilem ini. Dalam kajian pertama, ekstrak akueusS. persica pada kepekatan separuh MIC (5 mg/mL) dipam masuk ke dalam sistem NAM bagi merawat pelikel eksperimen yang terbentuk pada manik kaca sebelum diinokulasi dengan bakteria terpilih (Strep. mitis, Strep. mutans dan Strep. sanguinis). Langkah ini menyerupai penggunaan ekstrak akueusS. persica sebelum makan. Dalam kajian kedua, ekstrak akueusS. persica dipam masuk ke dalam sistem NAM selepas inokulasi bakteria terpilih dan langkah ini menyerupai penggunaan ekstrak selepas makan. Pembentukan biofilem dalam kedua-dua kajian dibiarkan selama 24 jam. Populasi bakteria yang terbentuk pada biofilem dikenal pasti dan dibentangkan dalam unit koloni per mL (cfu mL^-1). Air suling steril digunakan sebagai kawalan negatif dan Listerine komersial sebagai kawalan positif. Keputusan bagi kedua-dua kajian menunjukkan Strep. mitis, Strep. mutans dan Strep. sanguinis dalam biofilem masing-masing melibatkan rawatan dengan ekstrakS.persica atau Listerine dibandingkan dengan kawalan negatif berupaya mengurangkan perlekatan bakteria secara signifikan sehingga lebih daripada 70% (p<0.05). Perbandingan kesanS. persica dengan Listerine terhadap populasi bakteria dalam biofilem yang digunakan sebelum dan selepas makan menunjukkan sedikit perbezaan keberkesananS. persica terhadap Strep. sanguinis sebelum makan danStrep. mutans selepas makan (p<0.05). Kajian ini menunjukkan keberkesanan kesan antibakteriaS. persica adalah berkait dengan spesies dan cara penggunaannya.

Kata kunci: Biofilem mulut; biofilem spesies-tunggal; populasi bakteria; S. persica

REFERENCES

Addy, M., Slayne, M.A. & Wade. W.G. 1992. The formation and control of dental plaque-an overview. Journal of Applied Bacteriology 73: 269-278.

Al-Bayati, Firas, A. & Khudir D. Sulaiman. 2008. In vitro antimicrobial activity of Salvadora persicaL. extracts against some isolated oral pathogens in Iraq. Turkish Journal of Biology 32: 57-62.

Al-Bayaty, F.H, Aiman Hamad Al-Koubaisi, Nidhal Abdul Wahid Ali & Mahmood Ameen Abdulla. 2010. Effect of mouth wash extracted form Salvadora persica(Miswak) on dental plaque formation: A clinical trail. Journal of Medicinal Plants Research 4(14): 1446-1454.

Al-Mas, Khalid. 2001. The antimicrobial effects of seven different types of Asia chewing sticks. Odonto-Stomalogie Tropicale Journal 24(96): 17-20.

Al-Mas, Khalid & Zuhair Al-Zeid. 2004. The immediate antimicrobial effect of a toothbrush and Miswak on cariogenic bacteria: A clinical study. The Journal of Contemporary Dental Practice 5(1): 1-9.

Bos, G. 1993. The Miswak, an aspect of dental care in Islam. Medical History 37: 68-79

Brecx, M., Netuschil, M., Reichert, B. & Schreil, G. 1990. Efficacy of listerine, meridol and chlorhexidine mouthrinses on plaque, gingivitis and plaque bacteria vitality. Journal of Clinical Periodontol5: 292-297.

Ezoddini-Ardakani, Fatemeh. 2010. Efficacy of Miswak (Salvadora persica) in preventing dental caries. Health 2(5): 499-503.

Fathilah, A.R. 2011. Piper betle L. and Psidium guajava L. in oral health maiantance. Journal of Medicinal Plants Research 5(2): 156-163.

Goyal, Manoj, Sasmal, D. & Nagori. B.P. 2011. Salvadora persica (Meswak): Chewing stick for complete oral care. International Journal of Pharmacology 7(4): 440-445.

Halawany, Hassan Suliman. 2012. A review on miswak (Salvadora persica) and its effect on various aspects of oral health. The Saudi Dental Journal 24(2): 63-69.

Hasnor, W.I., Wan Nordini, A.R., Fathilah, M., Md. Yusoff & Rahim. Z.H.A. 2008. The behaviour of selected oral bacteria in single-and mixed-species biofilms. Journal of Biological Sciences 8(6): 1001-1007.

Ma'ayergi, H.A., Ismail, S.I., Batanouny, K.H. & Rizk, A.M. 1984. Ecological and phytochemical studies on the Miswak, Salvadora persicaL. Qatar University Science Bulletin 4: 37-44.

Nield-Gehrig, Jill S. 2003. Dental Plaque Biofilms. http://www. dentalcarestamford.com/pdf/Denta%20Plaque%20Biofilms. pdf. Accessed on 29 January 2013.

Prashant, G.M., Chandu, G.N., Murulikrishna, K.S. & Shafiulla, M.D. 2007. The effect of mango and neem extract on four organisms causing dental caries: Streptococcus mutans, Streptococcus salivavius, Streptococcus mitis, and Streptococcus sanguis: An in vitro study. Indian Journal of Dental Research 18(4): 148-151.

Rahim, Z.H.A., Fathilah, A.R., Irwan, S. & Wan Nordini Hasnor. W.I. 2008. An artificial mouth system (NAM Model) for oral biofilm research. Research Journal of Microbiology 3(6): 466-473.

Shapiro, S, Meier, A. & Guggenheim, B. 1994. The antimicrobial activity of essential oils and essential oil components towards oral bacteria. Oral Microbiol. Immunol. 9: 202-208.

Shapiro, S. & Guggenheim, B. 1995. The action of thymol on oral bacteria. Oral Microbiol. Immunol. 10(4): 241-246.

Sofrata, A.H. 2010. Salvadora persica(Miswak): An Effective Way of Killing Oral Pathogens. Periodontology Department, Faculty of Dentistry, Karolinska Institute, Stockholm, Sweden.

*Corresponding author; email: zubaidar@um.edu.my