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Sains Malaysiana 43(4)(2014): 559–566
Kesan
Apigenin, Berberin dan Rutin terhadap Metabolisme Kolesterol pada
Sel Kanser Hep G2 (Effect of Apigenin, Berberine
and Rutin on Cholesterol Metabolism in Hep G2 Cancer Cell)
MOHD KAMAL
NIK
HASSAN1*, RASADAH
MAT
ALI1, ZULKHAIRI
HJ.
AMOM2, MOHD
SHAHIDAN
MOHD
ARSHAD1, ZAMREE MD SHAH1, KHAIRUL
KAMILAH
ABDUL
KADIR1 & IHSAN
SAFWAN
KAMARAZAMAN1
1Bahagian
Hasilan Semula Jadi, Pusat Penyelidikan Perhutanan Malaysia, 52109
Kepong, Kuala Lumpur Malaysia
2Fakulti
Sains Kesihatan, UiTM Kampus Puncak Alam, 42300 Puncak Alam, Selangor
Malaysia
Received: 12
March 2012/Accepted: 31 July 2013
ABSTRAK
Dalam kajian ini, keupayaan Apigenin, Berberin dan Rutin untuk
mengurangkan metabolisme
kolesterol pada sel kanser hepatoma
manusia (Hep
G2) telah ditentukan. Penilaian sitotoksik Apigenin, Berberin dan Rutin telah
dilakukan dengan
mendedahkan Hep G2 kepada Apigenin, Berberin dan Rutin
pada kepekatan
antara 7.8 sehingga 1000 μg/mL
selama 24 jam pada
suhu 37oC dan
5% atmosfera CO2.
Apigenin, Berberin
dan Rutin masing-masing
mempunyai kepekatan
perencat 20 (IC20) 7.8, 125 dan 1000 μg/mL. Keupayaan mengurangkan metabolisme kolesterol oleh Apigenin, Berberin dan Rutin
pada Hep
G2 telah diuji dengan
penyemaian Hep
G2 dalam plat 6-telaga. Kumpulan rawatan
Apigenin, Berberin
dan Rutin telah
dirawat dengan
kepekatan 7.8, 31.25 dan 62.5
μg/mL masing-masing dan
didedah dengan
lipoprotein ketumpatan rendah (LDL)
sebanyak 10 μL.
Dalam kumpulan kawalan normal (NC), Hep
G2 telah dieram
dengan media kultur sahaja. Sel diinkubasikan dan media telah diambil untuk
analisis Apo A1, LCAT,
LDLR
dan FDFT1 dengan menggunakan kit. Rutin didapati mampu merendahkan aktiviti HMGR secara
signifikan (p<0.05) berbanding kawalan normal. Apigenin dan Berberin mampu
meningkatkan kepekatan
APO
A1. Ketiga – tiga
sampel mampu meningkatkan
kepekatan LCAT pada
sel yang dirawat.
Selain itu, Apigenin
mampu meningkatkan
kepekatan LDLR. Keputusan
ujian untuk
FDFT1 menunjukkan Berberin dan Rutin merendahkan
kepekatan FDFT1 dan
berbeza secara
signifikan (p<0.05) berbanding
kawalan normal. Penemuan
ini menunjukkan bahawa Apigenin, Berberin dan Rutin
mempunyai potensi
dalam mengurangkan metabolisme kolesterol dalam sel Hep
G2. Kata kunci: Apigenin;
Berberin; metabolisme
kolesterol; Rutin
ABSTRACT
In this study, the ability of Apigenin,
Berberine and Rutin to reduce
cholesterol metabolism in human hepatoma
cancer cell line (Hep G2) was determined.
Cytotoxic assessment of Apigenin, Berberine
and Rutin were performed by exposing the Hep
G2 to Apigenin, Berberine
and Rutin at concentrations ranging
from 7.8 to 1000 μg/mL for 24 h at 37oC and with 5% CO2 atmosphere.
The inhibition concentration 20 (IC20) of Apigenin, Berberine and Rutin were 7.8, 125 and 1000 μg/mL, respectively. The
ability of reducing cholesterol metabolism effects of Apigenin,
Berberine and Rutin
on Hep G2 was carried out by seeding the cell in 6-well plates.
In the treated groups, Hep G2 was treated
with Apigenin, Berberine
and Rutin (7.8, 31.25 and 62.5 μg/mL,
respectively) and exposed to 10 μL
low density lipoprotein (LDL). In the normal control (NC)
groups, Hep G2 was incubated with culture
medium only. The cell was incubated and media was taken for analysis
of Apo A1, LCAT,
LDLR
and FDFT1 by using kit. Rutin was able to lower down HMGR activity
significantly (p<0.05) compared with
normal control. Apigenin and Berberin
were able to increase the concentration of APO A1.
All the three samples can increase the concentration of LCAT in
treated cells. In addition, Apigenin
can increase the concentration of LDLR. The test results for FDFT1
showed that Berberin and
Rutin can lower down FDFT1 concentrations and significantly
different (p<0.05) compared with normal controls. These
findings suggested that Apigenin, Berberine
and Rutin have the potential in reducing
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*Corresponding author; email: mohdkamal@frim. gov.my
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