Sains Malaysiana 43(7)(2014): 1031–1036

Ameliorative Effect of Palm Oil Tocotrienol Rich Fraction on Brain Oxidative Stress

in Fenitrothion-administered Rats

(Kesan Pemulihan Pecahan Kaya Tokotrienol Minyak Kelapa Sawit terhadap Tekanan Oksidatif Otak Tikus Diadministrasi Fenitrotion)

SITI BALKIS BUDIN*, IZATUS SHIMA TAIB, PUTRI AYU JAYUSMAN, HUI HUI CHIANG, ANAND RAMALINGAM, AHMAD ROHI GHAZALI & JAMALUDIN MOHAMED

Program of Biomedical Science, School of Diagnostic and Applied Health Sciences

Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz

50300 Kuala Lumpur, Malaysia

Received: 6 December 2012/Accepted: 27 September 2013

ABSTRACT

Fenitrothion (FNT) usage has received much attention for its potential to promote free radicals generation and interfere with antioxidant defense system. The aim of the present study was to investigate the effect of palm oil tocotrienol rich fraction (TRF) supplementation on oxidative stress and histological changes in rat brain induced by FNT. A total of 32 male Sprague Dawley rats divided into four groups: control group which received corn oil; TRF group was received palm oil TRF (200 mg/kg bw); FNT group administered with FNT (20 mg/kg bw) and TRF+FNT group pretreated with palm oil TRF (200 mg/kg bw) 30 min prior to administration of FNT (20 mg/kg bw). FNT and TRF were dissolved in corn oil and all supplementations were given by oral gavage once daily for 28 days. After four weeks of supplementation, TRF+FNT rats had significantly lower malondialdehyde (MDA) content and superoxide dismutase (SOD) activity but higher reduced glutathione (GSH) level and total protein level compared to FNT rats (p<0.05). However, protein carbonyl (PC) level was insignificantly lower for TRF+FNT group compared to FNT group. In conclusion, this study suggested that palm oil TRF was effective in preventing brain damage in rats.

Keywords: Fenitrothion; oxidative damage; palm oil tocotrienol rich fraction

ABSTRAK

Kegunaan fenitrothion (FNT) telah mendapat perhatian ramai kerana insektisida ini berupaya untuk mengaruh penjanaan radikal bebas dan menganggu sistem pertahanan antioksidan. Tujuan kajian ini adalah untuk mengetahui kesan pecahan kaya tokotrienol (TRF) minyak sawit terhadap tekanan oksidatif dan perubahan histologi otak tikus teraruh FNT. Tiga puluh dua ekor tikusSprague Dawley jantan telah dibahagikan kepada empat kumpulan: kumpulan kawalan yang menerima minyak jagung; kumpulan TRF yang menerima TRF (200 mg/kg bw); kumpulan FNT yang diadministrasi FNT (20 mg/kg bw) dan kumpulan TRF+FNT yang diberi TRF (200 mg/kg bw) 30 min selepas diadministrasi FNT (20 mg/kg bw). FNT dan TRF telah dilarutkan dalam minyak jagung dan semua suplemen telah diberi secara oral untuk 28 hari. Selepas empat minggu suplementasi, tikus TRF+FNT menunjukkan penurunan aras malonaldehid (MDA) dan aktiviti superoksida dismutase (SOD) tetapi aras glutation terturun (GSH) serta protein jumlah lebih tinggi berbanding tikus FNT (p<0.05). Namun, aras karbonil protein (PC) kumpulan TRF+FNT adalah rendah berbanding kumpulan FNT secara tidak signifikan. Kesimpulannya, kajian ini mencadangkan bahawa TRF minyak sawit dapat mengurangkan tekanan oksidatif dan mengelakkan kerosakan otak tikus aruhan FNT secara berkesan.

Kata kunci: Fenitrotion; kerosakan oksidatif; pecahan kaya tokotrienol minyak sawit

REFERENCES

Aksenova, M.V., Aksenov, M.Y., Mactutus, C.F. & Booze, R.M. 2005. Cell culture models of oxidative stress and injury in the central nervous system. Current Neurovascular Research 2: 73-89.

Amara, B.I., Soudani, N., Hakim, A., Troudi, A., Zeghal, K.M., Boudawara, T. & Zeghal, N. 2011. Selenium and vitamin E, natural antioxidants, protect rat cerebral cortex against dimethoate-induced neurotoxocity. Pesticide Biochemistry and Physiology 101: 165-174.

Beyer, W.F. & Fridovich, I. 1987. Assaying for the superoxide dismutase activity: Some large consequences of minor changes in condition. Analytical Biochemistry 161: 559-566.

Budin, S.B., Yusof, K.M., Idris, M.H.M., Hamid, Z.A. & Mohamed, J. 2011. Tocotrienol-rich fraction of palm oil reduced pancreatic damage and oxidative stress in streptozotocin-induced diabetic rats. Australian Journal of Basic and Applied Sciences 5: 2367-2374.

Budin, S.B., Othman, F., Louis, S.R., Bakar, M.A., Das, S. & Mohamed, J. 2009. The effects of palm oil tocotrienol rich fraction supplementation on biochemical parameters, oxidative stress and the vascular wall of streptozotocin induced diabetic rats. Clinics 64: 235-244.

Contreras, M.A., Greiner, R.S., Chang, M.C., Myers, C.S., Salem, N. Jr. & Rapoport, S.I. 2000. Nutritional deprivation of alpha-linolenic acid decreases but does not abolish turnover and availability of unacylateddocosahexaenoic acid and docosahexaenoyl-CoA in rat brain. Journal of Neurochemistry 75: 2392-2400.

El-Demerdash, F.M. 2011. Lipid peroxidation, oxidative stress and acetylcholinesterase in rat brain exposed to organophosphate and pyrethroid insecticides. Food and Chemical Toxicology 49: 1346-1352.

Ellman, G.L., Courtney, K.D. & Andres, V.J. 1961. A new and rapid colorimeteric determination of acetylcholinesterase activity. Biochemical Pharmacology 7: 88-95.

Ellman, G.L. 1959. Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics 82: 70-77.

Frank, J., Chin, X.W.D., Schrader, C., Eckert, G.P. & Rimbach, G. 2012. Do tocotrienol have potential as neuroprotective dietary factors? Ageing Research Reviews 11: 163-180.

Goel, A., Dani, V. & Dhawan, D.K. 2005. Protective effects of zinc on lipid peroxidation, antioxidant enzymes and hepatic histoarchitecture in chlorpyrifos-induced toxicity. Chemico- Biological Interactions 156: 131-140.

Hunter, M.I.S. & Jamaludin, M. 1986. Plasma antioxidants and lipid peroxidation product in Duchenne muscular dystrophy. Clinica Chimica Acta155: 123-132.

Kamat, J.P. & Devasagayam, T.P. 1995. Tocotrienols from palm oil as potent inhibitors of lipid peroxidation and protein oxidation in rat brain mitochondria. Neuroscience Letter 195: 179-182.

Kwong, T.C. 2002. Organophosphate pesticides: Biochemistry and clinical toxicology. Therapeutic Drug Monitoring 24: 144-149.

Levine, R.L., Garland, D., Oliver, C.N., Amici, A., Climent, I., Lenz, A.G., Ahn, B.W., Shaltiel, S. & Stadtman, E.R. 1990. Determination of carbonyl content in oxidatively modified proteins. Methods in Enzymology 186: 464-478.

Lukaszewicz-Hussain, A. 2008. Subchronic intoxication with chlorfenvinphos, an organophosphate insecticide, affects rat brain antioxidative enzymes and glutathione levels. Food and Chemical Toxicology 46: 82-86.

Merad-Boudia, M., Nicole, A., Santiard-Baron, D., Saille, C. & Ceballos-Picot, I. 1998. Mitochondrial impairment as an early event in the process of apoptosis induced by glutathione depletion in neuronal cells: Relevance to Parkinson’s disease. Biochemical Pharmacology 56: 645-655.

Saulsbury, M.D., Heyliger, S.O., Wang, K. & Johnson, D.J. 2009. Chlorpyrifos induced oxidative stress in oligodendrocyte progenitor cells. Toxicology 259: 1-9.

Shirpoor, A., Salami, S., Khadem-Ansari, M.H., Ilkhanizadeh, B., Pakdel, F.G. & Khademvatani, K. 2009. Cardioprotective effect of vitamin E: rescues of diabetes-induced cardiac malfunction, oxidative stress, and apoptosis in rat. Journal of Diabetes and its Complications 23: 310-316.

Theriault, A., Chao, J.T., Wang, Q., Gapor, A. & Adeli, K. 1999. Tocotrienol: A review of its therapeutic potential. Clinical Biochemistry 32: 309-319.

Tiwari, V., Kuhad, A., Bishnoi, M. & Chopra, K. 2009. Chronic treatment with tocotrienol, an isoform of vitamin E, prevents intracerebroventricular streptozotocin-induced cognitive impairment and oxidative-nitrosative stress in rats. Pharmacological Biochemistry and Behaviour93: 296-303.

Uygun, U., Koksel, H. & Atli, A. 2005. Residue levels of malathion and its metabolites and fenitrothion in post-haverst treated wheat during storage, milling and baking. Food Chemistry 92: 643-647.

Xavier, R., Rekha, K. & Bairy, K.L. 2004. Health perspective of pesticide exposure and dietary management. Malaysian Journal of Nutrition 10: 39-51.

Yoshida, Y., Saito, Y., Jones, L.S. & Shigeri, Y. 2007. Chemical reactivities and physical effects in comparison between tocopherols and tocotrienols: Physiological significance and prospects as antioxidants. Journal of Bioscience and Bioengineering 104: 439-445.

*Corresponding author; email: balkis6466@yahoo.com.my