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Sains Malaysiana 44(12)(2015): 1693–1699
Development of the Compact UV Illuminator with Single UV Lamp (Pembangunan Iluminator UV
Padat dengan Satu Lampu UV) JONG-DAE KIM2,3, DEUK-JU LEE1,3, SANG-YOON KIM1,3, YU-SEOP KIM2,3,
HYE-JEONG SONG2,3 & CHAN-YOUNG PARK*2,3
Deuk-Ju Lee & Sang-Yoon Kim
1Department
of Computer Engineering, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gang-won-do,
200-702, Korea
2Department
of Ubiquitous Computing, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gang-won-do,
200-702, Korea
3Bio-IT
Research Center, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gangwon-do,
200-702,
Korea
Received: 1 September 2014/Accepted: 23
June 2015
ABSTRACT
This paper presents a low-cost method of constructing the compact UV illuminator,
which is considered as an important component of a gel documentation system.
The procedure involves using a smallest-possible UV lamp
and a motor which moves the UV lamp in the UV illuminator
instead of conventional 4 UV lamps. A comparative analysis of
images produced by using the commercial gel documentation system and our
prototype was carried out. These comparisons were done in real DNA gel
as well as a reference plate made of quantum dot. The plate was composed of the
chambers filled with various densities of the quantum dot instead of the
Agarose gel containing the ETBR in order to increase the accuracy
of comparison and the convenience of experiments. Despite the use of only 1 UV lamp,
the proposed system demonstrated a similar imaging performance compared with
the conventional gel documentation system equipped with 4 UV lamps,
resulting in the great reduction of the system cost.
Keywords: DNA detection; gel documentation
system; gel image analysis; quantum dot; UV illuminator
ABSTRAK Kertas ini membentangkan kaedah kos rendah membina iluminator UV
yang padat dan dianggap sebagai satu komponen yang
penting dalam sistem dokumentasi gel. Prosedur ini melibatkan penggunaan
lampu UV terkecil dan motor yang menggerakkan
lampu UV dalam iluminator UV dan
bukannya lampu konvensional 4 UV. Satu analisis perbandingan
imej yang dihasilkan dengan menggunakan sistem dokumentasi perdagangan
gel dan prototip kami telah dijalankan. Perbandingan ini telah dijalankan
dalam gel DNA
sebenar serta plat rujukan buatan daripada dot kuantum.
Plat tersebut terdiri daripada ruang dalam yang dipenuhi dengan
pelbagai kepadatan dot kuantum dan bukan gel Agarosa yang mengandungi
ETBR untuk meningkatkan ketepatan perbandingan
dan kemudahan uji kaji. Walaupun hanya menggunakan 1 lampu UV,
sistem yang dicadangkan menunjukkan prestasi imej yang sama berbanding
dengan sistem dokumentasi gel konvensional dilengkapi dengan 4 lampu
UV,
seterusnya mengurangkan sistem kos.
Kata kunci: Dot kuantum; gel
analisis imej; pengesanan DNA; sistem dokumentasi
gel; UV iluminator
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*Corresponding author; email: cypark@hallym.ac.kr
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