Sains Malaysiana 45(12)(2016): 1931–1938

http://dx.doi.org/10.17576/jsm-2016-4512-18

 

Kesan Psikostres terhadap Kerosakan DNA dan Ketaknormalan Titisan Sitoplasma Sperma Manusia

(Effect of Psychostress on DNA Damage and Cytoplasm Droplet Abnormality in Human Sperm)

 

NORHAMIZAN HASHIM¹, KHAIRUL OSMAN¹*, SITI FATIMAH IBRAHIM², ROSLIAH HARUN³ & RAFEAH PAKRI MOHAMED²

 

¹Program Sains Forensik, Fakulti Sains Kesihatan, Universiti Kebangsaan Malaysia, 50300 Jalan Raja Muda, Kuala Lumpur, Malaysia

 

²Jabatan Fisiologi, Fakulti Perubatan, Universiti Kebangsaan Malaysia, Jalan Yaacob Latiff

56000 Kuala Lumpur, Wilayah Persekutuan, Malaysia

 

³Bahagian Reproduksi Manusia, Lembaga Penduduk dan Pembangunan Keluarga Negara (LPPKN)

Jalan Raja Laut, 50350 Kuala Lumpur, Wilayah Persekutuan, Malaysia

 

Received: 17 February 2016/Accepted: 14 October 2016

 

ABSTRAK

Ketidaksuburan idiopati dalam kalangan lelaki telah dikaitkan dengan kesan psikostres. Walaupun begitu, hubungan langsung antara psikostres dan ketaknormalan kualiti semen masih samar. Maka, kajian ini dijalankan untuk menentukan kesan psikostres terhadap kualiti semen terutama kesan berdasarkan residu sitoplasma dan kerosakan DNA sperma. Dalam kajian ini, responden lelaki berumur antara 25-45 tahun dipilih secara rawak dalam kalangan pesakit yang mendapatkan rawatan di Pusat Kesuburan Lembaga Penduduk dan Pembangunan Keluarga Negara (LPPKN). Seramai 331 responden akhirnya telah dipilih daripada 628 responden selepas mengambil kira faktor penolakan. Setiap responden perlu menjawab borang keizinan dan soal selidik GHQ-12 bagi penentuan tahap stres sebelum pengambilan sampel semen mengikut piawaian WHO (2010). Tahap stres diukur berdasarkan keadaan semasa responden dalam tempoh 3-4 minggu sebelum kajian. Analisis semen, pewarnaan papanicolau dan asai komet neutral digunakan untuk penentuan kualiti semen dan kerosakan DNA sperma. Keputusan menunjukkan tidak terdapat hubungan yang signifikan antara psikostres dan ketaknormalan residu sitoplasma (U=895.50, p=0.08). Namun begitu, psikostres memberi kesan kepada peratus morfologi normal (U=6317.50, p<0.05) dan kerosakan DNA sperma (U=1047.00, p<0.01). Kesimpulannya, psikostres kronik boleh menjejaskan kualiti semen dan kerosakan DNA sperma serta mempengaruhi kesuburan.

 

Kata kunci: Kerosakan DNA sperma; psikostres; titisan sitoplasma sperma; sperma manusia

 

 

ABSTRACT

Idiopathic infertility has been correlated with psychostress in men. Nevertheless, direct relationship of low semen quality to psychostress still remains unclear. Hence, this study was conducted to evaluate the effect of psychostress on semen quality especially on the sperms DNA and cytoplasm droplet abnormality. In this study, respondents within 25-45 years old men were selected randomly among patients of LPPKN. Out of 628, only 331 respondents were selected after reviewing research protocol exclusion criteria. Each respondent was required to fill up a consent form and a GHQ-12 form. The latter was used to measure respondent’s stress levels. Stress level was based on respondent’s condition 3-4 weeks prior to this study. Semen sample was collected based on WHO (2010) standard semen sample collection protocol. Semen analysis, papanicoulau stain and neutral comet assay were used to measure the semen quality, percentage of sperm cytoplasm droplet abnormality and sperm DNA damage, respectively. The results showed that there was no significant association between psychostress and sperm cytoplasm droplet abnormality (U=895.50, p=0.08). Nevertheless, the percentage of normal morphology (U=6317.50, p<0.05) and DNA damage (U=1047.00, p<0.01) were affected by psychostress. As a conclusion, chronic psychostress may affect the semen quality and induced sperm DNA damage and influences the fertility eventually.

 

Keywords: Human sperm; psycostress; sperm cytoplasm droplet; sperm DNA damage

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*Corresponding author; email: khairos@ukm.edu.my