Sains Malaysiana 45(4)(2016): 609–613

Aktiviti Anti-Acanthamoeba oleh Disinfektan Kanta Sentuh Pelbagai Guna terhadap Sista Acanthamoeba Pencilan Klinikal dan Persekitaran

(Anti-Acanthamoeba Activity of Multi-purpose Contact Lens Disinfecting Solutions against Acanthamoeba Cysts of Clinical and Environmental Isolates)

 

MOHAMED KAMEL ABD GHANI¹*, SYAMIMI OMAR¹, ANISAH NORDIN², YUSOF SUBOH², NORAINA AB RAHIM², WAN OMAR ABDULLAH³ & NORAZAH AHMAD⁴

 

¹Program Sains Bioperubatan, Fakulti Sains Kesihatan, Universiti Kebangsaan Malaysia

Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia

 

²Jabatan Parasitologi Perubatan, Fakulti Perubatan, Universiti Kebangsaan Malaysia

Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia

 

³Jabatan Mikrobiologi dan Parasitologi Perubatan, Fakulti Perubatan dan Sains Kesihatan

Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia

 

⁴Unit Bakteriologi, Institut Penyelidikan Perubatan, Jalan Pahang, 50588 Kuala Lumpur

Malaysia

 

Received: 21 August 2014/Accepted: 26 October 2015

 

ABSTRAK

Disinfeksi kanta sentuh yang berkesan adalah sangat penting sebagai langkah pencegahan keratitis Acanthamoeba (AK). Oleh yang demikian, kajian ini dijalankan untuk menilai keberkesanan larutan disinfektan kanta sentuh pelbagai guna terhadap sista Acanthamoeba dengan menggunakan empat pencilan, dua pencilan klinikal; HS 5, HTH 73, dan dua lagi pencilan persekitaran; TTA 1 dan TKA 14. Lima jenis larutan disinfektan pelbagai guna; Complete®, Renu® fresh™, RevitaLens OcuTec®, Opti-Free® Express® dan Solo Care Aqua® telah diuji ke atas pencilan Acanthamoeba tersebut. Masa rendaman adalah berdasarkan masa yang disyorkan oleh pengeluar (4 jam dan 6 jam), 8 jam dan 24 jam. Setiap campuran sista dan larutan disinfektan kanta sentuh dipindahkan ke atas agar tanpa nutrien yang dilapisi Escherichia coli. Plat agar diperiksa di bawah mikroskop songsang setiap hari sehingga hari ke-14 untuk melihat kehadiran trofozoit. Kelima-lima larutan disinfektan pelbagai guna kanta sentuh tidak efektif membunuh kesemua pencilan sista Acanthamoeba yang diuji. Ini menunjukkan bahawa kebanyakan larutan disinfektan pelbagai guna kanta sentuh tidak mempunyai aktiviti anti-Acanthamoeba yang sangat diperlukan bagi mencegah jangkitan keratitis Acanthamoeba dalam kalangan pengguna kanta sentuh.

 

Kata kunci: Acanthamoeba; keberkesanan; larutan disinfektan pelbagai guna kanta sentuh; Malaysia

 

ABSTRACT

Effective disinfection of contact lenses is of paramount importance in the prevention of Acanthamoeba (AK). Therefore, this study was undertaken to evaluate the effectiveness of the multi-purpose contact lens disinfecting solutions against Acanthamoeba cysts of four isolates comprising two clinical isolates; HS 5, HTH 73, and two environmental isolates; TTA 1 and TKA 14. Five multi-purpose contact lens disinfecting solutions; Complete®, Renu® fresh™, RevitaLens OcuTec®, Opti-Free® Express® and Solo Care Aqua® were tested on cyst of the four Acanthamoeba isolates. The soaking times were based on manufactures recommendations (4 and 6 h), 8 h and 24 h. Each mixture of the cysts and disinfecting solution was transferred onto non-nutrient agar seeded with Escherichia coli. The agar plates were examined under inverted microscope daily until day 14 to detect the presence of Acanthamoeba trophozoites. All 5 multi-purpose contact lens disinfecting solutions tested were ineffective at killing all Acanthamoeba cyst isolates tested. This indicates that most multi-purpose contact lens disinfecting solutions do not have anti-Acanthamoeba activity necessary to prevent keratitis amongst the contact lens wearers.

 

Keywords: Acanthamoeba; effectiveness; Malaysia; multi-purpose contact lens disinfecting solution

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*Corresponding author; email: mohamedkamela@yahoo.com