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Sains Malaysiana 46(10)(2017): 1687–1691 http://dx.doi.org/10.17576/jsm-2017-4610-03
Nitric
Oxide Accelerates Mycorrhizal Effects on Plant Growth and Root
Development of Trifoliate Orange (Nitrik Oksida Mempercepatkan
Kesan Mikoriza
ke atas Pertumbuhan
Pokok dan
Perkembangan Akar Oren Trifoliat) LI TIAN1,2, NASRULLAH3,4, XIAO-YUN
HUANG1
& QIANG-SHENG WU1,2,5* 1College of Horticulture
and Gardening, Yangtze University, Jingzhou,
Hubei 434025 China 2Institute of Root
Biology, Yangtze University, Jingzhou,
Hubei 434025, China 3Department of Plant
Sciences, Quaid-I-Azam University, Islamabad, Pakistan 4Department of Biology
and Ecology, Nankai University, Tianjin,
China 5Department of Chemistry,
Faculty of Science, University of Hradec Kralove, Hradec Kralove,
Czech Republic Received: 24 January
2017Accepted: 31 March 2017 ABSTRACT Arbuscular mycorrhizal fungi
(AMF)
actively colonize plant roots and thus enhance plant growth through
different mechanisms. In the present study, trifoliate orange
(Poncirus trifoliata)
seedlings inoculated with Diversispora
versiformis were subjected to 0 and 0.2 mmol/L sodium nitroprusside (SNP,
a nitric oxide donor) treatments. After eight weeks, exogenous
SNP considerably
increased root mycorrhizal colonization by 25%, showing a positive
stimulating effect of NO on mycorrhizal formation. Mycorrhizal inoculation significantly
increased plant growth performance (height, stem diameter, leaf
number and shoot and root dry weight) and root traits (length,
projected area, surface area, volume and number of 2nd and 3rd
order lateral roots) than non-mycorrhizal treatment and NO (exogenous SNP treatment)
heavily strengthened the mycorrhizal effects. Moreover, NO and
mycorrhization induced more fine root
(0-0.5 cm) formation. There was an opposite changed trend in root
sucrose and leaf and root glucose contents by SNP in AMF versus
non-AMF seedlings. All these results implied that NO
plays important roles in mycorrhizal formation and
development and also accelerates mycorrhizal effects on plant
growth and root development of trifoliate orange. Keywords: Arbuscular mycorrhizal
fungi; carbohydrate; citrus; nitric oxide; sodium nitroprusside
ABSTRAK Kulat mikoriza arbuskula
(AMF)
mengkoloni akar
tumbuhan secara aktif dan seterusnya
menggalakkan pertumbuhan
pokok melalui mekanisme
berbeza. Dalam
kajian ini,
benih oren
trifoliat (Poncirus trifoliata) yang diinokulasi
dengan Diversispora versiformis telah diberikan rawatan 0 dan 0.2 mmol/L sodium nitropussida (SNP, penderma
nitrik oksida).
Selepas lapan minggu, SNP eksogenus didapati meningkatkan pengkolonian akar mikoriza sebanyak
25% dan ini
menunjukkan kesan rangsangan positif NO
terhadap pembentukan mikoriza. Penginokulasian mikoriza meningkatkan
prestasi pertumbuhan
pokok secara signifikan
(tinggi, diameter batang,
jumlah daun dan
berat akar
kering) dan ciri
akar (panjang,
luas unjuran, luas
permukaan, isi
padu, bilangan akar lateral peringkat ke-2 dan ke-3) berbanding rawatan tanpa mikoriza
serta NO (rawatan
SNP
eksogenus) mengukuhkan
lagi kesan
mikoriza. Di samping
itu, rawatan
NO
dan mikoriza mengaruh
lebih banyak
pembentukan akar halus (0-0.5 cm). Terdapat trend perubahan
bertentangan pada
kandungan sukrosa akar, daun serta
glukosa akar
oleh SNP dalam benih AMF berbanding
tanpa AMF. Keseluruhan
keputusan kajian
ini menunjukkan bahawa NO memainkan
peranan penting
dalam pembentukan dan perkembangan mikoriza, malah mempercepatkan kesan mikoriza ke atas
pertumbuhan pokok
dan perkembangan akar oren
trifoliat. Kata kunci: Cendawan
mikoriza asbukula;
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