Sains Malaysiana 46(12)(2017): 2273–2280

http://dx.doi.org/10.17576/jsm-2017-4612-03

Ovarian Maturation Stages of Wild and Captive Mud Crab, Scylla olivacea Fed with Two Diets

(Peringkat Kematangan Ovari bagi Ketam Lumpur Liar dan Kurungan Scylla olivacea Diberi Makan dengan Dua Diet)

AZMIE GHAZALI¹, NOORDIYANA MAT NOORDIN², AMBOK BOLONG ABOL-MUNAFI¹,

MOHAMAD N. AZRA¹ & MHD IKHWANUDDIN¹

¹Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu Darul Iman, Malaysia

²School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu,

21030 Kuala Terengganu, Terengganu Darul Iman, Malaysia

Received: 25 July 2016/Accepted: 25 May 2017

ABSTRACT

This study was aimed to determine the ovarian maturation stages of wild and captive orange mud crab, Scylla olivacea fed with different diets via gonadosomatic status, oocyte diameter and histological examinations. Captive crabs were fed with blood cockle, Anadara granosa, or fish, Decapterus spp. Through the histological examinations, ovarian maturation stages of wild and captive S. olivaceawas classified into four stages: Immature (Stage 1), Early maturing (Stage 2), Pre-maturing (Stage 3) and Fully matured (Stage 4). Gonadosomatic Index of wild and captive crabs remained low during immature and 2 but increased significantly (p<0.05) in pre-maturing and 4 ovaries. Oocytes size were significantly different (p<0.05) in all ovarian maturation stages of wild and captive crabs. Follicle cells surround the oocyte of immature ovary and small yolk globules start to appear in early maturing ovary with large nucleus size. Oocyte size increased significantly (p<0.05) and yolk globule obviously appeared in pre-maturing ovary. Large and fused yolk globules appeared in the oocytes of fully matured ovary with nucleus was barely visible. The present study revealed that, ovarian maturation stages of S. olivacea are closely related to its morphological appearance and cellular development.

Keywords: Aquatic science; fisheries; invertebrate; nutrition; reproductive biology

ABSTRAK

Kajian ini bertujuan untuk menentukan tahap pematangan ovari bagi ketah lumpur liar dan kurangan jenisScylla olivacea yang diberi makan dua diet yang berlainan dengan melalui status gonadosomatik, diameter oosit dan pemeriksaan histologi. Ketah lumpur telah diberi makanan seperti kerang darah seperti Anadara granosa, atau ikan seperti Decapterus spp. Peringkat pematangan ovari bagiS. olivacea liar dan kurungan telah dibahagi kepada empat peringkat melalui pemeriksaan histologi: Tidak matang (Tahap 1), Matang awal (Tahap 2), Pra-matang (Tahap 3) dan Matang sepenuh (Tahap 4). Indeks Gonadosomatik ketam lumpur liar dan kurungan adalah rendah pada tahap tidak matang dan 2 ovari tetapi meningkat dengan ketara (p<0.05) bagi pra-matang dan 4 ovari. Saiz oosit adalah berbeza dengan ketara (p<0.05) bagi semua peringkat pematangan ovari bagi ketam lumpur liar dan kurangan. Sel-sel folikel mengelilingi oosit ovuri yang tidak matang dan kuning telur yang kecil mula berlaku pada tahap matang awal ovari dengan saiz nukleus besar. Saiz oosit meningkat secara ketara (p<0.05) dan globul telur kuning jelas kelihatan pada tahap pra-matang ovari.

Kata kunci: Biologi pembakan; invertebrat; nutrisi; perikanan; sains akuatik

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*Corresponding author; email: ikhwanuddin@umt.edu.my