Sains Malaysiana 46(12)(2017): 2417–2423

http://dx.doi.org/10.17576/jsm-2017-4612-17

 

Kinetin Induces Chromosomal Abnormalities in African Blue Lily (Agapanthus praecox ssp. minimus) Grown in In Vitro

(Aruhan Kinetin Keabnormalan Kromosom Telipuk Biru Afrika (Agapanthus praecox ssp. minimus) yang Ditanam secara In Vitro

 

JAMILAH SYAFAWATI YAACOB^1,2* & ROSNA MAT TAHA²

 

¹Centre for Research in Biotechnology for Agriculture (CEBAR), Faculty of Science

University of Malaya, 50603 Kuala Lumpur, Federal Territory, Malaysia

 

²Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Federal Territory, Malaysia

 

Received: 28 January 2015/Accepted: 14 April 2017

 

ABSTRACT

Kinetin has been reported to exert inhibitory effect when used in tissue culture and in some cases reverse the action of auxin and cause growth inhibition and retardation of root formations. Kinetin also acts as ‘mitotic poison’, mimicking the effect of pesticides and toxic chemicals and interferes in mitosis mechanism of plants. The effect of kinetin on size of cell and nucleus as well as chromosome behaviour in root tip meristems of Agapanthus praecox ssp. minimus was studied. The results showed that prolong exposure to kinetin caused chromosome abnormalities to occur more frequently. Chromosome breakage yielded fragmented chromosomes, while abnormal spindle fibers caused delay in chromosome movement, termed as laggard chromosomes. Abnormal nucleus was also observed with kinetin treatments, such as micronucleus, binucleated and tripolar cells.

 

Keywords: Binucleated; chromosome behavior; cytomixis; fragmented chromosome; kinetin; laggard

 

ABSTRAK

Kinetin telah dilaporkan memberi kesan merencat apabila digunakan dalam kultur tisu dan dalam beberapa kes membalikkan tindakan auksin dan menyebabkan pertumbuhan dan pembentukan akar terencat. Kinetin juga bertindak sebagai ‘racun mitosis’, meniru kesan racun perosak dan bahan kimia toksik dan mengganggu mekanisma mitosis dalam tumbuhan. Kesan kinetin pada saiz sel dan nukleus dan juga tingkah laku kromosom dalam hujung meristem akar Agapanthus praecox ssp. minimus telah dikaji. Keputusan kajian menunjukkan bahawa pendedahan berpanjangan kepada kinetin telah menyebabkan keabnormalan kromosom berlaku lebih kerap. Pecahan kromosom menghasilkan kromosom terserpih, manakala gentian gelendong yang tidak normal menyebabkan kelewatan dalam pergerakan kromosom, yang digelar sebagai kromosom yang terkemudian. Keabnormalan nukleus juga diperhatikan dengan rawatan kinetin, seperti nukleus mikro, binukleus dan sel tripolar.

 

Kata kunci: Binukleat; kelakuan kromosom; kinetin; kromosom terserpih; sitomiksis; terkemudian

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*Corresponding author; email: jamilahsyafawati@um.edu.my