Sains Malaysiana 46(1)(2017): 51–58

http://dx.doi.org/10.17576/jsm-2017-4601-07

Biohydrogen Productions from Hydrolysate of Water Hyacinth Stem (Eichhornia crassipes) Using Anaerobic Mixed Cultures

(Pengeluaran Biohidrogen daripada Hidrolisat Batang Keladi Bunting (Eichhornia crassipes)

Menggunakan Kultur Campuran Anaerob)

SAKCHAI PATTRA¹* & SUREEWAN SITTIJUNDA²

¹Community Public Health Program, Faculty of Arts and Science, Chaiyaphum Rajabhat University, Chaiyaphum 36000, Thailand

²Faculty of Environment and Resource Studies, Mahidol University, Nakhon Pathom 73170

Thailand

Received: 22 April 2015/Accepted: 14 April 2016

ABSTRACT

Response surface methodology (RSM) with central composite design (CCD) was applied to optimize key factors affecting hydrogen production (HP) from diluted acid hydrolysate of water-hyacinth stem (WHS) by heat-treated anaerobic sludge in a batch fermentation process. Key factors affecting namely substrate concentration and initial pH was investigated. The results indicated that substrate concentration and initial pH had significantly effects on HP (p<0.05). A maximum HP hydrogen production rate and hydrogen yield of 182.7 mmol H2/L, 2.81 mmol H2/L h and 0.84 mol H2/mol hexose were obtained under the optimum conditions i.e. substrate concentration of 4.06 g/L and initial pH of 5.81. The total energy production from the fermentative of WHS hydrolysate was 1.97 kJ.

Keywords: Central composite design (CCD); dilute acid hydrolysis; hydrogen production; response surface methodology (RSM); water-hyacinth

ABSTRAK

Kaedah permukaan tindak balas (RSM) dan pusat reka bentuk tergubah (CCD) digunakan untuk mengoptimumkan faktor utama yang mempengaruhi pengeluaran hidrogen (HP) daripada hidrolisat asid cair batang keladi bunting (WHS) melalui enapcemar anaerobik terawat-haba di dalam proses penapaian kumpulan. Faktor utama yang mempengaruhi kepekatan substrat dan pH awal dikaji. Keputusan menunjukkan bahawa kepekatan substrat dan pH awal telah memberi kesan secara signifikan kepada HP (p<0.05). Kadar pengeluaran hidrogen HP maksimum dan hasil hidrogen ialah 182.7 mmol H2/L, 2.81 mmol H2/L h dan 0.84 mol H2/mol heksosa telah diperoleh pada keadaan optimum iaitu kepekatan substrat 4.06 g/L dan pH awal 5.81. Jumlah pengeluaran tenaga daripada penapaian WHS hidrolisat adalah 1.97 kJ.

Kata kunci: Hidrolisis asid cair; kaedah permukaan tindak balas (RSM); keladi bunting; pengeluaran hidrogen; pusat reka bentuk tergubah (CCD)

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*Corresponding author; email: sakpattra@gmail.com