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Sains Malaysiana 46(4)(2017):
521–528 http://dx.doi.org/10.17576/jsm-2017-4604-02
Analysis of Simple Sequence
Repeat Markers Linked to Submergence Tolerance on Newly Developed
Rice Lines Derived from MR263 × Swarna-Sub1 (Analisis
Penanda Ulangan Jujukan Ringkas terhadap Titisan Padi Baharu Dibangunkan yang Toleransi
Tenggelam daripada Kacukan
MR263 × Swarna -Sub1) WELLAND
COSMAS
MOJULAT1,
MOHD
RAFII YUSOP1,2*,
MOHD
RAZI
ISMAIL1,2,
ABDUL
SHUKOR
JURAIMI1,
ABDUL
RAHIM HARUN3,
FAHIM
AHMED1,
FATAH
ABRO
TANWEER1,2
& MD. ABDUL LATIF5 1Department of Crop Science,
Faculty of Agriculture, Universiti Putra
Malaysia, 43400 UPM Serdang, Selangor
Darul Ehsan, Malaysia 2Institute of Tropical
Agriculture and Food Security, Universiti
Putra Malaysia, 43400 UPM Serdang, Selangor
Darul Ehsan, Malaysia 3Agrotechnology and Bioscience
Division, Malaysian Nuclear Agency, Bangi 43000 Kajang, Selangor Darul Ehsan, Malaysia 4Department of Plant Breeding
and Genetics, Faculty of Crop Production, Sindh Agriculture University
Tandojam, Sindh, Pakistan 5Bangladesh Rice Research
Institute (BRRI), Gazipur, Dhaka, Bangladesh Received: 12 January
2016/Accepted: 24 September 2016 ABSTRACT Nowadays, in extreme
changing environments, development of submergence tolerance variety
is necessary for ensuring crop production stability where, it is
known that Malaysian commercial rice varieties such as MR219,
MR220
and MR263 were severely susceptible to submergence. First step
towards the development of submergence tolerance variety starts
with the breeding program by crossing MR263 and Swarna-Sub1. Marker-assisted selection (MAS)
was carried out through the utilization of simple sequence repeats
(SSR)
markers, considering its reliability as pre-selection tools to conduct
this research. F1 generations plants were confirmed by tightly linked markers.
In case of background study, out of 180 SSR markers, 38 were found
polymorphic between two parents. Association of molecular markers
and submergence tolerance were determined using Chi-square test.
MR263
× Swarna-Sub1 F2 lines were tested for Sub1 gene
conformation using the markers RM8300 and RM219.
These markers showed a good fit to the expected marker segregation
ratio (1:2:1) in a Mendelian single gene model (DF=1.0,
p≤0.05). Eleven homozygous lines with Sub1 gene
out of 256 were selected for future development of submergence tolerant
varieties. Eleven lines were selected based on phenotypic study
and agronomic performance. Keywords: Marker-assisted
selection; rice; Sub1; submergence
tolerance; SSR ABSTRAK Pembangunan
varieti padi
yang toleransi tenggelam adalah penting bagi memastikan kestabilan pengeluaran tanaman akibat perubahan melampau alam sekitar yang tidak menentu. Varieti padi komersial Malaysia seperti MR219, MR220
dan MR263 telah
dikenal pasti
terjejas teruk akibat tenggelam. Langkah pertama ke arah
pembangunan varieti
yang toleransi tenggelam bermula dengan program pembiakbakaan MR263 dan
Swarna-Sub1. Dalam
kajian ini, pemilihan
berbantukan penanda
(MAS)
telah dijalankan
dengan menggunakan penanda ulangan jujukan ringkas (SSR)
yang disahkan kebolehpercayannya
sebagai alat
pra-pemilih. Pokok generasi
F1
telah disahkan
oleh penanda
yang terkait erat. Dalam kajian latar belakang,
daripada 180 penanda
SSR,
38 penanda adalah
polimorfik antara kedua-dua induk. Hubungan antara penanda ulangan
jujukan ringkas dan
toleransi tenggelam ditentukan dengan menggunakan ujian Chi-square.
Titisan F2 MR263
× Swarna-Sub1 telah diuji
untuk korformasi
gen Sub1 dengan menggunakan penanda RM8300 dan
RM219.
Penanda ini
menunjukkan keserasian yang baik dengan kadar segregasi yang dijangka (1:2:1) dalam model gen
tunggal Mendel (DF=1.0, p≤0.05).
Sebelas titisan homozigot yang mempunyai gen Sub1
telah dipilih
daripada 256 titisan untuk pembangunan masa depan varieti
padi yang toleransi tenggelam.
Sebelas titisan ini dipilih berdasarkan kajian fenotip dan prestasi agronomi. Kata kunci: Padi;
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