Sains Malaysiana 46(9)(2017):
1471–1477
http://dx.doi.org/10.17576/jsm-2017-4609-16
Gynura procumbens Leaf
Improves Blood Glucose Level, Restores Fertility and Libido of Diabetic-Induced
Male Rats
(Daun Gynura procumbens Meningkatkan Aras Glukosa Darah
serta Memulihkan Kesuburan dan Libido Tikus Jantan Aruhan Diabetik)
KHAIDATUL AKMAR
KAMARUZAMAN
& MAHANEM MAT NOOR*
School of Biosciences and Biotechnology, Faculty
of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi,
Selangor Darul Ehsan, Malaysia
Received: 31 August 2016/Accepted: 1
April 2017
ABSTRACT
Gynura
procumbens (GP) has been widely used in
traditional medicine due to its ability in treating various types of diseases.
This study was carried out to determine the effects of GP on
blood glucose level, fertility and libido of streptozotocin-induced male rats.
A total of 42 male rats were randomly sorted into six groups; normal, negative
and positive controls and three treated groups with different dosages of GP aqueous
extract (GPAE); 150, 300 and 450 mg/kg. Each group was treated via
oral feeding for 14 consecutive days. The rats were sacrificed on day 15 for
further analysis. Administration of GPAE significantly lowered the
fasting blood glucose (FBG) level and increased the plasma
testosterone level of diabetic-induced rats compared to the positive and
negative control groups. There was also a significant increase in the sperm
quality as well as the fertility of GPAE treated groups compared to
the negative and positive control groups. Sexual behaviour analysis
demonstrated that GPAE is able to facilitate the
expression of sexual behaviour significantly; increased mounting frequency and
reduced mounting latency. These lines of evidence suggested that GPAE can act as antihyperglycaemic, profertility and libido agents.
However, its mechanism(s) remains unresolved.
Keywords:
Diabetic; fertility; Gynura procumbens; sexual behaviour; testosterone
ABSTRAK
Gynura procumbens
(GP) telah digunakan
secara meluas
dalam perubatan tradisional kerana keupayaannya dalam merawat pelbagai jenis penyakit. Kajian ini telah dijalankan
untuk menentukan
kesan GP ke atas aras
glukosa darah,
kesuburan dan libido tikus jantan yang diaruh streptozotocin. Sejumlah 42 ekor tikus jantan
telah diasingkan
secara rawak kepada
enam kumpulan;
kawalan normal, negatif dan positif serta
tiga kumpulan
yang dirawat dengan dos berbeza ekstrak akues GP (GPAE);
150, 300 dan 450 mg/kg. Setiap kumpulan
diberi perlakuan
secara oral selama 14 hari berturut-turut. Tikus dikorbankan
pada hari ke-15 untuk
analisis lanjut. Perlakuan GPAE dengan
signifikan telah
menurunkan tahap glukosa darah berpuasa
(FBG)
dan meningkatkan
aras testosteron
plasma tikus diaruh
diabetes berbanding dengan
kumpulan kawalan positif dan negatif.
Terdapat
juga peningkatan signifikan
dalam kualiti
sperma serta kesuburan
kumpulan rawatan
GPAE
berbanding dengan
kumpulan kawalan
negatif dan positif.
Kajian
kelakuan seksual menunjukkan bahawa GPAE
ini dapat
meningkatkan kelakuan seksual dengan signifikan; meningkatkan kekerapan pemanjatan dan mengurangkan tempoh pendam pemanjatan.
Bukti
ini mencadangkan bahawa GPAE boleh
bertindak sebagai agen anti hiperglisemik, prokesuburan dan libido.
Walau bagaimanapun mekanisme ini masih belum
diketahui.
Kata kunci: Diabetis melitus; Gynura procumbens; kelakuan seksual;
kesuburan; testosteron
REFERENCES
Adaikan,
P.G. 2011. Abstract 17. Decline in testosterone: Relationship to libido and
sexual dysfunction. Journal of Men’s Health 8(Suppl. 1): S103.
Agmo,
A. 1997. Protocol male rat sexual behaviour. Brain Research Protocols 1:
203-209.
Akowuah,
G.A., Sadikun, A. & Mariam, A. 2002. Flavonoid identification and
hypo-glycemic studies of butanol fraction from Gynura procumbens. Pharmaceutical
Biology 40: 405-410.
Akbarzadeh,
A., Norouzian, D., Mehrabi, M.R., Jamshidi, Sh., Farhangi, A., Allah Verdi, A.,
Mofidian, S.M.A. & Lame Rad, B. 2007. Induction of diabetes by
Streptozotocin in rats. Indian Journal of Clinical Biochemistry 22(2):
60-64.
Algariri,
K., Meng, K.Y., Atangwho, I.J., Asmawi, M.Z., Sadikun, A., Murugiyah, V. &
Ismail, N. 2013. Hypoglycemic and anti-hyperglycemic study of Gynura
procumbens leaf extracts. Asian Pacific Journal of Tropical Biomedicine 3(5):
358-366.
Alvarez,
J.G. & Storey, B.T. 1995. Differential incorporation of fatty acids into
and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Molecular
Reproduction and Development 42: 334-346.
Alvez,
M.G., Martins, A.D., Vaz, C.V., Correia, S., Moreira, P.I., Oliveira, P.F.
& Socorro, S. 2014. Metformin and male reproduction: Effects on Sertoli
cells metabolism. British Journal of Pharmacology 171: 1032-1042
Alvez,
M.G., Martins, A.D., Caraco, J.E., Socorro, S. & Oliveira, P.F. 2013a.
Molecular mechanisms beyond glucose transport in diabetes-related male
infertility. Biochimica et Biophysica Acta 1832: 626-635.
Alvez,
M.G., Martins, A.D., Caraco, J.E., Socorro, S. & Oliveira, P.F. 2013b.
Diabetes, insulin mediated glucose metabolism and Sertoli/blood-testes barrier
function. Tissue Barriers 1: 1-12.
Alvez,
M.G., Rato, L., Carvaiho, R.A., Moreira, P.I., Socorro, S. & Oliveira, P.F.
2013c. Hormonal control of Sertoli cells metabolism regulates spermatogenesis. Cellular
and Molecular Life Sciences 70: 777-793.
Biggers,
J.D., Whitten, W.K. & Whittingham, D.G. 1971. The culture
of mouse embryos in vitro. In Methods in Mammalian Embryology,
edited by Daniel Jr., J.C. San Francisco: W.H. Freeman. pp.
86-116.
Butchi Akondi, R., Kumar, P., Annapurna,
A. & Pujari, M. 2011. Protective effect of rutin and naringin
on sperm quality in streptozotocin (STZ) induced type I diabetic
rats. Iranian Journal of Pharmaceutical Research 10(3):
585-596.
Chauhan,
A. & Agarwal, M. 2009. Assessment of the contraceptive efficacy of the
aqueous extract of Aegel marmelos Corr. leaves in the male albino rats. Human
Fertility 12: 107-118.
Gaochao,
Z., Myers, R., Ying, L., Chen, Y., Xiaolan, S., Judy, F.M., Margaret, W., John,
V., Thomas, D., Nobuharu, F., Nicalso, M., Michael, F.H., Laurie, J.G. &
David, E.M. 2001. Role of AMP-activated protein kinase in mechanism of
metformin action. The Journal of Clinical Investigation 108: 1167-1174.
Gupta,
A.K., Bindal, M.C., Gupta, S.K., Prakash, D.B. & Vedpal. 2013. Aphrodisiac
activity of Semecarpus anacordium nut. International Research Journal
of Pharmacy 4(4): 202-204.
Gupta,
R.S., Kachhawa, J.B.S., Khushalani, V., Tanuar, K. & Joshi, Y.C. 2006.
Effect of Cressa cretica methanol extract on testicular function of
albino rats. Pharmaceuticals Biology 44: 382-388.
Khaki,
A., Nouri, M., Fathiazad, F., Ahmadi-Ashtiani, H.R., Rastgar, H. &
Rezazadeh, S. 2009. Protective effects of quercetin on spermatogenesis in
streptozotocin-induced diabetic rats. Journal of Medicinal Plants 5(8):
57-64.
Ladachart,
T., Prakong, T., Noppadon, K. & Suchinda, M. 2008. Stimulating effects of
quercetin on sperm quality and reproductive organs in adult male rats. Asian
Journal of Andrology 10: 249-258.
Mahanem
Mat Noor & Nani Rahayu. 2012. Anti-hyperglycemic effect of Gynura
procumbens methanolic extract on fertility and libido of induced diabetic
male rats. Sains Malaysiana 41(12): 1549-1556.
Mita, M. &
Hall, F. 1982. Metabolism of round spermatids from rats: Lactate as the
preferred substrate. Biology of Reproduction 26(3): 445-455.
Oonk, R.B. & Grootegoed, J.A. 1987.
Identification of insulin receptors on rat Sertoli cells. Molecular and
Cellular Endocrinology 1(49): 51-62.
Perry, L.M. 1980. Medicinal Plants of
East and Southeast Asia: Attributed Properties and Uses. Cambridge: The MIT
Press. p. 94.
Pusparanee, H., Halimah A.S. &
Mahanem, M.N. 2008. Effects of Gynura procumbens extract and
glibenclamide on sperm quality and specific activity of testicular lactate
dehydrogenase in streptozotocin-induced diabetic rats. Malaysian Journal of
Biochemistry and Molecular Biology 16(2): 10-14.
Rosidah, Mun, F.Y., Amirin, S., Mariam,
A., Gabriel, A.A. & Mohd Zaini Asmawi. 2009. Toxicology evaluation
of standardized methanol extract of Gynura procumbens.
Journal of Ethnopharmacology 123: 244-249.
Shrilata, B. & Muralidhara. 2007.
Early oxidative stress in testes and epidydimal sperm in streptozotocin-induced
diabetic mice: Its progression and genotoxic consequences. J. Reproductive
Toxicology 23(4): 578-587.
Tartarin, P., Moison, D., Guilbert, E.,
Dupont, J., Hebert, R., Rouiller-Fabre, V., Frydman, N., Pozzi, S., Frydman,
R., Lecureuil, C. & Froment, P. 2012. Metformin exposure effects human and
mouse fetal testicular cells. Human Reproduction 27(11): 3304-3314.
Turk, G., Sonmez, M., Aydin, M., Yuce, A.
& Aksoy, H. 2008. Effects of promegranate juice consumption on sperm
quality, spermatogenic cell density, antioxidant activity and testosterone
level in male rats. Clinical Nutrition 27: 289-296.
William, G. & Pickup, J. 2004. Handbook
of Diabetes. Massachusettes: Blakewell Publishing.
World Health Organisation. 2010. Laboratory Manual for the Examination
and Processing of Human Semen. Geneva: WHO Press.
Zurina Hassan, Mun Fei Yam, Mariam Ahmad
& Ahmad Pauzi M. Yusof. 2010. Antidiabteic properties and mechanisms of
action of Gynura procumbens water extract in streptozotocin-induced
diabetic rats. Molecules 15: 9008-9023.
*Corresponding author; email: mahanem@ukm.edu.my