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Sains Malaysiana 46(9)(2017): 1565–1571
http://dx.doi.org/10.17576/jsm-2017-4609-27
Antioxidant and Antimicrobial Activity of the Extracts from
Different Parts of Etlingera sayapensis (Zingiberaceae)
(Aktiviti Antioksida dan Antimikrob bagi Ekstrak daripada
Bahagian Berbeza Etlingera sayapensis (Zingiberaceae))
BEHNAM MAHDAVI1, W.A. YAACOB2* & LAILY B. DIN2
1Department of
Chemistry, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran
2School of Chemical
Sciences and Food Technology, Faculty of Science and Technology
Universiti Kebangsaan
Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia
Received: 9 April
2015/Accepted: 5 March 2017
ABSTRACT
The rhizomes, stems
and leaves of Etlingera sayapensis were extracted
with ethyl acetate, acetone, ethanol and methanol to give 12 extracts.
The total phenolic content (TPC) of the extracts was measured in
this study, together with their antioxidant activity. Assays used
were radical scavenging activity (RSA), β-carotene bleaching (BCB),
ferrous ion chelating (FIC) and ferric reducing antioxidant
power (FRAP). Among the assays, the leaf methanolic extract showed
the highest activity with 35.67 mg GAE/g
(mg of gallic acid per gram of extract) for TPC,
IC50 of 53.43 μg/mL for RSA,
83.24% for BCB, IC50 of 242.43 μg/mL for FIC
(second highest) and 19.53 mg TrE/g (mg of Trolox
per gram of extract) for FRAP.
The measured BCB values for the four leaf extracts were higher than those
of the positive controls of butylated hydroxytoluene (BHT),
gallic acid (GA) and ascorbic acid (AscA) and slightly lower than that
of α-tocopherol (Toc). The antimicrobial activity
of the extracts was also measured using disc-diffusion (DD) and minimum inhibition concentration
(MIC) assays. Among the extracts, only
ethanolic and methanolic extracts showed specific bacterial inhibition
against Bacillus subtilis. Only stem methanolic extract
exhibited specific fungal inhibition against Candida parapsilosis.
The highest activity was shown by this with 9.9 mm and 1.04 mg/mL
for DD and MIC assays
respectively.
Keywords: Antimicrobial activity; antioxidant activity; Etlingera;
Zingiberaceae
ABSTRAK
Rizom, batang dan daun
Etlingera sayapensis telah diekstrak dengan etil asetat, aseton,
etanol dan metanol untuk memberikan 12 ekstrak. Kandungan fenolik
jumlah (TPC) ekstrak telah diukur dalam kajian ini, bersama-sama
dengan aktiviti antioksida mereka. Asai yang digunakan adalah
aktiviti penskavenjing radikal (RSA), pelunturan β-karotena (BCB),
pengkelatan ion ferus (FIC) dan kuasa antioksida penurunan ferik
(FRAP). Dalam kalangan asai ini, ekstrak metanol daun menunjukkan
aktiviti tertinggi dengan 35.67 mg GAE/g
(mg asid galik per gram ekstrak) bagi TPC, IC50 53.43
μg/mL bagi RSA, 83.24% bagi BCB,
IC50 242.43 μg/mL bagi FIC (kedua
tertinggi) dan 19.53 mg TrE/g (mg Troloks per gram ekstrak) bagi
FRAP.
Nilai BCB yang diukur bagi empat ekstrak daun adalah lebih tinggi
daripada nilai kawalan positif hidroksitoluena terbutil (BHT),
asid galik (GA), asid askorbik (AscA) dan rendah sedikit daripada nilai
bagi α-tokoferol (Toc). Aktiviti antimikrob ekstrak juga
telah diukur menggunakan asai peresapan-cakera (DD)
dan kepekatan perencatan minimum (MIC). Dalam kalangan ekstrak,
hanya ekstrak etanol dan metanol menunjukkan perencatan bakteria
yang spesifik melawan Bacillus subtilis. Hanya ekstrak
metanol batang memperlihatkan perencatan fungus yang spesifik
menentang Candida parapsilosis. Aktiviti tertinggi telah
ditunjukkan olehnya dengan 9.9 mm dan 1.04 mg/mL bagi asai DD
dan MIC.
Kata kunci: Aktiviti antimikrob; aktiviti antioksida; Etlingera;
Zingiberaceae
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*Corresponding author; email: wanyaa@ukm.my |
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