Sains Malaysiana 46(9)(2017): 1603–1609

http://dx.doi.org/10.17576/jsm-2017-4609-32

 

The Effect of Tocotrienol-Rich Fraction on Oxidative Liver Damage Induced by Fenitrothion

(Kesan Fraksi Kaya Tokotrienol ke atas Kerosakan Oksidatif Hepar Diaruh Fenitrotion)

 

PUTRI AYU JAYUSMAN, SITI BALKIS BUDIN*, IZATUS SHIMA TAIB

& AHMAD ROHI GHAZALI

 

Programme of Biomedical Science, School of Diagnostic and Applied Health Sciences

Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz

50300 Kuala Lumpur, Federal Territory, Malaysia

 

Received: 24 March 2016/ Accepted: 6 July 2017

 

ABSTRACT

Exposure to organophosphate pesticide including fenitrothion (FNT) has led to many adverse effects on human health. However, a potent antioxidant like palm oil tocotrienol-rich fraction (TRF) can reduce oxidative damage in various pathological conditions, could also reduce the adverse effects by FNT. The aim of this study was to evaluate the effect of TRF on oxidative liver damage in FNT induce hepatotoxicity in experimental rats. A total of 40 male Sprague-Dawley rats were randomly divided into four groups of 10, namely control, TRF, FNT and TRF+FNT group. TRF (200 mg/kg body weight) and FNT (20 m/kg body weight) were administered through oral gavage for 28 days. Corn oil which served as vehicle was given orally to the control group. At the end of the study period, liver and blood was taken for oxidative damage and biochemical evaluation and histological observation. TRF supplementation prevents oxidative liver damage by reducing the hepatic malondialdehyde (MDA) and protein carbonyl (PCO) level significantly. Besides, TRF also restored the endogenous antioxidants particularly reduced glutathione (GSH), glutathione peroxidase (GPx) and ferric reducing/antioxidant power (FRAP). TRF also prevent liver damage by reducing the liver enzymes, alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The attenuation of liver damage by TRF was also showed histologically. In conclusion, TRF supplementation showed a potential in preventing oxidative liver damage in FNT-treated rats by reducing the oxidative damage and improving the antioxidant status.

 

Keywords: Antioxidant; oxidative stress; palm oil; vitamin E

 

ABSTRAK

Pendedahan kepada organofosfat termasuk fenitrotion (FNT) telah menyebabkan kesan sampingan pada kesihatan manusia. Walau bagaimanapun, antioksidan yang poten seperti fraksi kaya tokotrienol minyak sawit (TRF) berupaya untuk mengurangkan kerosakan oksidatif yang mungkin juga berupaya untuk mengurangkan kesan kerosakan FNT. Kajian ini dilakukan untuk menilai kesan TRF ke atas hepatotoksiti tikus aruhan FNT. Sebanyak 40 ekor tikus jantan Sprague-dawley dibahagikan secara rawak kepada empat kumpulan, sepuluh ekor setiap satu iaitu kumpulan kawalan, TRF, FNT dan TRF+FNT. TRF (200 mg/kg bb) dan FNT (20 mg/kg bb) diberikan secara oral paksa selama 28 hari. Minyak jagung yang merupakan pengangkut telah diberikan secara oral kepada kumpulan kawalan. Pada akhir kajian, hepar dan darah diambil untuk menilai kerosakan oksidatif dan status biokimia serta pemerhatian histologi. Suplementasi TRF melindungi hepar daripada mengalami kerosakan oksidatif dengan menurunkan aras malondialdehid (MDA) dan protein karbonil (PCO) hepar secara signifikan. Tambahan pula, TRF juga mengembalikan aras antioksidan endogenus terutamanya glutation terturun (GSH), glutation peroksidase (GPx) dan pengurangan ferik /kuasa antioksida (FRAP). TRF juga berupaya melindungi hepar daripada mengalami kerosakan dengan merendahkan aras enzim hepar, alanine aminotransferae (ALT) dan aspartate aminotransferase (AST). Pengurangan kerosakan hepar tikus kumpulan TRF+FNT juga telah diperhatikan secara histologi. Kesimpulannya, suplementasi TRF berpotensi memberikan kesan perlindungan pada hepar tikus aruhan FNT dengan mengurangkan kerosakan oksidatif dan meningkatkan status antioksidan.

 

Keywords: Antioksida; minyak sawit; tekanan oksidatif; vitamin E

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*Corresponding author; email: balkis@ukm.edu.my