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Sains Malaysiana 47(10)(2018):
2337–2347 http://dx.doi.org/10.17576/jsm-2018-4710-10
Neuroprotective Effects of Ethyl Acetate Extract of Zingiber zerumbet (L.) Smith against Oxidative Stress on Paraquat-Induced Parkinsonism in Rats (Kesan Neurolindungi Ekstrak Etil Asetat Zingiber
zerumbet (L.)
Smith terhadap Tekanan Oksidatif pada Tikus Parkinsonisme Aruhan Parakuat) FARAH
WAHIDA
IBRAHIM,
UMI
NORAASHIKIN
ZAINUDIN,
MAZLYZAM
ABDUL
LATIF
& ASMAH HAMID* Biomedical Science Programme, Center for
Health & Applied Sciences, Faculty of Health Sciences, Universiti
Kebangsaan Malaysia, Jalan
Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Federal Territory, Malaysia Received: 14 March 2018/Accepted: 5 June 2018 ABSTRACT Zingiber zerumbet has been traditionally used as an anti-inflammation
and antioxidant agent. The present study investigates the neuroprotective
effects of ethyl acetate extract of Z. zerumbet against
oxidative stress on paraquat (PQ)-induced Parkinsonism in rats.
Forty male Sprague-Dawley rats were divided into five groups:
Negative control (normal saline), positive control (N-acetylcysteine,
NAC 20
mg/kg + PQ 10 mg/kg), PQ only, 200 mg/kg Z. zerumbet
+ PQ and 400 mg/kg Z. zerumbet + PQ.
The extract was given orally for 19 consecutive days and PQ was
administered intraperitoneally on day 8-12th of the treatment
regime. Both serum and fresh brains containing substantia nigra
(SN)
region were taken for biochemical and histological analysis. Administration
of both 200 and 400 mg/kg ethyl acetate Z.
zerumbet extracts to the PQ-treated groups have
resulted in: Decreased levels of MDA and PC in
the SN homogenates; and increased SOD,
GPx; and CAT activities in the SN
and serum. Overall, ethyl acetate extract of Z.
zerumbet reduced oxidative stress in the SN of PQ-induced neuronal damages, therefore,
has the potential to be developed as a preventive agent for neurodegenerative
disorders caused by environmental toxins. Keywords: Antioxidant;
oxidative stress; paraquat; Parkinson’s
disease; Zingiber zerumbet ABSTRAK Zingiber zerumbet
telah digunakan
secara tradisi
sebagai agen anti-keradangan dan antioksidan. Kajian ini dijalankan untuk mengkaji kesan neurolindungi ekstrak etil asetat
Z. zerumbet terhadap
tekanan oksidatif
dalam tikus Parkinsonisme
aruhan parakuat
(PQ).
Empat puluh
tikus Sprague-Dawley jantan dibahagikan kepada lima kumpulan: Kawalan negatif (salin normal), kawalan positif (N-asetilcysteine, NAC 20 mg/kg + PQ 10
mg/kg), PQ sahaja, 200 mg/kg Z. zerumbet + PQ dan
400 mg/kg Z. zerumbet + PQ. Ekstrak diberikan
secara oral gavaj
selama 19 hari berturut-turut dan PQ
diberikan secara intraperitoneal
pada hari ke-8 sehingga ke-12 rejim rawatan. Kedua-dua sampel serum dan
tisu otak
pada bahagian substantia nigra (SN) diambil
untuk analisis biokimia dan histologi.
Pemberian ekstrak
etil asetat Z. zerumbet pada kedua-dua dos 200
dan 400 mg/kg kepada kumpulan yang dirawat PQ
telah: Menurunkan tahap MDA dan PC
dalam homogenat SN;
meningkatkan aktiviti SOD,
GPx, dan CAT dalam SN dan serum;
serta meningkatkan
aras glutation
terturun (GSH) dalam
SN.
Secara
keseluruhannya, ekstrak etil asetat Z. zerumbet didapati
dapat mengurangkan
tekanan oksidatif pada kawasan SN di
otak tikus
yang diaruh kerosakan neuron oleh PQ. Seterusnya, berpotensi
untuk dibangunkan
sebagai agen pencegah
gangguan penyakit
neurodegeneratif yang disebabkan
oleh pendedahan terhadap toksin dari alam sekitar. Kata kunci: Antioksidan;
parakuat; penyakit
Parkinson; tekanan oksidatif;
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