Sains Malaysiana 47(4)(2018): 725-730

http://dx.doi.org/10.17576/jsm-2018-4704-10

 

Antioxidant and Mutagenic Activity of Herbal Tea Prepared from Cosmos caudatus Leaves at Different Maturity Stages

 

(Aktiviti Antioksida dan Mutagen di dalam Teh Herba daripada Daun Cosmos caudatus pada Tahap Kematangan Berbeza)

 

DIAN-NASHIELA FATANAH¹, NORIHAM ABDULLAH^1,2*, NOORAAIN HASHIM¹ & AZIZAH ABD. HAMID³

 

¹Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor Darul Ehsan, Malaysia

 

²Malaysia Institute of Transport (MITRANS), Universiti Teknologi MARA, 40450 Shah Alam, Selangor Darul Ehsan, Malaysia

 

³Faculty of Food Science and Technology Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

 

Received: 27 March 2017/Accepted: 26 October 2017

 

ABSTRACT

 

Different maturity stages of Cosmos caudatus leaves have been used to prepare herbal tea were investigated for their effect on antioxidant activity and mutagenic activity. The analyses carried out were total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), 2-2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, β-carotene bleaching assay, oxygen radical absorbance capacity (ORAC) and Ames Salmonella mutagenicity. The results demonstrated that, C. caudatus herbal tea prepared from young leaves showed significantly highest antioxidant activity for all assays tested, followed by mixed leaves, mature leaves, old leaves and the lowest was in C. caudatus herbal tea from a commercial brand. Pearson’s correlation coefficient also demonstrated that TPC and TFC displayed a strong correlation with all antioxidant activity assays, showing that these compounds were the major contributors to the antioxidant activity in C. caudatus herbal tea. However, all studied C. caudatus herbal tea showed no mutagenic effects against Salmonella typhimurium tester strain TA98 and TA100 with and without S9 metabolic activation. Hence, it can be concluded that, different maturity stages could affect the antioxidant activity in C. caudatus herbal tea as it reduced the antioxidant activity as maturity increased, but did not give any effect on the mutagenic activity.

 

Keywords: Antioxidant activity; Cosmos caudatus; herbal tea; maturity; mutagenic activity

 

 

ABSTRAK

 

Kesan aktiviti antioksida dan mutagen ke atas teh herba yang dihasilkan daripada daun Cosmos caudatus pada  tahap kematangan yang berbeza telah dikaji. Analisis yang dijalankan ialah jumlah kandungan fenolik (TPC), jumlah kandungan flavonoid (TFC), ujian penurunan ferik (FRAP), pemerangkapan radikal bebas 2-2-Difenil-1-pikrilhidrazil (DPPH), pelunturan β-karotena, kapasiti penyerapan radikal oksigen (ORAC) dan mutagenik Ames Salmonella. Keputusan menunjukkan bahawa, teh herba C. caudatus dihasilkan daripada daun muda mempunyai aktiviti antioksida yang tertinggi bagi kesemua asai yang diuji, diikuti oleh daun campuran, daun matang, daun tua dan nilai terendah diperoleh dari teh herba C. caudatus daripada jenama komersial. Pekali kolerasi Pearson juga memaparkan kolerasi yang kuat bagi TPC dan TFC dengan kesemua asai aktiviti antioksida yang dijalankan. Ini menunjukkan bahawa sebatian tersebut merupakan penyumbang utama kepada aktiviti antioksida di dalam teh herba C. caudatus. Walau bagaimanapun, kesemua teh herba C. caudatus yang dikaji tidak menunjukkan kesan mutagen terhadap strain penguji Salmonella typhimurium TA98 dan TA100 dengan dan tanpa pengaktifan metabolic S9. Sebagai kesimpulannya, peringkat kematangan daun yang berbeza boleh memberi kesan ke atas aktiviti antioksida di dalam teh herba C. caudatus, semakin meningkat kematangan daun yang digunakan, semakin rendah aktiviti antioksida, tetapi tiada kesan ke atas aktiviti mutagen.

 

Kata kunci: Aktiviti antioksida; aktiviti mutagen; Cosmos caudatus; kematangan; teh herba

 

 

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*Corresponding author; email: noriham985@salam.uitm.edu.my