Sains Malaysiana 47(8)(2018): 1787–1794

http://dx.doi.org/10.17576/jsm-2018-4708-17

Physicochemical and Structural Characterization of Surface Modified Electrospun PMMA Nanofibre

(Pencirian Fizikokimia dan Struktur bagi Permukaan Termodifikasi Elektroputaran Nanogentian PMMA)

RABIATUL ADAWIYAH RAZALI¹, YOGESWARAN LOKANATHAN¹, SHIPLU ROY CHOWDHURY¹, AMINUDDIN SAIM² & RUSZYMAH HAJI IDRUS^1,3*

¹Tissue Engineering Centre, Universiti Kebangsaan Malaysia (UKM) Medical Centre, Jalan Yaa'cob Latiff, 56000 Cheras, Kuala Lumpur, Federal Territory, Malaysia

²Ear, Nose and Throat Consultant Clinic, KPJ Ampang Puteri Specialist Hospital, Jalan Mamanda 9, Taman Dato Ahmad Razali, 68000 Ampang, Selangor Darul Ehsan, Malaysia

³Department of Physiology, Universiti Kebangsaan Malaysia, Jalan Yaa'cob Latiff, 56000 Cheras, Kuala Lumpur, Federal Territory, Malaysia

Received: 8 January 2018/Accepted: 19 April 2018

ABSTRACT

Although electrospun poly(methyl methacrylate) (PMMA) may mimic structural features of extracellular matrix, its highly hydrophobic nature causes reduced cell attachment. This study analysed the physicochemical and structural changes of the surface modified PMMA nanofiber. The electrospun PMMA nanofibers (PM) were surface-treated as follows: PM alone, collagen coated-PM (PM-C), UV-irradiated PM (PM-UV), collagen coated UV-irradiated PM (PM-C-UV) and collagen coated-PM crosslinked with genipin (PM-C-GEN). They were subjected to scanning electron microscopy, Fourier transform infrared (FTIR), cell attachment analysis, X-ray photoelectron spectroscopy (XPS), atomic force microscopy and X-ray powder diffraction (XRD). The surface roughness was lower in PM-C-UV group compared to others. Based on FTIR results, all expected functional group were present in all groups. XPS result showed that there are changes in the mass concentration of UV-treated surfaces and in the collagen coated surfaces. All PM groups showed amorphous nature through XRD. UV irradiation and collagen coating were shown to increase PM’s functional groups and modify its surface, which contributed to the increased attachment of cells onto the inert PM scaffold. As conclusion, collagen coated UV irradiated PMMA provided a better surface for cell to attach hence are suitable to be used further as scaffold for in vitro model.

Keywords: Electrospun nanofiber; PMMA; scaffold; surface modification; UV irradiation

ABSTRAK

Walaupun elektroputaran poli(metil metakrilat) (PMMA) boleh memimik sifat struktur matriks ekstrasel, ia terlalu hidrofobik lantas menyebabkan pengurangan pelekatan sel. Kajian ini telah menganalisis perubahan fizikokimia dan strukur permukaan termodifikasi nanogentian PMMA. Permukaan terawat nanogentian PMMA (PM) yang telah dielektroputar terbahagi seperti berikut: PM sahaja, PM bersalut kolagen (PM-C), PM diradiasi dengan UV bersalut kolagen (PM-UV), dan PM bersalut kolagen disilang dengan genipin (PM-C-GEN). Antara analisis yang dijalankan adalah mikroskopi elektron penskanan (SEM), inframerah transformasi Fourier (FTIR), analisis pelekatan sel, spektroskopi fotoelektron sinar-X (XPS), mikroskopi daya atom (AFM) dan pembelauan sinar-X (XRD). Kekasaran permukaan kumpulan PM-C-UV adalah kurang berbanding dengan yang lain. Berdasarkan hasil FTIR, semua kumpulan berfungsi yang dijangka wujud dalam semua kumpulan. Keputusan XPS menunjukkan bahawa terdapat perubahan dalam kepekatan jisim pada permukaan yang telah di UV radiasi dan yang telah disalut kolagen. XRD analisis menunjukkan semua kumpulan PM mempunyai sifat amorf. Sinar UV dan salutan kolagen telah menyebabkan peningkatan di dalam kumpulan berfungsi PM lantas mengubah suai permukaannya dan menyebabkan peningkatan pelekatan sel dalam perancah PM yang lengai. Kesimpulannya, PMMA diradiasi dengan UV bersalut kolagen adalah permukaan yang lebih baik untuk pelekatan sel dan menjadikannya sesuai untuk digunakan sebagai perancah model in vitro.

Kata kunci: Elektroputaran nanogentian; PMMA; perancah; permukaan terubah; sinar UV

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*Corresponding author; email: ruszyidrus@gmail.com