Sains Malaysiana 48(3)(2019): 523–531

http://dx.doi.org/10.17576/jsm-2019-4803-04

Structural Properties, Production, and Commercialisation of Invertase

(Sifat Struktur, Pengeluaran dan Pengkomersialan Invertase)

WEI CHENG PANG¹, AIZI NOR MAZILA RAMLI^1,2* & NUR DINI JOHARI¹

¹Faculty of Industrial Science & Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang Darul Makmur, Malaysia

²Bio Aromatic Research Centre of Excellence, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang Darul Makmur, Malaysia

Received: 30 April 2018/Accepted: 8 January 2019

ABSTRACT

The knowledge gained from yeast fermentation made invertase one of the earliest exploited enzymes in human history. Invertase functions as carbohydrases by hydrolysing sucrose into its simplest unit. Extensive studies on invertase have made it well-characterised through the discovery of its existence in a variety of living organisms. It is interesting to study the different types of invertase from either the same or different origins as they might have distinct properties and could possess unique characteristics. With the advancement in technology, the three-dimensional structure, catalytic domain, and mechanism of invertase action have been discovered. Furthermore, it is important to understand how this enzyme has been produced via fermentation or recombinant technology methods. Finally, invertase has been employed in several important industries and its future commercialisation is promising.

Keywords: Commercialisation; invertase; production; structural analysis

ABSTRAK

Pengetahuan yang diperoleh daripada penapaian ragi telah menjadikan invertase sebagai salah satu enzim yang paling awal dieksploitasi di dalam sejarah manusia. Invertase berfungsi sebagai karbohidrase dengan menghidrolisis sukrosa kepada glukosa dan fruktosa. Pelbagai kajian mengenai invertase dan penemuannya dalam pelbagai organisma telah mendalamkan pemahaman tentang invertase. Kajian mengenai invertase daripada punca yang sama atau berlainan adalah menarik kerana ia terdapat ciri-ciri yang berbeza dan unik. Dengan kemajuan bidang teknologi, struktur tiga dimensi, domain pemangkin dan mekanisme tindakan invertase telah dikaji. Tambahan pula, pengetahuan tentang bagaimana enzim ini dihasilkan melalui kaedah penapaian atau kaedah rekombinan juga sangat penting. Akhirnya, invertase telah digunakan dalam pelbagai sektor industri dan pengkomersilannya adalah sangat baik.

Kata kunci: Analisis struktur; invertase; pemasaran; pengeluaran

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*Corresponding author; email: aizinor@ump.edu.my