Sains Malaysiana 48(4)(2019): 851–860

http://dx.doi.org/10.17576/jsm-2019-4804-17

 

Green Synthesis of Ag Nanoparticles and Their Performance towards Antimicrobial Properties

(Sintesis Hijau Nanozarah Ag dan Prestasi ke Arah Sifat Antimikrob)

 

SUNDERISHWARY S MUNIANDY¹, S SASIDHARAN² & HOOI LING LEE¹*

 

¹Nanomaterials Research Group, School of Chemical Sciences, Universiti Sains Malaysia, 11800 Pulau Pinang, Malaysia

 

²Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Pulau Pinang, Malaysia

 

Received: 11 April 2018/Accepted: 19 January 2019

 

ABSTRACT

Green synthesis is a forthcoming trend in the nanotechnology field where classical methods of synthesis are now replaced by inexpensive and eco-friendly methods. In this study, a green method has been developed for the synthesis of silver nanoparticles (AgNPs) where AgNPs were synthesised using water-based facile hydrothermal method. Silver nitrate (AgNO3) and polyvinylpyrrolidone (PVP) were used as precursor and reducing agents to produce AgNPs. The molar ratio effect of the precursor and stabiliser, its reaction temperature and reaction time were investigated. X-ray Powder Diffraction (XRD), Field-Emission Scanning Electron Microscope (FESEM) and UV-Vis Spectrometry were used to characterise the AgNPs. The as-synthesized AgNPs with different molar ratios of the precursor to stabiliser were tested for antibacterial activities using Gram-positive bacteria (Bacillus subtilis) and Gram-negative bacteria (Escherichia coli). All the AgNPs samples exhibited antibacterial activities that were stronger against Gram-negative bacteria, as compared with Gram-positive bacteria. The diameter of the zone of inhibition (ZOI) increased with the increase of the AgNO3: PVP molar ratios. The results obtained proved that uniform AgNPs synthesized via green techniques have a high potential of influencing applications involving antimicrobial properties.

 

Keywords: Antibacterial activities; green synthesis; hydrothermal; nanoparticles; silver

 

ABSTRAK

Sintesis hijau adalah trend akan datang dalam bidang nanoteknologi yang menggantikan kaedah sintesis tradisi dengan kaedah yang mesra alam dan murah. Dalam kajian ini, kaedah hijau telah dibangunkan untuk mensintesis nanozarah perak (AgNPs) dengan AgNPs disintesis menggunakan air-berdasarkan kaedah reaksi mudah hidroterma. Perak nitrat (AgNO3) dan polivinilpirolidon (PVP) telah digunakan sebagai pelopor dan ejen penurunan untuk menghasilkan AgNPs. Kesan nisbah molar pelopor dan penstabil, suhu tindak balas dan masa tindak balas telah dikaji. Ujian X-Ray Pembelauan Serbuk (XRD), Pancaran Medan Mikroskop Elektron Pengimbas (FESEM) dan Spektroskopi UV-Vis digunakan untuk mencirikan AgNPs. AgNPs disintesis dengan nisbah molar yang berbeza daripada pelopor dan penstabil telah diuji untuk aktiviti antibakteria menggunakan bakteria Gram-positif (Bacillus subtilis) dan bakteria Gram-negatif (Escherichia coli). Semua sampel AgNPs menunjukkan aktiviti anti-bakteria yang kuat terhadap bakteria Gram-negatif, berbanding dengan bakteria Gram-positif. Zon diameter perencatan (ZOI) meningkat dengan peningkatan nisbah molar AgNO3: PVP. Keputusan yang diperoleh membuktikan bahawa AgNPs berseragam yang disintesis melalui teknik hijau berpotensi tinggi untuk mempengaruhi aplikasi yang melibatkan sifat antimikrob.

 

Kata kunci: Aktiviti anti-bakteria; hidroterma; nanozarah; perak; sintesis hijau

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*Corresponding author; email: hllee@usm.my