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Sains Malaysiana 48(5)(2019): 1105–1110 http://dx.doi.org/10.17576/jsm-2019-4805-19
Optimization of Cryopreserved Single Cell
Suspension of Gastric Epithelial Cells for Successful DotScanTM
Antibody Microarray (Pengoptimuman
Pengkrioawetan
Ampaian Sel Tunggal Sel Epitelium Gaster bagi Kejayaan Mikroatur
Antibodi DotScanTM) ASIF SUKRI1,
ALFIZAH
HANAFIAH1*,
NIK
RITZA
KOSAI2,
MUSTAFA
MOHAMED
TAHER2,
ISA MOHAMED
ROSE3
& RAMELAH MOHAMED4 1Department of Medical Microbiology
and Immunology, Faculty of Medicine, Universiti
Kebangsaan Malaysia, Jalan Yaacob
Latiff, Bandar Tun
Razak, 56000 Cheras, Kuala Lumpur,
Federal Territory, Malaysia 2Department of Surgery, Faculty of
Medicine, Universiti Kebangsaan Malaysia,
Jalan Yaacob Latiff,
Bandar Tun Razak,
56000 Cheras, Kuala Lumpur, Federal Territory, Malaysia 3Department of Pathology, Faculty
of Medicine, Universiti Kebangsaan
Malaysia, Jalan Yaacob Latiff,
Bandar Tun Razak,
56000 Cheras, Kuala Lumpur, Federal Territory, Malaysia 4Biotech Diagnostics, UKMTech, Universiti Kebangsaan Malaysia,
43600 UKM Bangi, Selangor Darul Ehsan, Malaysia Received:
9 September 2016/Accepted: 5 March 2019 ABSTRACT Cell cryopreservation plays
a pivotal role for successful immunological assays. DotScanTM antibody
microarray employs live cells with satisfactory cell viability
for detection of clusters of differentiation (CD) antigens in various diseases.
Effective cryopreservation contributes to successful capture
of cells on DotScanTM antibody
microarray. The purpose of this study was to evaluate the effect
of cells viability on cells binding to specific CD antigens. Cells with similar
concentration but different percentage of cell viabilities were
captured on DotScanTM slides.
Cells stored for 5 days at -80ºC and added with freezing medium
in stepwise manner had higher viabilities as compared to cells
stored for 6 months at -80ºC and added with freezing medium
in direct manner (p=0.012).
Cells with 88% viability had higher binding to CD44/CD29
control antibodies as compared to cells with 33% viability.
Short-term storage of cells and the technique for freezing medium
addition to cells play a critical role for successful cryopreservation
of single cell suspension. Keywords: Cell surface antigens;
cryopreservation; DotScanTM antibody
microarray; epithelial cells ABSTRAK Pengkrioawetan sel memainkan peranan
penting dalam
kejayaan asai imunologi.
Mikroatur antibodi
DotScanTM memerlukan sel hidup yang memuaskan untuk pengesanan antigen kluster pembezaan (CD)
dalam pelbagai
penyakit. Keberkesanan pengkrioawetan menyumbang kepada kejayaan penangkapan sel ke atas mikroatur
antibodi DotScanTM.
Tujuan kajian
ini adalah untuk
menilai kesan
daya hidup sel
ke atas
pelekatan sel
terhadap antigen CD
yang khusus. Sel
dengan kepekatan yang sama tetapi daya
hidup yang berlainan
ditangkap ke atas
slaid DotScanTM.
Sel disimpan
selama 5 hari pada suhu -80°C dan ditambah media takat beku secara berperingkat
mempunyai daya
hidup yang lebih tinggi berbanding sel yang disimpan selama 6 bulan dan ditambah media takat beku secara
langsung (p=0.012). Sel dengan daya
hidup 88% mempunyai
pelekatan yang lebih tinggi terhadap antibodi kawalan CD44/CD29
berbanding sel
dengan daya hidup
33%. Sel yang disimpan
untuk jangka masa singkat dan teknik
menambah media takat
beku terhadap sel
memainkan peranan
penting untuk kejayaan
pengkrioawetan ampaian sel tunggal. Kata kunci: Antigen permukaan
sel; mikroatur
antibodi DotScanTM;
pengkrioawetan; sel
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