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Sains Malaysiana 48(5)(2019): 937–944 http://dx.doi.org/10.17576/jsm-2019-4805-02
Effects of Silver Nanoparticle Exposure
on Germination and Early Growth of Pinus
sylvestris and Alnus
subcordata (Kesan Pendedahan Nanozarah Perak kepada Percambahan dan Pertumbuhan Awal Pinus sylvestris dan Alnus subcordata) VILMA BAYRAMZADEH1*,
MARYAM GHADIRI2
& MOHAMMAD HOSSEIN DAVOODI3 1Department of Wood
Sciences Faculty of Agricultural Sciences and Natural Resources,
Karaj Branch, Islamic Azad University, Karaj, Iran 2M.Sc. Department
of Soil Sciences, Faculty of Agricultural Sciences and Natural
Resources, Karaj Branch, Islamic Azad University, Karaj, Iran 3Soil and Water
Research Institute, Karaj, Iran Received: 9 June 2018/Accepted:
12 March 2019 ABSTRACT The possible ecological toxicity
of silver nanoparticles (AgNP) was
evaluated based on germination and growth characteristics of
Pinus sylvestris and Alnus
subcordata. Seeds were exposed
to different concentrations of AgNP
in soil (0, 10, 20, 40, 80 and 100 mg/kg) and aqueous suspension
(0, 10 and 20 mg/L). Then, seed germination percentage (GP%),
speed of germination (S.G), seedling length (SL),
as well as fresh and dry weights (FW and DW)
were measured. The results showed that low concentration of
AgNP (under 80 mg/kg) could be used
without detrimental effects on the germination characteristics
of P. sylvestris in the soil.
Nevertheless, inhibitory effect of AgNP
was observed at 10 mg/L for P. sylvestris
in aqueous suspension. The dissimilar results in soil and
aqueous suspension were due to the organic matters and clay
minerals in the soil. There was no significant difference (p<0.01)
among the treatments of A. subcordata, not only in soil but also in the aqueous suspension. Keywords: Aqueous suspension;
inhibitory effect; seed germination; silver nanoparticles; soil;
woody plants ABSTRAK Ketoksikan ekologi nanozarah perak (AgNP) dinilai
berdasarkan ciri-ciri
percambahan dan pertumbuhan Pinus sylvestris dan Alnus subcordata. Benih didedahkan kepada kepekatan berbeza AgNP dalam
tanah (0, 10, 20, 40, 80 dan
100 mg/kg) dan gantungan
akues (0, 10 dan 20 mg/L). Kemudian, peratusan percambahan biji benih (GP%), kelajuan percambahan (S.G), panjang semaian (SL), serta
berat kering
dan segar (FW dan DW) diukur.
Hasil kajian menunjukkan
bahawa kepekatan
rendah AgNP (di bawah 80 mg/kg) boleh digunakan tanpa kesan-kesan yang memudaratkan terhadap ciri-ciri percambahan P. sylvestris dalam tanah. Walau bagaimanapun, kesan rencatan AgNP diperhatikan pada 10 mg/L bagi P. sylvestris pada penggantungan akues. Keputusan yang berbeza dalam tanah dan
penggantungan akues
adalah disebabkan oleh bahan organik
dan mineral tanah
liat dalam tanah.
Tiada perbezaan
yang signifikan (p<0.01) bagi
rawatan A. subcordata,
di dalam tanah
mahupun di dalam penggantungan akues. Kata kunci: Kesan
rencatan; penggantungan
akues; percambahan benih; nanozarah perak; tanah; tumbuhan
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