Sains Malaysiana 49(5)(2020): 989-994

http://dx.doi.org/10.17576/jsm-2020-4905-03

Cytotoxic Triterpenoids from the Stem Barks of Dysoxylum arborescensand Dysoxylum excelsum against MCF-7 Breast Cancer Cell

(Triterpenoid Sitotoksik daripada Kulit Batang Dysoxylum arborescens dan Dysoxylum excelsum terhadap Sel Kanser Payudara MCF-7)

ACHMAD ZAINUDDIN¹, SYLVIA RACHMAWATI MEILANIE¹, DARWATI¹, KURNIAWAN¹, NURLELASARI¹, TATI HERLINA¹, ADRIAN RIZKY SAPUTRA¹, JAMALUDIN AL ANSHORI^1,2 & TRI MAYANTI¹*

¹Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km 21 Jatinangor 45363, Indonesia

²Laboratory of Applied Chemistry and Services, Faculty of Mathematics and Natural Sciences

Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km 21 Jatinangor 45363, Indonesia

Received: 11 February 2019/Accepted: 19 January 2020

ABSTRACT

Three triterpenoids, taraxerone (1) and 18-epi-taraxerol (2), and masticadienolic acid (3) were isolated for the first time from the stem barks of Dysoxylum arborescens and Dysoxylum excelsum, respectively. Isolation of the compounds was achieved by various extraction and chromatography column techniques, while their chemical structures were elucidated based on spectroscopic data and literature comparison. Compounds 1, 2, and 3 were found to show inhibitory activity against MCF-7 breast cancer cells with IC₅₀ values 792.9, 59.6, and 3.5 µM correspondingly.

Keywords: Dysoxylum arborescens; Dysoxylum excelsum; MCF-7; triterpenoids

ABSTRAK

Tiga triterpenoid, tarakseron (1) dan 18-epi-tarakserol (2), asid mastikadienolat (3) telah dipisahkan daripada kulit batang Dysoxylum excelsum dan Dysoxylum arborescens buat pertama kali. Sebatian 1, 2 dan 3 dipencilkan menggunakan pelbagai teknik lajur ektraksi dan kromatografi, sementara penentuan strukturnya menggunakan bantuan data spektroskopi dan perbandingan dengan sebatian yang pernah dilaporkan sebelumnya. Aktiviti sitotoksik sebatian 1, 2 dan 3 terhadap sel kanser payudara MCF-7 menunjukkan perencatan dengan nilai IC₅₀ masing-masing 792.9, 59.6 dan 3.5 µM.

Kata kunci: Dysoxylum arborescens; Dysoxylum excelsum; MCF-7; triterpenoid

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*Corresponding author; email: t.mayanti@unpad.ac.id