Sains Malaysiana 49(6)(2020): 1237-1244

http://dx.doi.org/10.17576/jsm-2020-4906-03

Effect of Lactobacillus casei and Lactobacillus acidophilus in Laying Hens Challenged by Escherichia coli Infection

(Kesan Lactobacillus casei dan Lactobacillus acidophilus dalam Ayam Bertelur yang Dijangkiti Escherichia coli)

WIDYA PARAMITA LOKAPIRNASARI¹*, ADRIANA MONICA SAHIDU², LILIK MASLACHAH³, EMY KOESTANTI SABDONINGRUM¹ & ANDREAS BERNY YULIANTO⁴

¹Department of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Mulyorejo, Kampus C, Surabaya, 60115, Indonesia

²Department of Marine, Faculty of Fisheries and Marine, Universitas Airlangga, Jl. Mulyorejo, Kampus C, Surabaya, 60115, Indonesia

³Department of Basic Medicine, Veterinary Pharmacy Laboratory, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Mulyorejo, Kampus C, Surabaya, 60115, Indonesia

⁴Sains Veteriner, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Mulyorejo, Kampus C, Surabaya, 60115, Indonesia

Received: 6 April 2019/Accepted: 14 February 2020

ABSTRACT

This study aimed to prove the potential of Lactobacillus casei and Lactobacillus acidophilus probiotics as alternative substitutes of antibiotic growth promoters in laying hens challenged by Escherichia coli infection in order to enhance their growth performance and hen day production. The study used a total of 120 laying hens aged 25 weeks, divided into 3×2 treatments with each comprising 4 replications, and each replication consisted of 5 hens. The study used a completely randomised factorial design; factor a was the feed additive (control, antibiotics growth promoters /AGP, probiotic), whereas factor b was the E. coli infection (non-infection and E. coli infection). The results showed that there were significant differences (p<0.05) between the treatment of feed additive (factor a), and E. coli infection (factor b), and interaction (p<0.05) between the feed and the infection for the egg weight, hen day production, feed conversion ratio and feed efficiency. The probiotic use of 0.5% L. casei + 0.5% L. acidophilus in hens either infected or uninfected with E. coli still produced the highest egg weight, hen day production, feed efficiency and reduced feed conversion ratio compared to all treatments. Based on the results, it can be concluded that the use of probiotics 0.5% L. casei and 0.5% L. acidophilus act as alternative substitutes for antibiotic growth promoters in laying hens challenged by E.  coli infection.

Keywords: E. coli; growth performance; hen day production; Lactobacillus acidophilus; Lactobacillus casei

ABSTRAK

Kajian ini bertujuan membuktikan potensi probiotik Lactobacillus casei dan Lactobacillus acidophilus sebagai pengganti alternatif penggalak pertumbuhan antibiotik (AGP) dalam ayam bertelur yang dijangkiti Escherichia coli terhadap prestasi pertumbuhan dan pengeluaran telur harian. Kajian ini menggunakan 120 ekor ayam bertelur berumur 25 minggu yang dibahagikan kepada rawatan 3×2, dengan setiap rawatan terdiri daripada 4 replikasi dan setiap replikasi terdiri daripada 5 ekor ayam bertelur. Kajian ini menggunakan reka bentuk faktoran rawak lengkap; faktor a adalah makanan tambahan (kawalan, AGP, probiotik), manakala faktor b adalah faktor jangkitan E. coli (bukan jangkitan dan jangkitan E. coli). Hasil kajian menunjukkan terdapat perbezaan yang signifikan (p<0.05) antara rawatan aditif makanan (faktor a) dan rawatan jangkitan E. coli (faktor b), serta interaksi antara faktor makanan tambahan dan jangkitan pada berat telur, pengeluaran telur harian, nisbah penukaran makanan dan kecekapan suapan. Penggunaan probiotik 0.5% L. casei + 0.5% L. acidophilus dalam ayam sama ada dijangkiti atau tidak dijangkiti E. coli masih memberikan berat telur tertinggi, pengeluaran telur harian dan kecekapan suapan yang tertinggi dan menurunkan nisbah penukaran makanan berbanding semua rawatan. Berdasarkan hasil kajian, dapat disimpulkan bahawa penggunaan probiotik 0.5% L. casei + 0.5% L. acidophilus dapat digunakan sebagai pengganti alternatif bagi promoter pertumbuhan antibiotik dalam ayam petelur yang dijangkiti E. coli.

Kata kunci: E. coli; Lactobacillus acidophilus; Lactobacillus casei; pengeluaran telur harian; prestasi pertumbuhan  

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*Corresponding author; email: widya-p-l@fkh.unair.ac.id