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Sains Malaysiana 49(6)(2020): 1303-1312
http://dx.doi.org/10.17576/jsm-2020-4906-08
Anti-inflammatory Effect of Tamarind Seed
Coat Extract against LPS-Induced RAW264.7 Macrophages
JIRAPA PUNTARUT1,
WIPAWADEE SIANGLUM2, SUPITA TANASAWET3, PENNAPA
CHONPATHOMPIKUNLERT4 & WANIDA SUKKETSIRI1*
1Department
of Pharmacology, Faculty of Science, Prince of Songkla University, Songkhla, 90110 Thailand
2Department
of Microbiology, Faculty of Science, Prince of Songkla University, Songkhla, 90110
Thailand
3Department
of Anatomy, Faculty of Science, Prince of Songkla University, Songkhla, 90110, Thailand
4Expert Center
of Innovative Health Food, Thailand Institute of Scientific and Technological
Research (TISTR), Pathumthani, 12120, Thailand
Received: 2 August 2019/Accepted:
17 February 2020
ABSTRACT
Inflammatory
response is modulated by stimulated immune cells, and
has a pivotal role in host defense system against various stimuli. In this study, we evaluated the anti-inflammatory property
of tamarind seed coat extract (TSCE) in lipopolysaccharide (LPS)-induced
RAW264.7 macrophages. Various concentrations of TSCE (10, 25, and 50 µg mL-1) were applied and
then stimulated with LPS (1 μg mL-1)
in RAW264.7 macrophages and the level of reactive oxygen species (ROS) and nitric oxide (NO) were
measured. Besides, enzyme-linked immunosorbent assay (ELISA) was
used to measure the level of pro-inflammatory cytokines. Our results
showed that TSCE suppressed LPS-induced
intracellular ROS production and suppressed the NO levels in a dose-dependent
manner. Significantly, the anti-inflammatory activity was correlated with a
lowered LPS-stimulated TNF-α and IL-1β pro-inflammatory cytokines.
These results implied that TSCE possess potent
anti-inflammatory activity, which supported new insights into the TSCE
utilization to protect inflammation-related disorders.
Keywords: Interleukin-1β; reactive
oxygen species; Tamarindus indica; tumor necrosis factor alpha
ABSTRAK
Tindak balas keradangan dimodulasi oleh sel-sel
imun yang dirangsang dan memainkan peranan penting dalam sistem pertahanan
perumah terhadap pelbagai rangsangan. Dalam kajian ini, kami menilai sifat
anti-radang daripada ekstrak lapisan biji asam jawa (TSCE) pada
lipopolisakarida (LPS) yang diaruh makrofaj RAW264.7. Pelbagai kepekatan TSCE
(10, 25 dan 50 µg mL-1) digunakan dan kemudian dirangsang dengan LPS
(1 μg mL-1) pada makrofaj RAW264.7 dan tahap spesies
oksigen reaktif (ROS) dan nitrik oksida (NO) diukur. Selain itu, asai
imunosorben berkait enzim (ELISA) digunakan untuk mengukur tahap sitokin
pro-radang. Hasil kajian menunjukkan bahawa TSCE menindas pengeluaran
intraselular ROS yang disebabkan oleh LPS serta menindas aras NO bergantung
pada dos atau kepekatan. Aktiviti anti-radang berkorelasi dengan penurunan LPS
yang merangsang sitokin pro-radang TNF-α dan IL-1β secara ketara.
Hasil ini menunujukkan bahawa TSCE memiliki aktiviti anti-radang yang tinggi
sekaligus menyokong pandangan baru tentang penggunaan TSCE dalam melindungi
gangguan yang berkaitan dengan keradangan.
Kata kunci: Faktor nekrosis tumor alfa;
interleukin-1β; spesies oksigen reaktif; Tamarindus indica
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*Corresponding author; email: wanida.su@psu.ac.th
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