Sains Malaysiana 49(9)(2020): 2231-2236

http://dx.doi.org/10.17576/jsm-2020-4909-20

Preparation and Characterization of Chitosan-Coated Oleic Acid Liposomes for Intravenous Delivery

(Penyediaan dan Pencirian Liposom Asid Oleik Bersalut Kitosan untuk Penghantaran Intravena)

NOOR ASHYFIYAH BASROWI, VICIT RIZAL EH SUK, RAHADIAN PERMADI & MISNI MISRAN*

Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Federal Territory, Malaysia

Received: 15 October 2019/Accepted: 8 May 2020

ABSTRACT

Liposome has been studied as a potential carrier for targeting and controlled drug delivery. However, poor stability remains a challenge because it can lead to drug leakage from the vesicles thus reduce the effectiveness towards the target cell. For this aim, the present study incorporated the low molecular weight chitosan (LMWC) into the oleic acid liposome to maintain the stability and prolong the lifetime in the blood circulation. The thin-film hydration method was employed to prepare the oleic acid liposomes prior to coating them with LMWC. The stability of the liposomes was determined by the measurement of particle size and zeta potential for 28 days. The morphology of the liposome was confirmed by observing the shape under transmission electron microscopy (TEM) and it showed almost spherical in shape. The average particle size increased to 201.23 nm and -51.4 mV when 5 mg of LMWC was added to the oleic acid liposome. The increase of particle size and zeta potential of LMWC-coated liposome indicated that polymer-liposome interaction had changed the stability of liposome thus this invention could be useful for delivering active ingredients through intravenous delivery.

Keywords: Itraconazole; liposome; low molecular weight; oleic acid

ABSTRAK

Liposom sedang dikaji sebagai sebuah pengangkut yang berpotensi tinggi bagi penyasaran dan pengawalan penghantaran ubat. Walau bagaimanapun, mengekalkan kestabilannya merupakan satu cabaran kerana ia boleh menyebabkan ubat terkeluar dari vesikel serta mengurangkan keberkesanan terhadap sel yang hendak disasarkan. Kajian ini bermatlamat untuk menyalutkan kitosan berjisim molekul rendah ke dalam liposom asid oleik untuk mengekalkan kestabilan dan jangka hayatnya dalam peredaran darah. Kaedah penghidratan lapisan nipis digunakan untuk menghasilkan liposom asid oleik sebelum disalut dengan kitosan berjisim molekul rendah. Kestabilan liposom ditentukan dengan mengukur saiz zarah dan potensi zeta selama 28 hari. Morfologi liposom ditentukan dengan memerhatikan bentuk di bawah mikroskop elektron transmisi dan menunjukkan bentuk seakan bentuk sfera. Purata saiz zarah meningkat kepada 201.23 nm dan -51.4 mV apabila 5 mg kitosan telah ditambahkan ke liposom asid oleik. Peningkatan saiz zarah dan potensi zeta bagi liposom bersalut kitosan berjisim molekul rendah menunjukkan bahawa interaksi polimer dan liposom telah mengubah kestabilan liposom dan penemuan ini berpotesi untuk menyampaikan bahan aktif secara penghantaran intravena.

Kata kunci: Asid oleik; Itraconazole; kitosan berjisim molekul rendah; liposom

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*Corresponding author; email: misni@um.edu.my