Sains Malaysiana 50(3)(2021): 723-733

http://doi.org/10.17576/jsm-2021-5003-14

Artificial Cultivation Anti-tumor Activity of Ganoderma mbrekobenum

(Penanaman Buatan dan Aktiviti Anti-barah Ganoderma mbrekobenum)

HU HUIPING¹*, LIU YUANCHAO¹, LIANG XIAOWEI¹, LI XIANGMIN¹, MO WEIPENG¹, XIE YIZHEN^1,2, ZHANG ZHI² & WU QINGPING¹

¹Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangdong 510070, Guangzhou, China

²Guangdong Yuewei Edible Fungi Technol. Co. Ltd., Guangdong 510663, Guangzhou, China

Received: 25 December 2019/Accepted: 26 August 2020

ABSTRACT

Different varieties of Gandoerma have different functional effects. In this study, identification, cultivation, anti-tumor activity characterization and active constituent determination of Ganoderma specimen collected in Tanzania were carried out. The fungi specimen was identified as Ganoderma mbrekobenum by morphological and molecular methods, which was found in Ghana and was firstly reported in 2016. The fruit body was formed in artificial culture using sawdust as the main substrate at the suitable fruiting temperature of 27-30 ℃, relative humidity 85-90%, and daily processed lighting for 10 h (300-500 Lux). The fruit body of G. mbrekobenum is hard and has ordinary-yield production, uniform shapes, and high stability. The in vitro experiments showed that the extract from G. mbrekobenum fruit body inhibited cancer cell proliferation of various cells, such as HepG2, MDA-MB-231 and U87. The water extract of the fruit body was tested, and the content of crude polysaccharides was 1.12%. The data showed that G. mbrekobenum was a potential valuable variety. This is the first study that reports the cultivation method and the anti-tumor activity of G. mbrekobenum.

Keywords: Ganoderma; medical fungi; polysaccharides

ABSTRAK

Varieti Ganoderma yang berbeza mempunyai kesan kefungsian yang berbeza. Dalam kajian ini, pengenalpastian, penanaman, pencirian aktiviti anti-barah dan penentuan sebatian aktif terhadap spesimen Ganoderma yang diperoleh dari Tanzania telah dijalankan. Melalui kaedah morfologi dan molekul, spesimen kulat tersebut telah dikenal pasti sebagai Ganoderma mbrekobenum, yang sebelum ini ditemui di Ghana dan pertama kali dilaporkan pada tahun 2016. Jasad buah telah terhasil dalam kultur buatan menggunakan serbuk gergaji sebagai substrat utama pada suhu untuk berbuah yang sesuai antara 27-30 °C, kelembapan relatif 85-90% dan pencahayaan setiap hari selama 10 jam (300-500 Lux). Jasad buah G. mbrekobenum adalah keras dan mempunyai penghasilan biasa, berbentuk seragam dan kestabilan yang tinggi. Uji kaji secara in vitro menunjukkan bahawa ekstrak daripada jasad buah G. mbrekobenum merencat pembahagian beberapa sel barah seperti HepG2, MDA-MB-231 dan U87. Ekstrak air jasad buah telah diuji dan didapati bahawa kandungan polisakarida mentah adalah sebanyak 1.12%. Data menunjukkan bahawa kulat ini merupakan varieti bernilai yang berpotensi. Ini merupakan kajian yang pertama melaporkan kaedah penanaman dan aktiviti anti-barah G. mbrekobenum.

Kata kunci: Ganoderma; kulat perubatan; polisakarida

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*Corresponding author; email: huhp@gdim.cn