Sains Malaysiana 50(4)(2021): 897-905

http://doi.org/10.17576/jsm-2021-5004-02

Anatomi dan Mikromorfologi Tetrastigma rafflesiae (Vitaceae)

(Anatomy and Micromorphology of Tetrastigma rafflesiae (Vitaceae))

SYAMSURINA ARSHAD, MOHD AFIQ AIZAT JUHARI*, NORAINI TALIP, NOR AZILAH ABDUL WAHAB & JUMAAT ADAM

Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia, 43650 UKM Bangi, Selangor Darul Ehsan, Malaysia

Received: 18 September 2018/Accepted: 2 September 2020

ABSTRAK

Kajian anatomi dan mikromorfologi telah dilakukan ke atas Tetrastigma rafflesiae (Miq.) Planchon, perumah kepada bunga pakma di Perak, Pahang dan Kelantan. Penglibatan kajian ini merangkumi anatomi keseluruhan tumbuhan ini, iaitu bahagian daun, batang, sulur paut dan akar kerana kajian anatomi masih dangkal di Malaysia amnya dan dunia khasnya. Sampel tumbuhan ini diperoleh dari Sungai Bongor, Grik, Perak, Sungai Yol, Raub, Pahang dan Lojing, Kelantan. Teknik piawai dalam anatomi tumbuhan digunakan dan ini melibatkan penggunaan mikroskop cahaya dan mikroskrop imbasan elektron (SEM). Hasil kajian anatomi menunjukkanT. rafflesiae mempunyai corak bekas vaskular dengan sistem tertutup pada petiol, tulang daun, batang dan akar; hablur didapati hadir pada petiol, tulang daun, lamina, peruratan, batang dan akar dan dinding antiklin abaksial dan adaksial adalah daripada lurus ke melengkung. Hasil mikromorfologi memperlihatkan spesies ini mempunyai stomata homostomatik dan hipostomatik daripada jenis diasitik. Lilin epikutikel berbentuk lembaran dan lapisan filem pada bahagian adaksial manakala berbentuk granul, lapisan filem dan lembaran pada bahagian abaksial. Hasil kajian ini dapat digunakan sebagai perintis kepada kajian anatomi spesiesTetrastigma yang lain.

Kata kunci: Anatomi; mikromorfologi; Tetrastigma rafflesiae

ABSTRACT

Anatomy and micromorphology study were undertaken on Tetrastigma rafflesiae (Miq.) Planchon, the host of Rafflesia flower in Peninsular Malaysia. The assessment included all the anatomy parts of this plant (leaves, stems, tendrils and roots) because the anatomy study of this plant still lacking in Malaysia and also the entire world. The plant samples were collected from Sungai Bongor, Grik, Perak, Sungai Yol, Raub, Pahang and Lojing, Kelantan. Standard techniques in plant anatomy were employed, including analyses under light microscopy and scanning electron microscopy (SEM). Anatomy results showed that T. rafflesiae has close vascular bundle system at petiole, midrib, stems and root; druses are present at petioles, midribs, laminas, veins, stem and roots; abaxial and adaxsial anticlinal walls are straight to curve. Micromorphology results showed that this species has homostomatic and hypostomatic stomata of diacytic type. Epicuticular wax is in layers and films at adaxial, and in granule, films and layers at abaxial. This study can be used as a pioneer for the anatomy investigation of other Tetrastigma species.

Keywords: Anatomy, micromorphology, Tetrastigma rafflesiae

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*Corresponding author; email: afiqaizat@ukm.edu.my