Sains Malaysiana 50(5)(2021): 1277-1284

http://doi.org/10.17576/jsm-2021-5005-08

The Effect of MS Media Strength and Cytokinin in the Induction of Shoots from Shoot Tip Explants of Australian Finger Lime (Citrus australasica cv. Tasty Green)

(Kesan Kekuatan Medium MS dan Sitokinin dalam Aruhan Pucuk daripada Hujung Pucuk Eksplan Limau Jejari Australia (Citrus australasica cv. Tasty Green))

DAHMENDRA SRISKANDA¹, KHOY SIONG POI¹, NAJWA AMALINA HARADZI¹, NURULHIKMA MD ISA², SREERAMANAN SUBRAMANIAM¹ & BEE LYNN CHEW¹*

¹School of Biological Sciences, Universiti Sains Malaysia, 11800 Pulau Pinang, Malaysia

²Department of Biological Sciences & Biotechnology, Faculty of Science & Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia

Received: 19 February 2020/Accepted: 8 October 2020

ABSTRACT

The Australian Finger Lime (Citrus australasica) is a type of citrus from the Rutaceae family, endemic to the east coast of Australia. The finger lime, loaded with numerous vitamins and renders a unique taste, has also been backed by science to contain essential amounts of antioxidants that are beneficial for cell protection, immune response, cancer prevention, ageing, arthritis and prevention of kidney stones. Current propagation attempts still rely on conventional methods that are less efficient and resulted in the slow establishment of farms for finger lime especially for commercialization purposes. This study focuses on the induction of shoots from shoot tip explants using 6-Benzylaminopurine (BAP) and Kinetin. Aseptic explants were inoculated into Murashige and Skoog (MS) medium of full-strength and half-strength followed by full-strength MS media supplemented with different concentrations of BAP and Kinetin. Results obtained in this study showed no significant differences in terms of the number of axillary shoots produced between explants cultured in full and half-strength MS media. However, the highest number of shoots and increment in shoot length were obtained from MS media supplemented with 2.0 mg/L BAP with the values 1.80 ± 0.27 and 2.56 ± 0.36 cm, respectively. In conclusion, MS media supplemented with 2.0 mg/L BAP was found optimal in the induction of shoots and shoot elongation of C. australasica cv. Tasty Green.

Keywords: 6-Benzylaminopurine; Australian Finger Lime; axillary shoots; Citrus australasica; kinetin

ABSTRAK

Citrus australasica yang dikenali sebagai Limau Jejari Australia adalah daripada famili Rutaceae yang biasanya terdapat di pantai timur Australia. Limau ini kaya dengan pelbagai jenis vitamin dan mempunyai rasa yang unik, serta telah terbukti secara saintifik mempunyai kandungan antioksida yang penting untuk perlindungan sel, tindak balas imun, perlindungan terhadap kanser, penuaan, artritis dan pencegahan batu karang. Propagasi pokok limau ini masih bergantung pada kaedah konvensional yang kurang cekap dan menyukarkan penubuhan ladang limau ini terutamanya untuk tujuan komersial. Kajian semasa memfokuskan kepada penginduksian pucuk daripada eksplan pucuk menggunakan Benzilaminopurina (BAP) dan Kinetin. Eksplan steril dikultur dalam media Murashige dan Skoog (MS) pada kekuatan penuh dan separuh, serta MS berkekuatan penuh dengan penambahan BAP dan Kinetin. Keputusan daripada kajian ini menunjukkan tiada perbezaan yang signifikan dari segi jumlah pucuk aksilari yang dihasilkan antara eksplan yang dikultur dalam media MS kekuatan penuh dan separuh. Walau bagaimanapun, bilangan pucuk dan pertambahan ketinggian eksplan diperoleh dalam media MS pada kekuatan penuh ditambah dengan 2.0 mg/L BAP dengan nilai masing-masing 1.80 ± 0.27 dan 2.56 ± 0.36 cm. Kesimpulannya, media MS yang ditambah dengan 2.0 mg/L BAP didapati lebih optimum daripada rawatan yang lain dalam penginduksian pucuk dan pemanjangan pucuk C. australasica cv. Tasty Green.

Kata kunci: 6-benzilaminopurina; Citrus australasica; kinetin; Limau Jejari Australia; pucuk aksilari

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*Corresponding author; email: beelynnchew@usm.my