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Sains
Malaysiana 51(1)(2022): 187-198
http://doi.org/10.17576/jsm-2022-5101-15
Pleurotus pulmonarius (Fr.) Quel Crude Aqueous Extract Ameliorates Wistar-Kyoto Rat Thoracic Aortic
Tissues and Vasodilation Responses
(Pleurotus pulmonarius (Fr.) Quel Ekstrak
Akueus Mentah dalam Memperbaiki Tisu Toraks Aorta dan Respons Vasodilatasi
Tikus Wistar-Kyoto)
NOOR FAZILA MOHD YAHAYA, NORHANIZA
AMINUDIN* & NOORLIDAH ABDULLAH
Mushroom Research Centre, Institute
of Biological Sciences, Faculty of Science, Universiti Malaya, 50603 Kuala
Lumpur, Federal Territory, Malaysia
Received: 15 January 2021/Accepted:
18 May 2021
ABSTRACT
Pleurotus
pulmonarius crude aqueous (CA) extract
was previously reported to have therapeutic potential, thus its ability to
alleviate serum total cholesterol, vasodilation, and improve the aortic tissue
structure in hypercholesterolemia rat model was studied. Eight groups of
Wistar-Kyoto rats were involved including normal (G1), hypercholesterolemia
(G2), treatment (G3 to G5) and prevention (G6 to G8). Two doses of CA; 0.5 g/kg
body weight (BW), 2.0 g/kg BW, and simvastatin 10 mg/kg BW were orally fed to
the rats (G3 to G8). Treatment groups were induced with hypercholesterolemia
and later treated with CA/simvastatin, while prevention groups were given both
hypercholesterolemia-diet and CA/simvastatin simultaneously. The thoracic
aortic rings (TAR) were subjected to contractility study and histopathology
examination. In organ bath analysis, pre-contracted TAR of G1 with phenylephrine
(PE) achieved 60% vasodilation response towards acetylcholine (ACh) whereas TAR
of G2 unable to respond to ACh. G3 to G5 groups failed to dilate when induced
with ACh whereas G6 to G8 groups showed a slight improvement. The repeated
precontracted TAR of G1 and G2 significantly dilated with the presence of CA
and simvastatin. TAR of G1 achieved 100% vasodilation at 3.0 mg/mL CA and 2.4
mg/mL simvastatin. TAR of G2 achieved 73% vasodilation at 6.0 mg/mL CA whereas
76.8% dilation recorded for simvastatin at 4.8 mg/mL. Histopathological
examination found that CA was able to improve the structure of the aortic
cells. These observations suggest that CA helps to improve tissue condition and
vasodilation of the hypercholesterolemic aorta.
Keywords: Aortic ring; crude
aqueous; dilation; histopathology; hypercholesterolemia
ABSTRAK
Ekstrak
akueus mentah Pleurotus pulmonarius (CA) pernah dilaporkan sebelum ini
mempunyai potensi terapi, oleh itu keupayaannya untuk mengurangkan kolesterol
total serum, vasodilasi dan memperbaiki struktur tisu aorta pada model tikus
hiperkolesterolemia telah dikaji. Lapan kumpulan tikus Wistar-Kyoto yang
terlibat termasuklah normal (G1), hiperkolesterolemia (G2), rawatan (G3 hingga
G5) dan pencegahan (G6 hingga G8). Dua dos CA; 0.5 g/kg berat badan (BW), 2.0
g/kg BW dan simvastatin 10 mg/kg BW telah diberi makan secara oral kepada tikus
(G3 hingga G8). Kumpulan rawatan telah diaruh dengan hiperkolesterolemia dan
kemudian dirawat dengan CA/simvastatin, manakala kumpulan pencegahan telah
diberi diet-hiperkolesterolemia dan CA/simvastatin secara serentak. Cincin
aorta toraks (TAR) telah digunakan untuk kajian kontraktiliti dan pemeriksaan
histopatologi. Dalam analisis mandi organ, pra-kontrak TAR G1 dengan
fenilefrina
Kata kunci: Akueus mentah; cincin
aorta; dilat; hiperkolesterolemia; histopatologi
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*Corresponding author; email: hanizaaminudin@um.edu.my
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