Sains Malaysiana 51(1)(2022): 285-296

http://doi.org/10.17576/jsm-2022-5101-23

Oral Supplementation of β-Carotene Benefits the Hepatic Structure and Metabolism in Mice (Mus musculus) Exposed to A Chronic Ethanol Consumption

(Suplemen Oral β-Karotena Bermanfaat kepada Struktur Hepatik dan Metabolisme Tikus

(Mus musculus) Terdedah kepada Penggunaan Etanol Kronik)

CRISTIAN SANDOVAL^1,2*, BÉLGICA VÁSQUEZ³, ADRIANA VASCONCELLOS⁴, VANESSA SOUZA-MELLO⁵, KHOSROW ADELI⁶, CARLOS MANDARIM-DE-LACERDA⁵ & MARIANO DEL SOL⁷

¹Escuela de Tecnología Médica, Facultad de Salud, Universidad Santo Tomás, Osorno, Chile

²Departamento de Ciencias Preclínicas, Facultad de Medicina, Universidad de la Frontera, Temuco, Chile

³Facultad de Ciencias de La Salud, Universidad de Tarapacá, Arica, Chile

⁴Departamento de Ciencias Básicas, Facultad de Medicina, Universidad de La Frontera, Temuco, Chile

⁵Laboratorio de Morfometría, Metabolismo y Enfermedades Cardiovasculares, Centro Biomédico, Instituto de Biología, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brasil

⁶Molecular Medicine, Research Institute, The Hospital for Sick Children, University of Toronto, Toronto ON, Canada

⁷Centro de Excelencia en Estudios Morfológicos y Quirúrgicos (CEMyQ), Universidad de La Frontera, Temuco, Chile

Received: 18 March 2021/Accepted: 27 April 2021

ABSTRACT

Ethanol cannot be excreted and must be metabolized in the liver. Alcoholic liver disease is a blanket term for conditions related specifically to the liver and alcohol use. The study aimed to evaluate the metabolic, biochemical and histological effects of the oral supplementation with β-carotene on the liver of C57BL/6 mice exposed to ethanol consumption. Thirty male C57BL/6 mice (Mus musculus) were divided into six experimental groups: Control (C), Low-dose alcohol (LA), Moderate-dose alcohol (MA), β-carotene (B), Low-dose alcohol+β-carotene (LA+B) and Moderate-dose alcohol+β-carotene (MA+B) group. One-way ANOVA was used. The greatest intake of calories was noted in the LA (65.4 ± 12.5 kJ) and MA (68.6 ± 18.6 kJ) groups. The LA+B and MA+B groups shown an improvement in the HOMA-IR index (8.7 ± 2.4 and 6.7 ± 3.5, respectively), increased ADH levels (16.2 ± 1.6 and 18.9 ± 0.5 pmol/minmL^-1, respectively) and decreased insulin levels (14.0 ± 3.3 and 10.6 ± 5.7 µUmL^-1, respectively). It was also observed that oral supplementation with β-carotene improved the hepatic parenchyma in the LA+B group, showing normal-sized hepatocytes, whereas in the MA+B group it relieved the structural damage, revealing fewer lipid droplets than the MA group.

Keywords: Alcohol abuse; alcoholic liver disease; antioxidants; antioxidant effects

ABSTRAK

Etanol tidak boleh diekspresikan dan mesti dimetabolisme di dalam hati. Penyakit hati alkohol adalah istilah selimut untuk keadaan yang berkaitan secara khusus dengan penggunaan hati dan alkohol. Kajian ini bertujuan untuk menilai kesan metabolik, biokimia dan histologi suplemen dengan β-karotena pada hati tikus C57BL/6 yang terdedah kepada pengambilan etanol. Tiga puluh tikus C57BL/6 lelaki (Mus musculus) dibahagikan kepada enam kumpulan uji kaji: Kawalan (C), alkohol dos rendah (LA), alkohol dos sederhana (MA), β-karotena (B), alkohol dos rendah + kumpulan β-karotena (LA+B) dan alkohol dos sederhana+β-karotena (MA+B). ANOVA sehala telah digunakan. Pengambilan kalori paling banyak dicatat dalam kumpulan LA (65.4 ± 12.5 kJ) dan MA (68.6 ± 18.6 kJ). Kumpulan LA+B dan MA+B menunjukkan peningkatan dalam indeks HOMA-IR (8.7 ± 2.4 dan 6.7 ± 3.5), peningkatan kadar ADH (16.2 ± 1.6 dan 18.9 ± 0.5 pmol/minitmL^-1) dan penurunan tahap insulin (14.0 ± 3.3 dan 10.6 ± 5.7 µUmL^-1). Juga diperhatikan bahawa suplemen dengan β-karotena meningkatkan parenkima hepatik pada kumpulan LA+B, menunjukkan hepatosit berukuran normal, sedangkan dalam kumpulan MA+B ia melegakan kerosakan struktur, menunjukkan lebih sedikit titisan lipid daripada kumpulan MA.

Kata kunci: Antioksidan; kesan antioksidan; penderaan alkohol; penyakit hati alkohol

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*Corresponding author; email: cristian.sandoval@ufrontera.cl