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Sains Malaysiana 51(7)(2022):
http://doi.org/10.17576/jsm-2022-5107-14
Optimization
and Characterization of Fatty Acid Esters (FAES) Based Nanostructured Lipid
Carrier (NLC) by Box-Behnken Analysis
(Pengoptimuman dan Pencirian Ester Asid Lemak (FAES) Berasaskan Pembawa Lipid Berstruktur Nano (NLC) oleh Analisis Box-Behnken)
SARAH SHAZWANI S. & MISNI MISRAN*
Department of Chemistry, Faculty of Science, University
of Malaya, 50603
Kuala Lumpur, Federal Territory, Malaysia
Received: 29 August 2021/Accepted: 30 December 2021
An unfavorable rate of toxicity and hydrophobicity of active
substance in water has prompted the development of an improved active
ingredients delivery systems such as nanostructured lipid carriers (NLC). This
present study investigates varying components of NLCs compositions to achieve
an optimized and stable colloidal suspension of NLCs through Box-Behnken design
analysis for potential use as an active substance carrier system. The optimised
formulation is comprised of 2.9% stearic acid, 0.4% MCT, 0.3% IPM, 0.37% Tween
20, 0.23% Span 20 and 96% deionised water (DW). The mean particle size,
polydispersity index, and zeta potential of the optimized NLCs were 322±13.5 nm, 0.199±0.04, and -36±0.1 mV, respectively. Based
on the TEM micrograph, NLCs can be observed as having an elongated spherical
shape with a dense appearance.
Keywords: Box-Behnken design; isopropyl myristate; medium-chain triglyceride;
nanostructured lipid carrier; surfactant
Abstrak
Tahap ketoksikan dan sifat hidrofobik sesetengah bahan aktif yang kurang memberangsangkan telah mendorong kepada kajian berkenaan sistem penyampaian ubat yang lebih baik seperti pembawa lipid berstruktur nano (NLC). Justeru, penyelidikan ini mengkaji potensi NLC sebagai sistem pembawa ubat yang baik melalui penelitian kepelbagaian komponen komposisi NLC untuk mencapai suspensi koloid yang stabil dan optimum melalui analisis reka bentuk Box-Behnken. Formulasi yang dioptimumkan terdiri daripada 2.9% asid stearat, 0.4% MCT, 0.3% IPM,
0.37% Tween 20, 0.23% Span 20 dan 96% air deionisasi (DW). Saiz zarah min, indeks polidispersi dan potensi zeta NLC yang optimum masing-masing adalah 322±13.5 nm, 0.199±0.04 dan -36±0.1 mV. Berdasarkan mikrograf TEM, NLC dapat dilihat memiliki bentuk sfera memanjang dengan penampilan yang padat.
Kata kunci: Isopropil miristat; pembawa lipid berstruktur nano; reka bentuk Box-Behnken; surfaktan; trigliserida rantai sederhana (MCT)
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*Corresponding author; email: misni@um.edu.my
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