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Sains Malaysiana 51(7)(2022):
http://doi.org/10.17576/jsm-2022-5107-16
Combined Antioxidant Formulation of Ascorbic Acid
with Resveratrol Ameliorates Isoproterenol-Induced
Myocardial Infarction in Rats
(Gabungan Formulasi Antioksidan Asid Askorbik dengan Resveratrol yang Ditambah Baik dengan Isoproterenol-Penginfarkan Miokardium Teraruh dalam Tikus)
HAICHAO GAO1, HONG WANG1,*, TINGTING HAN2, XIANSHENG HUANG1,
SHUCHENG LI1 & XIBING WANG1
1Department of Cardiology, Affiliated Hospital of
Chengde Medical College, No 36, Nanyingzi Street 067000, Chengde, Hebei Province, China
2Department of Emergency, The Third Central Hospital
of Tianjin, 300170 Tianjin, China
Received: 31 July 2021/Accepted: 7 December 2021
ABSTRACT
The current investigation was
proposed to assess the effectiveness of the combination of resveratrol and
ascorbic acid against Isoproterenol (ISO)-induced myocardial infarction in
rats. The experimental model was divided
into six groups (n = 6 in each group). Group I: control, Group 2: isoproterenol
(ISO)-100 mg/kg b.wt, Group 3: ISO+Resveratrol (RES) (20 mg/kg b.wt) treated, Group 4: ISO+ Ascorbic
acid (AA) (80 mg/kg b.wt) treated, Group 5: ISO+RES
(20 mg/kg b.wt)+AA (80 mg/kg b.wt)
treated and Group 6 : RES (20 mg/kg b.wt)+ AA (80 mg/kg b.wt) alone
treated. The study showed an increase in lipid peroxides and cardiac markers in
the serum samples of experimental animals administered with ISO. Treatment with
RES and AA individually and a combinational formulation brought a significant
decrease in lipid peroxides and cardiac markers. They increased the level of
enzymatic antioxidants and non-enzymatic antioxidant levels. Histopathological
results showed the distortion of heart architecture among the experimental
groups administered with ISO and significant recovery when treated with RES and
AA individually and in their combination. The study presents the effective
combination of RES and AA in combating ISO-induced myocardial infarction and
protection against ROS-mediated oxidative stress.
Keywords: Ascorbic acid; isoproterenol; myocardial
infarction; resveratrol
ABSTRAK
Penyelidikan ini dicadangkan untuk menilai keberkesanan gabungan resveratrol dan asid askorbik terhadap penginfarkan miokardium yang disebabkan oleh Isoproterenol (ISO) pada tikus. Model uji kaji dibahagikan kepada enam kumpulan (n = 6 dalam setiap kumpulan). Kumpulan I: kawalan, Kumpulan 2: isoproterenol
(ISO)-100 mg/kg b.wt, Kumpulan 3: ISO+Resveratrol (RES) (20 mg/kg b.wt) terawat,
Kumpulan 4: ISO+ Askorbik asid (AA) (80 mg/kg b.wt) terawat,
Kumpulan 5: ISO+RES (20 mg/kg b.wt)+AA (80 mg/kg b.wt) terawat dan Kumpulan 6 : RES (20 mg/kg b. wt)+ AA (80
mg/kg b.wt) sahaja terawat. Kajian menunjukkan peningkatan dalam peroksida lipid dan penanda jantung dalam sampel serum haiwan uji kaji yang telah diberi ISO. Rawatan dengan
RES dan AA secara individu dan gabungan formulasi membawa penurunan yang ketara
dalam peroksida lipid dan penanda jantung. Ia meningkatkan tahap antioksidan berenzim dan tahap antioksidan bukan berenzim. Keputusan histopatologi mendedahkan herotan arkitektur jantung dalam kalangan kumpulan uji kaji yang diadministrasi dengan ISO dan
pemulihan yang ketara apabila dirawat dengan RES dan AA secara individu dan secara gabungan. Kajian itu membentangkan gabungan RES dan AA
yang berkesan dalam memerangi penginfarkan miokardium yang disebabkan oleh
ISO dan perlindungan terhadap tekanan oksidatif pengantaraan ROS.
Kata kunci: Asid askorbik;
isoproterenol; penginfarkan miokardium;
resveratrol
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*Corresponding author; email: cdwanghong666@sina.com
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