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Sains Malaysiana 51(9)(2022):
http://doi.org/10.17576/jsm-2022-5109-04
Variability of White Spot Syndrome Virus (WSSV)
Envelope Protein VP28 from Diseased Shrimp (Litopenaeus vannamei) in Indonesia
(KepelbagaianVirus Sindrom
Bintik Putih (WSSV) Protein Sampul VP28 daripada Udang (Litopenaeus vannamei) Berpenyakit di Indonesia)
A.C.M.A.R. TASAKKA1,*,
G. LATAMA1, A.A. HIDAYANI1, A. PARENRENGI2, A.
TENRIULO2, A.N.R. RELATAMI3, A.M. MOORE4 &
A. SHAHEEN5
1Department of Fisheries, Faculty of Marine Science and
Fisheries, Hasanuddin University, Makassar, South Sulawesi, Indonesia
2Research Center for Fishery, National Research and Innovation Agency, Indonesia
3Veterinary Medicine
Department, Faculty of Medicine, Hasanuddin University, Makassar,
South Sulawesi,
Indonesia
4Graduate School, Hasanuddin University, Makassar, South Sulawesi, Indonesia
5National
Institute of Oceanography, Karachi, Pakistan
Received: 13 December 2021/Accepted: 25 April 2022
Abstract
White Spot Disease (WSD) is a viral disease
affecting crustaceans. Caused by the White Spot Syndrome Virus (WSSV), this
disease has caused significant mortality in commercially cultivated marine
shrimp species with severe impacts on the shrimp farming industry and may be a
threat to wild shrimp stocks. Thorough studies on the molecular biology of this
pathogen are urgently needed to improve understanding of the virus at a
molecular level, including variation in key viral protein (VP) components of
the WSSV virion. This study aimed to isolate and characterize WSSV VP28 gene
encoding envelope proteins from Indonesian Pacific white shrimp (Litopenaeus vannamei)
isolates. Infected juvenile shrimp were collected from Pangkep, Barru, and Pinrang Districts in South Sulawesi, Indonesia. Genomic DNA was isolated from infected
shrimp muscle tissue using a DTAB-CTAB (dodecyle trimethyl ammonium bromide-hexadecyl trimethyl ammonium
bromide) DNA extraction procedure. The WSSV VP28 DNA
sequences from Pangkep, Barru,
and Pinrang isolates were 640-680 bp in length.
Homology of Pangkep isolates with isolates from Barru and Pinrang was 97-99%.
BLAST-N (Basic Local Alignment Search Tool-Nucleotide) analysis showed isolates
from all three sites clustered with WSSV VP28 accessions from China, Indonesia,
Japan, South Carolina and Vietnam. These results increase the geographic spread
and host taxon coverage of WSSV VP28 sequence data for Indonesia.
Keywords: Litopenaeus vannamei; South Sulawesi; VP28;
viral protein variability; WSSV
Abstrak
Penyakit Bintik
Putih (WSD) adalah penyakit virus yang menjejaskan krustasea. Disebabkan oleh
Virus Sindrom Bintik Putih (WSSV), penyakit ini telah menyebabkan kematian yang
ketara dalam spesies udang marin yang diusahakan secara komersial dengan kesan
yang teruk terhadap industri penternakan udang dan mungkin menjadi ancaman
kepada stok udang liar. Kajian menyeluruh tentang biologi molekul patogen ini
amat diperlukan untuk meningkatkan pemahaman virus pada tahap molekul, termasuk
variasi dalam komponen protein virus (VP) utama virion WSSV. Kajian ini
bertujuan untuk mengasingkan dan mencirikan gen WSSV VP28 pengekodan protein
sampul surat daripada pencilan udang putih Pasifik Indonesia (Litopenaeus vannamei). Udang juvana yang
dijangkiti dikumpul dari Daerah Pangkep, Barru dan Pinrang di Sulawesi Selatan,
Indonesia. DNA genom telah diasingkan daripada tisu otot udang yang dijangkiti
menggunakan prosedur pengekstrakan DNA DTAB-CTAB (dodecyle trimethyl ammonium bromide-hexadecyl trimethyl ammonium
bromide). Urutan DNA WSSV VP28 daripada pencilan Pangkep, Barru dan Pinrang
adalah 640-680 bp panjang. Homologi pencilan Pangkep dengan pencilan Barru dan
Pinrang adalah 97-99%. Analisis BLAST-N (Alat Carian Penjajaran Tempatan
Asas-Nukleotida) menunjukkan pencilan daripada ketiga-tiga tapak berkelompok
dengan aksesi WSSV VP28 dari China, Indonesia, Jepun, Carolina Selatan dan
Vietnam. Hasil ini meningkatkan sebaran geografi dan liputan takson hos bagi
data jujukan WSSV VP28 untuk Indonesia.
Kata kunci:
Kebolehubahan protein virus; Litopenaeus vannamei; Sulawesi Selatan;
VP28; WSSV
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*Corresponding author;
email: citra@unhas.ac.id
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